Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Analysis of Pine Nut Oil Composition and Its Effects on Obesity

잣기름 성분분석 및 비만 예방효과 연구

  • Kim, Kyoung Kon (Department of Bio-Health Technology, Kangwon National University) ;
  • Kang, Yun Hwan (Well-being Bioproducts RIC, Kangwon National University) ;
  • Kim, Dae Jung (Well-being Bioproducts RIC, Kangwon National University) ;
  • Kim, Tae Woo (Well-being Bioproducts RIC, Kangwon National University) ;
  • Choe, Myeon (Department of Bio-Health Technology, Kangwon National University)
  • 김경곤 (강원대학교 생명건강공학과) ;
  • 강윤환 (강원대학교 강원웰빙특산물산업화지역혁신센터) ;
  • 김대중 (강원대학교 강원웰빙특산물산업화지역혁신센터) ;
  • 김태우 (강원대학교 강원웰빙특산물산업화지역혁신센터) ;
  • 최면 (강원대학교 생명건강공학과)
  • Received : 2014.05.14
  • Accepted : 2014.07.01
  • Published : 2014.10.31
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Abstract

Pine nut oil (PNO) is well known to impart beneficial effects in overweight individuals, but the mechanisms underlying PNO-mediated weight loss remain unclear. To investigate how PNO promotes weight loss, its composition was determined by gas chromatography coupled with mass spectrometry (GC-MS). In addition, the effects of PNO on cytotoxicity, lipid accumulation, expression of lipid metabolism-related biomarkers, and leptin secretion were assessed in 3T3-L1 cells. GC-MS analyses revealed that PNO contains several components, including linoleic acid, oleic acid, palmitic acid, and stearic acid. Moreover, PNO did not have a cytotoxic effect on 3T3-L1 cells. However, it inhibited the expression of peroxisome proliferator-activated receptor (PPAR) and adipocyte protein 2 (aP2). Finally, PNO significantly increased leptin secretion in a dose-dependent manner. Taken together, these results support the notion that PNO is useful for weight management in overweight individuals.

본 연구에서는 성분분석을 통해 품질이 확인된 PNO의 항비만효과를 세포수준에서 확인하고 기능성소재로서의 연구 가능성을 제시하고자 수행하였다. 그 결과 PNO는 기존에 알려진 바와 같이 linoleic acid, oleic acid, pinolenic acid, 및 palmitic acid가 분석되었으며 정량을 위한 표준물질인 undecanoic acid의 함량을 기준으로 확인한 결과 linoleic acid 43.4%, oleic acid 25.7%, pinolenic acid로 예측되는 11번 unknown peak 12.8%, palmitic acid 5.2% 및 다수의 미량 성분을 함유하고 있었다. 세포독성 실험을 통해 0.2 mg/mL 농도 까지 독성이 관찰되지 않음을 확인하였으며 그 이상의 농도에서도 PNO보다는 용매 DMSO에 의한 독성이 관찰되었다. PNO를 이용하여 항비만 효과에 대한 잠재적 기능 및 기전을 연구하기 위해 Oil Red O staining, $PPAR{\gamma}$ 및 aP2 mRNA 발현 확인 그리고 leptin의 분비량 측정을 진행하였다. 그 결과 PNO는 MDI solution 처리를 통해 분화가 유도된 mature adipocyte에서 지방적(lipid droplet) 형성을 유의하게 억제하는 것을 확인할 수 있었으며 이는 PNO가 항비만 활성에 잠재적으로 기능을 할 수 있음을 의미한다. 또한 $PPAR{\gamma}$와 aP2 mRNA 발현을 확인한 결과, 두 유전자가 유의한 수준으로 MDI control 대비 감소하는 반면 SREBP-1c, $C/EBP{\alpha}$, glucokinase, phosphoenolpyruvate carboxykinase 및 perilipin에는 영향이 없는 것을 확인할 수 있었다(data not shown). RT-PCR 결과는 PNO의 처리가 지방대사 및 축적을 단백질 및 유전자 발현조절 기전에 의해 억제할 수 있음을 제시하여 주며, 추가적인 연구가 필요함을 시사한다. 마지막으로 확인한 PNO의 leptin 방출유도 실험을 통해 leptin이 PNO의 처리에 의해 농도 의존적으로 방출량이 증가됨을 확인할 수 있었다. Leptin은 식욕억제 효과가 있는 호르몬으로 잘 알려져 있다. 이는 PNO가 기존에 알려진 cholecystokinin과 glucagon like peptide-1 (GLP-1)의 분비증가를 통해 식욕을 억제할 뿐만 아니라 leptin의 방출을 유도하여 추가적인 식욕억제작용을 유도할 수 있음을 의미한다. 위의 연구결과를 종합하면 PNO는 불포화지방산이 다량 함유된 우수한 식물성유지이며 기능적으로 항비만 효능을 잠재적으로 가지는 우수한 소재임을 확인할 수 있었다. 따라서 본 연구결과를 기초자료로 하여 효과적인 기능성 식품 및 소재의 개발을 위해 체계적이고 과학적인 연구가 필요할 것으로 판단된다.

