Journal of Mushroom (한국버섯학회지)

The Korean Society of Mushroom Science (한국버섯학회)
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Cultural characteristics and phylogenetic relationships based on RFLP analysis in Pleurotus species

  • Choi, Sun-Gyu (Mushroom Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Jang, Kab-Yeul (Mushroom Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Kim, Gyu-Hyun (Dep. of Biotechnology, Cheonan Yonam College) ;
  • Kong, Won-Sik (Mushroom Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Jo, Jae-Sun (Dep. of Food Science and Biotechnology, Kyung Hee University) ;
  • Kim, Hae-Yeong (Dep. of Food Science and Biotechnology, Kyung Hee University) ;
  • Yoo, Young-Bok (Mushroom Research Division, National Institute of Horticultural & Herbal Science, RDA)
  • Received : 2014.07.28
  • Accepted : 2014.08.27
  • Published : 2014.09.30
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Abstract

Pleurotus has increased rapidly production and consumption because of highly nutritional value, natural healthy food and so on. The basic studies for Pleurotus need for development of mushroom industry. This study was to investigate the cultural characteristics among 15 strains of 6 species and to analyze their phylogenetic relationships. The cultural characteristics were investigated by mycelial growth activity at different media, temperature and pH. The optimum media for mycelial growth were YM and MCM in most species. The optimum temperature for mycelial growth were $25^{\circ}C$ and $30^{\circ}C$. The optimum pH for mycelial growth were widly range from pH 5.1 to 7.4. Through the RFLP (restriction fragment length polymorphism) of IGS (intergenic spacer) I region in ribosomal DNA, it was analyzed phylogeny of interspecies and intraspecies. Each species was discriminated well as isolates within each species formed clade to be distinguished other species. P. florida was highly similar to P. floridanus, and P. flabellatus was P. cornucopiae. P. fuscus var. ferulae was highly similar to P. eryngii but discriminated different species in analysis of RFLP of IGS I region and showed different characteristics in mycelial culture. RFLP of IGS I region was useful of studying phylogenetic relationships of species and population.

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References

  1. Anderson JB, Stasovski E. 1992. Molecular physiology of northern hemisphere species of Armillaria. Mycologia 84: 505-516 https://doi.org/10.2307/3760315
  2. Appel DJ, Gordon TR. 1995. Intraspecific Variation within Populations of Fusarium oxysporum Based on RFLP Analysis of the Intergenic Spacer Region of rDNA. Exper. Mycol. 19, 120-128 https://doi.org/10.1006/emyc.1995.1014
  3. Bezalel L, Hadar Y, Cerniglia CE. 1997. Enzymatic mechanisms involved in phenanthrene degradation by the white rot fungus Pleurotus ostreatus. Appl. Environ. Microbiol. 63: 2495-2501
  4. Bobek P, Ozdin L. 1994. The mushroom Pleurotus ostreatus accelerates plasma very low-density lipoprotein clearance in hypercholesterolemic rat. Physiol. Res., 43:205-206
  5. Booth T. 1971. Chapter II. fungal culture media in Methods. Pp.49-94. In: Microbiology vol 4. Academic Press. London and New York
  6. Bruns TD, Fofel R, Talylor JW. 1990. Amplification and sequencing of DNA from fungi herbarium speciemens. Mycologia 82: 175-184 https://doi.org/10.2307/3759846
  7. Duchesne LC, Anderson JB. 1990. Location and direction of transcription of the 5S rRNA gene in Armillaria. Mycol. Res. 94: 266-269 https://doi.org/10.1016/S0953-7562(09)80626-6
  8. Forster H, Coffey MD, Elwood H, Sogin ML. 1990. Sequence analysis of the small subunit ribosomal RNAs of three zoosporic fungi and implications for fungal evolution. Mycologia 82: 306-312 https://doi.org/10.2307/3759901
  9. Giardina P, Cannio R, Martirani L, Marzullo L., Palmieri G, Sannia G. 1995. Cloning and sequencing of a laccase gene from the lignin-degrading basidiomycete Pleurotus ostreatus. Appl. Envir. Microbiol, 61: 2408-2413
  10. Graham GC. 1994. A simplified method for the preparation of fungal genomic DNA for PCR and RAPD analysis. Biotech. 16(1): 49-50
  11. Hong SB, Jee HJ, Lee SI, Go SJ, Rye JC, Kim IS. 1998. Three intraspecific groups in Korean isolates of Phytophthora drechsleri based on PCR-RFLP of ribosomal DNA. Korean J. Plant Pathol. 14(5): 519-525
  12. Illingworth CA, Andrews JH, Bibeau C and Sogin ML. 1991. Phylogenetic placement of Athelia bomvacian, Aureobasidium pullulans inferred from sequence comparisons of small subunit RNAs. Exper. Mycol. 15: 65-75 https://doi.org/10.1016/0147-5975(91)90008-2
  13. Johnson LF, Carl EA. 1972. Chapter 16 Culture media. Pp.187-208. In: Methods for research on the ecology of soil-borne plant pathogens.
  14. Kurashige S, Akuzawa Y, Endo F. 1997. Effects of Lentinus edodes, Glifola frondosa, and Pleurotus ostreatus admistration on cancer outbreak, and activities of macrophages and lymphocytes in mice treated with a carcinogen, n-butyl-n-butanolnitrosamine, Immunotoxicol. 192: 175-183
  15. Magee BB, D'souza TM, Magee PT. 1987. Strain and Species Identification by Restriction Fragment Length Polymorphisms in the Ribosomal DNA Repeat of Candida Species. J. Bacteriology. 169(4): p1639-1643 https://doi.org/10.1128/jb.169.4.1639-1643.1987
  16. Marzullo L, Cannio R, Giardina P, Santini MT, Sannia G. 1995. Veratryl alcohol oxidase from Pleurotus ostreatus participates in lignin biodegradation and prevents polymerization of laccase-oxidized substrates. J. Biol. Chem. 270: 3823-3827 https://doi.org/10.1074/jbc.270.8.3823
  17. Nei M, Li WH. 1979. Mathmatical model for studying genetic variation in terms of restriction endonucleases. Proc. Nat. Acad. Sci. 76: 5269-5273 https://doi.org/10.1073/pnas.76.10.5269
  18. Peberdy JF, Hanifah AH, Jia JH. 1993. New perspectives on the genetics of Pleurotus. Pp.55-62. In: Mushroom biology and mushroom products. Eds Chang, S. T., Buswell, J. A., and Chiu, S. W. The Chinese University Press. Hong Kong. China
  19. Raper CA, Raper JR, Miller RE. 1972. Genetic analysis of the life cycle of A. bisporus. Mycologia 64: 1088-1117. https://doi.org/10.2307/3758075
  20. Sambrook J, Russel DW. 2001. Molecular cloning: a laboratory manual. 3th ed. Cold Spring Harbor Laboratory. Cold Spring Harbor. New York. USA
  21. Shin KS, Oh IK, Kim CJ. 1997. Production and purification of Remazol-Brilliant-Blue-R decolorizing peroxidase from the culture filtrate of Pleurotus ostreatus. Appl. Environ. Microbiol., 63: 1744-1748
  22. Sneath PHA, Sokal RR. 1973. Numerical taxonomy. In: The principles and practices of numerical classification. W. H. Freeman and Company. San freancisco. USA