Tuberculosis and Respiratory Diseases

The Korean Academy of Tuberculosis and Respiratory Diseases (대한결핵및호흡기학회)
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Effects of Morus alba L. and Natural Products Including Morusin on In Vivo Secretion and In Vitro Production of Airway MUC5AC Mucin

  • Lee, Hyun Jae (Department of Pharmacology, Chungnam National University School of Medicine) ;
  • Ryu, Jiho (Department of Pharmacology, Chungnam National University School of Medicine) ;
  • Park, Su Hyun (Department of Pharmacology, Chungnam National University School of Medicine) ;
  • Woo, Eun-Rhan (Department of Pharmacy, College of Pharmacy, Chosun University) ;
  • Kim, A Ryun (Department of Pharmacy, College of Pharmacy, Chosun University) ;
  • Lee, Sang Kook (Department of Pharmacy, College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University) ;
  • Kim, Yeong Shik (Department of Pharmacy, College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University) ;
  • Kim, Ju-Ock (Pulmonology Section, Department of Internal Medicine, Chungnam National University Hospital, Chungnam National University School of Medicine) ;
  • Hong, Jang-Hee (Department of Pharmacology, Chungnam National University School of Medicine) ;
  • Lee, Choong Jae (Department of Pharmacology, Chungnam National University School of Medicine)
  • Received : 2014.02.12
  • Accepted : 2014.05.21
  • Published : 2014.08.29
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Abstract

Background: It is valuable to find the potential activity of regulating the excessive mucin secretion by the compounds derived from various medicinal plants. We investigated whether aqueous extract of the root bark of Morus alba L. (AMA), kuwanon E, kuwanon G, mulberrofuran G, and morusin significantly affect the secretion and production of airway mucin using in vivo and in vitro experimental models. Methods: Effect of AMA was examined on hypersecretion of airway mucin in sulfur dioxide-induced acute bronchitis in rats. Confluent NCI-H292 cells were pretreated with ethanolic extract, kuwanon E, kuwanon G, mulberrofuran G, or morusin for 30 minutes and then stimulated with phorbol 12-myristate 13-acetate (PMA) for 24 hours. The MUC5AC mucin secretion and production were measured by enzyme-linked immunosorbent assay. Results: AMA stimulated the secretion of airway mucin in sulfur dioxide-induced bronchitis rat model; aqueous extract, ethanolic extract, kuwanon E, kuwanon G, mulberrofuran G and morusin inhibited the production of MUC5AC mucin induced by PMA from NCI-H292 cells, respectively. Conclusion: These results suggest that extract of the root bark and the natural products derived from Morus alba L. can regulate the secretion and production of airway mucin and, at least in part, explains the folk use of extract of Morus alba L. as mucoregulators in diverse inflammatory pulmonary diseases.

Keywords

References

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