Korean Journal of Head & Neck Oncology (대한두경부종양학회지)

The Korean Society for Head and Neck Oncology (대한두경부종양학회)
권호 표지

The Effect of Cancer Associated Fibroblasts(CAFs) and CD44 of CAFs on the Motility of Cancer Cells in Head and Neck Squamous Cell Carcinoma

두경부편평세포암에서 암-연관 섬유아세포가 암세포의 운동성에 미치는 영향과 암-연관 섬유아세포에서 과발현되는 CD44의 역할

  • Shim, Seon-Hui (Department of Molecular Tumor Biology, Seoul National University College of Medicine) ;
  • Hah, J. Hun (Cancer Research Institute, Seoul National University College of Medicine) ;
  • Cho, Soo Youn (Department of Pathology, Korea Cancer Center Hospital) ;
  • Kim, Tae-Min (Department of Internal Medicine, Seoul National University Hospital) ;
  • Koh, Young-Il (Department of Internal Medicine, Seoul National University Hospital) ;
  • Kim, Dong-Wan (Cancer Research Institute, Seoul National University College of Medicine) ;
  • Lee, Choon-Taek (Department of Medicine and Respiratory Center, Seoul National University Bundang Hospital) ;
  • Heo, Dae Seog (Department of Molecular Tumor Biology, Seoul National University College of Medicine) ;
  • Sung, Myung-Whun (Department of Molecular Tumor Biology, Seoul National University College of Medicine)
  • 심선희 (서울대학교 의과대학 분자종양생물학교실) ;
  • 하정훈 (서울대학교 의과대학 암연구소) ;
  • 조수연 (서울대학교 의과대학 원자력병원 병리과) ;
  • 김태민 (서울대학교병원 내과) ;
  • 고영일 (서울대학교병원 내과) ;
  • 김동완 (서울대학교 의과대학 암연구소) ;
  • 이춘택 (서울대학교분당병원 내과 및 폐센터) ;
  • 허대석 (서울대학교 의과대학 분자종양생물학교실) ;
  • 성명훈 (서울대학교 의과대학 분자종양생물학교실)
  • Received : 2014.09.29
  • Accepted : 2014.10.02
  • Published : 2014.10.31
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Abstract

배경 및 목적 암-연관 섬유아세포(Cancer-associated fibroblasts, CAF)는 종양미세환경의 가장 중요한 요소의 하나다. 그래서 두경부편평세포암에 대해 CAF가 암세포의 운동성에 미치는 영향을 평가하고, CAF에 과발현되는 CD44의 역할에 대해 평가하고자 하였다. 재료 및 방법 두경부암환자의 종양조직에서 CAF를 분리하고, 비종양성 조직으로부터 정상 섬유아세포(NHF)를 분리하였다. 창상치유분석과 상하 챔버를 이용한 3차원 세포 이동 분석을 이용하여, CAF가 암세포의 이동에 미치는 영향을 분석하고, CAF에서 과발현되는 CD44를 중화항체로 CAF를 차단했을 때 암세포 이동의 변화를 관찰하였다. 결과 NHF에 비해 CAF에서 CD44가 과발현되는 것을 관찰하였다. 창상치유분석에서 CAF와 같이 배양된 암세포는 NHF와 같이 배양된 암세포에 비해 더 빠른 이동을 보였다. CD44 중화 항체를 처리했을 때는 암세포의 이동성이 저해되었다. 결론 CAF는 종양미세환경에서 암세포의 운동성을 조절하는 중요한 인자의 하나일 것으로 사료된다. CD44는 CAF의 기능을 매개하는 중요한 표지자 중 하나로 생각된다.

Keywords

References

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