Keywords

References

  1. Kim J, Im S, Choi SH, Lee JS, Roh MS, Lim YP. Construction of a full-length cDNA library from Pinus koraiensis and analysis of EST dataset. CNU J. Agr. Sci. 38: 11-16 (2011)
  2. Yoon TH. Fatty acid composition of total lipids from seeds of Pinus koraiensis. J. Korean Soc. Food Nutr. 16: 93-97 (1987)
  3. Park S, Lim Y, Shin S, Han SN. Impact of Korean pine nut oil on weight gain and immune responses in high-fat diet-induced obese mice. Nutr. Res. Pract. 7: 352-358 (2013) https://doi.org/10.4162/nrp.2013.7.5.352
  4. Sales-Campos H, Souza PR, Peghini BC, da Silva JS, Cardoso CR. An overview of the modulatory effects of oleic acid in health and disease. Mini Rev. Med. Chem. 13: 201-210 (2013)
  5. Zhao G, Etherton TD, Martin KR, Vanden Heuvel JP, Gillies PJ, West SG, Kris-Etherton PM. Anti-inflammatory effects of polyunsaturated fatty acids in THP-1 cells. Biochem. Biophys. Res. Commun. 336: 909-917 (2005) https://doi.org/10.1016/j.bbrc.2005.08.204
  6. Lee JW, Lee KW, Lee SW, Kim IH, Rhee C. Selective increase in pinolenic acid (all-cis-5,9,12-18:3) in Korean pine nut oil by crystallization and its effect on LDL-receptor activity. Lipids 39: 383-387 (2004) https://doi.org/10.1007/s11745-004-1242-2
  7. Chen SJ, Hsu CP, Li CW, Lu JH, Chuang LT. Pinolenic acid inhibits human breast cancer MDA-MB-231 cell metastasis in vitro. Food Chem. 126: 1708-1715 (2011) https://doi.org/10.1016/j.foodchem.2010.12.064
  8. Hughes GM, Boyland EJ, Williams NJ, Mennen L, Scott C, Kirkham TC, Harrold JA, Keizer HG, Halford JC. The effect of Korean pine nut oil (PinnoThinTM) on food intake, feeding behaviour and appetite: a double-blind placebo-controlled trial. Lipids Health Dis. 7: 6 (2008) https://doi.org/10.1186/1476-511X-7-6
  9. Le NH, Shin S, Tu TH, Kim CS, Kang JH, Tsuyoshi G, Teruo K, Han SN, Yu R. Diet enriched with korean pine nut oil improves mitochondria oxidative metabolism in skeletal muscle and brown adipose tissue in diet-induced obesity. J. Agr. Food Chem. 60: 11935-11941 (2012) https://doi.org/10.1021/jf303548k
  10. Pasman WJ, Heimerikx J, Rubingh CM, van den Berg R, O'Shea M, Gambelli L, Hendriks HF, Einerhand AW, Scott C, Keizer HG, Mennen LI. The effect of Korean pine nut oil on in vitro CCK release, on appetite sensations and on gut hormones in post-menopausal overweight women. Lipids Health Dis. 7: 10 (2008) https://doi.org/10.1186/1476-511X-7-10
  11. Yu JH, Kim MS. Molecular mechanisms of appetite regulation. Diabetes Metab. J. 36: 391-398 (2012) https://doi.org/10.4093/dmj.2012.36.6.391
  12. Fisher RM, Eriksson P, Hoffstedt J, Hotamisligil GS, Thrne A, Rydn M, Hamsten A, Arner P. Fatty acid binding protein expression in different adipose tissue depots from lean and obese individuals. Diabetologia. 44: 1268-1273 (2001) https://doi.org/10.1007/s001250100643
  13. Furuhashi M, Tuncman G, Grgn CZ, Makowski L, Atsumi G, Vaillancourt E, Kono K, Babaev VR, Fazio S, Linton MF, Sulsky R, Robl JA, Parker RA, Hotamisligil GS. Treatment of diabetes and atherosclerosis by inhibiting fatty-acid-binding protein aP2. Nature 447: 959-965 (2007) https://doi.org/10.1038/nature05844
