한국식품영양학회지 (The Korean Journal of Food And Nutrition)

  • 제27권5호
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  • Pages.845-850
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  • 2014
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  • 1225-4339(pISSN)
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  • 2287-4992(eISSN)
한국식품영양학회 (The Korean Society of Food and Nutrition)
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HaCaT 세포에서 자외선 B에 의해 유도되는 DNA 상해에 대한 국내 수종 자생 식물 에탄올 추출물의 저해효과

Inhibitory Effects of Ethanol Extracts of Some Korean Plants on Ultraviolet B-Induced DNA Damage

  • 이석희 (원광대학교 생명과학부 기초자연과학연구소) ;
  • 하세은 (원광대학교 생명과학부 기초자연과학연구소) ;
  • 조형권 (한풍제약) ;
  • 박종군 (원광대학교 생명과학부 기초자연과학연구소)
  • Lee, Seok Hee (Research Institute for Basic Science and Division of Biological Science, Wonkwang University) ;
  • Ha, Se Eun (Research Institute for Basic Science and Division of Biological Science, Wonkwang University) ;
  • Cho, Hyoung Kwon (Hanpoong Pharm & Foods Co., Ltd.) ;
  • Park, Jong Kun (Research Institute for Basic Science and Division of Biological Science, Wonkwang University)
  • 투고 : 2014.07.30
  • 심사 : 2014.09.30
  • 발행 : 2014.10.31
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초록

In the present study, the effects of extracts from Korean plants on the DNA damage response in HaCaT cells exposed to ultraviolet B (UVB) were investigated. The activity of cells treated for 24 hr with ethanol extracts from Vaccinium spp. (VS), and Vitis vinifera L (VV) alone was similar to that of the non-treated control, but gradually decreased at concentrations above $200{\mu}g/mL$. However, when post-incubation of UVB-exposed cells was carried out for 24 hr in medium containing VS or VV extracts, the cell activity increased in a concentration-dependent manner compared with that in the normal growth medium. The cell viability of UVB-exposed cells also increased when post-incubated in medium containing VS or VV extracts, in a concentration-dependent manner. Nuclear fragmentation analysis showed that post-incubation with VS or VV extracts decreased the UVB-induced apoptosis by about 10 and 13%, respectively, of that in cells post-incubated in growth medium. After 24 hr of post-incubation in medium containing VS or VV extracts, the level of CPD and 8-OHdG decreased in time- and concentration-dependent manners. Overall these results suggest that VS and VV extracts assist the survival of UVB-exposed cells, in accordance with the respective decrease in the levels of UVB-induced DNA damage.

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