Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Effect of Mixture of Lactobacillus plantarum CECT 7527, 7528, and 7529 on Obesity and Lipid Metabolism in Rats Fed a High-fat Diet

Lactobacillus plantarum CECT 7527, 7528, 7529 혼합물이 고지방식이를 급여한 흰쥐의 비만 및 지질 대사에 미치는 영향

  • Kim, Dae Hwan (Center for Efficacy Assessment and Development of Functional Foods and Drugs) ;
  • Choi, Mi-Ran (Center for Efficacy Assessment and Development of Functional Foods and Drugs) ;
  • Hong, Ji Eun (Center for Efficacy Assessment and Development of Functional Foods and Drugs) ;
  • Lee, Jae-Yong (Center for Efficacy Assessment and Development of Functional Foods and Drugs) ;
  • Lee, Soon Im (Business Development Department, HanAll BioPharma Co. Ltd.) ;
  • Jung, Su Hwan (Business Development Department, HanAll BioPharma Co. Ltd.) ;
  • Kim, Eun Ji (Center for Efficacy Assessment and Development of Functional Foods and Drugs)
  • 김대환 (한림대학교 식의약품의 효능평가 및 기능성소재개발센터) ;
  • 최미란 (한림대학교 식의약품의 효능평가 및 기능성소재개발센터) ;
  • 홍지은 (한림대학교 식의약품의 효능평가 및 기능성소재개발센터) ;
  • 이재용 (한림대학교 식의약품의 효능평가 및 기능성소재개발센터) ;
  • 이순임 (한올바이오파마 주식회사 개발팀) ;
  • 정수환 (한올바이오파마 주식회사 개발팀) ;
  • 김은지 (한림대학교 식의약품의 효능평가 및 기능성소재개발센터)
  • Received : 2014.05.22
  • Accepted : 2014.07.16
  • Published : 2014.10.31
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Abstract

In the present study, we examined the effects of a mixture of three strains, Lactobacillus plantarum CECT 7527, 7528, and 7529 (L. plantarum mixture), on body weight and lipid metabolism in Sprague-Dawley rats fed a high-fat diet. Rats were fed a high-fat diet and subjected to oral gavage with vehicle or the L. plantarum mixture ($0.6{\times}10^9$, $1.2{\times}10^9$, $2.4{\times}10^9$ colony-forming units (CFU)/day/rat, respectively) for 8 weeks. In rats fed a high-fat diet, oral administration of $2.4{\times}10^9CFU/day$ of the L. plantarum mixture significantly reduced body weight gain as well as weights of liver and epididymal fat. Leptin levels in sera were significantly reduced by oral administration of $2.4{\times}10^9CFU/day$ of the L. plantarum mixture. The L. plantarum mixture ($1.2{\times}10^9$ or $2.4{\times}10^9CFU/day$) also reduced the concentrations of total cholesterol and LDL-cholesterol in sera when it administered orally. Further, the L. plantarum mixture significantly reduced the atherogenic index and cardiac risk factor. In addition, oral administration of $2.4{\times}10^9CFU/day$ of the L. plantarum mixture markedly reduced levels of total lipids, triglycerides, and total cholesterol in the liver. The results of this study indicate that the L. plantarum mixture may exhibit anti-obesity and cholesterol-lowering effects, which suggest that the L. plantarum mixture has the potential to be a probiotic in the management of obesity and hypercholesterolemia.

본 연구에서는 고지방식이 투여로 비만 및 고지혈증을 유도한 동물에서 L. plantarum CECT 7527, 7528과 7529 혼합물이 비만 및 고지혈증에 미치는 영향을 조사하고자 하였다. 흰쥐에 8주 동안 고지방식이를 급여하면서 L. plantarum 혼합물을 $0.6{\sim}2.4{\times}10^9CFU/day$를 경구 투여하였고 동물을 희생하여 혈청 및 간조직 내의 지질 함량을 조사하였다. L. plantarum 혼합물 투여에 의해 체중증가량, 간과 부고환 지방 무게가 현저히 감소하였다. 또한 비만과 관련 있는 호르몬인 혈청 렙틴의 함량이 L. plantarum 혼합물 투여에 의해 감소하였다. L. plantarum 혼합물 투여가 혈청 중성지방과 HDL-콜레스테롤 함량 및 혈당에는 영향을 미치지 않았으나 혈청 총콜레스테롤 함량 및 LDL-콜레스테롤 함량을 현저히 감소하였다. 이와 더불어 L. plantarum 혼합물 투여가 동맥경화지수와 심혈관위험지수를 유의적으로 감소하였다. 간조직 내의 총 지질 함량, 중성지방 함량 및 총콜레스테롤 함량은 L. plantarum 혼합물 투여에 의해 유의적으로 감소하였다. 이상의 결과는 L. plantarum CECT 7527, 7528과 7529가 혼합된 L. plantarum 혼합물이 체지방 감소 및 고지혈증 개선 효과를 나타냄을 보여준다. 향후 더 많은 연구가 필요하나 본 연구는 L. plantarum 혼합물을 비만 및 고지혈증 개선제로 개발할 수 있는 가능성을 제시한다.

Keywords

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