  14. Nugara RN, Inafuku M, Iwasaki H, Oku H. Partially purified Peucedanum japonicum Thunb extracts exert anti-obesity effects in vitro. Nutrition 30: 575-583 (2014) https://doi.org/10.1016/j.nut.2013.09.017
  15. Watkins BA, Shen CL, McMurtry JP, Xu H, Bain SD, Allen KG, Seifert MF. Dietary lipids modulate bone prostaglandin E2 production, insulin-like growth factor-I concentration and formation rate in chicks. J. Nutr. 127: 1084-1091 (1997)
  16. Ministry of Food and Drug Safety. Food Code. Available from: http://fse.foodnara.go.kr/residue/RS/jsp/menu_02_01_01.jsp. Accessed April. 17, 2014.
  17. Magun R, Gagnon A, Yaraghi Z, Sorisky A. Expression and regulation of neuronal apoptosis inhibitory protein during adipocyte differentiation. Diabetes 47: 1948-1952 (1998) https://doi.org/10.2337/diabetes.47.12.1948
  18. Yeh WC, Bierer BE, McKnight SL. Rapamycin inhibits clonal expansion and adipogenic differentiation of 3T3-L1 cells. Proc. Natl. Acad. Sci. U.S.A. 92: 11086-11090 (1995) https://doi.org/10.1073/pnas.92.24.11086
  19. Kang ES, Ham SA, Hwang JS, Lee CK, Seo HG. Effects of Garcinia cambogia extract on the adipogenic differentiation and lipotoxicity. Korean J. Food Sci. An. 33: 411-416 (2013) https://doi.org/10.5851/kosfa.2013.33.3.411
  20. Lee SM, Kang YH, Kim DJ, Kim KK, Lim JG, Kim TW, Choe M. Comparison of antioxidant and glucosidase inhibition activities among water extracts and sugar immersion extracts of green pepper, purslane and shiitake. J. East Asian Soc. Dietary Life. 24: 101-108 (2014)
  21. Baek J, Lee J, Kim K, Kim T, Kim D, Kim C, Tsutomu K, Ochir S, Lee K, Park CH, Lee YJ, Choe M. Inhibitory effects of Capsicum annuum L. water extracts on lipoprotein lipase activity in 3T3-L1 cells. Nutr. Res. Pract. 7: 96-102 (2013) https://doi.org/10.4162/nrp.2013.7.2.96
  22. Feng Z, Hai-ning Y, Xiao-man C, Zun-chen W, Sheng-rong S, Das UN. Effect of yellow capsicum extract on proliferation and differentiation of 3T3-L1 preadipocytes. Nutrition. 30: 319-325 (2014) https://doi.org/10.1016/j.nut.2013.08.003
  23. Acheampong A, Leveque N, Tchapla A, Heron S. Simple complementary liquid chromatography and mass spectrometry approaches for the characterization of triacylglycerols in Pinus koraiensis seed oil. J. Chromatogr. A. 1218: 5087-5100 (2011) https://doi.org/10.1016/j.chroma.2011.05.064
  24. Wolff RL, Bayard CC. Fatty acid composition of some pine seed oils. J. Am. Oil. Chem. Soc. 72: 1043-1046 (1995) https://doi.org/10.1007/BF02660719
  25. White UA, Stephens JM. Transcriptional factors that promote formation of white adipose tissue. Mol. Cell Endocrinol. 318: 10-14 (2010) https://doi.org/10.1016/j.mce.2009.08.023
  26. Ahmadian M, Suh JM, Hah N, Liddle C, Atkins AR, Downes M, Evans RM. PPAR$\gamma$ signaling and metabolism: the good, the bad and the future. Nat. Med. 19: 557-566 (2013)
  27. Nakamura MT, Yudell BE, Loor JJ. Regulation of energy metabolism by long-chain fatty acids. Prog. Lipid Res. 53: 124-144 (2014) https://doi.org/10.1016/j.plipres.2013.12.001