Journal of the Society of Cosmetic Scientists of Korea (대한화장품학회지)

Society of Cosmetic Scientists of Korea (대한화장품학회)
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Cell migration and Anti-inflammatory Effect of Red Ginseng Extracts Fermented with Laetiporus Sulphureus

붉은덕다리버섯 균사체로 발효한 홍삼 배양액의 cell migration 및 항염 효능에 관한 연구

  • Oh, Seong-Hwa (R&D Center, CoSeedBioPharm Corporation) ;
  • Choi, Soo-Yeon (R&D Center, CoSeedBioPharm Corporation) ;
  • Lee, Nu Rim (R&D Center, CoSeedBioPharm Corporation) ;
  • Lee, Jung No (R&D Center, CoSeedBioPharm Corporation) ;
  • Kim, Dong-Seok (Department of Food Service Industry, Seowon University) ;
  • Lee, Sang-Hwa (Department of Food Service Industry and Bio Organic Material & Food Center, Seowon University) ;
  • Park, Sung-Min (R&D Center, CoSeedBioPharm Corporation)
  • 오성화 ((주)코씨드바이오팜) ;
  • 최수연 ((주)코씨드바이오팜) ;
  • 이누림 ((주)코씨드바이오팜) ;
  • 이정노 ((주)코씨드바이오팜) ;
  • 김동석 (서원대학교 외식산업학과) ;
  • 이상화 (서원대학교 친환경 바이오 소재 및 식품 센터) ;
  • 박성민 ((주)코씨드바이오팜)
  • Received : 2014.06.05
  • Accepted : 2014.08.07
  • Published : 2014.09.30
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Abstract

Red ginseng (RG) contains specific ginsenosides (Rg2, Rg3) which show various pharmacological effects and absorption rate in the body better than panax ginseng. Therefore many people have been used it for health for a long time. Furthermore, many researchers have been studying its biological activities for a long times because fermentation generates lots of beneficial small molecules good for health. In this study, we fermented red ginseng with mycelium of Leatiporus sulphures var. miniatus for 7 days. As a result, we found that three ginsenosides Rg1, Re and Rb2 were decreased from 0.24, 0.25, 0.16 mg/g to 0.12, 0.1, 0.03 mg/g respectively HPLC analysis. In addition, we studied biological activities of fermented red ginseng (FRG) about skin ageing such as anti-inflammation, cell migration, anti-oxidation, collagen type 1 synthesis, and MMP-1 inhibition activities. As a result, FRG were shown higher anti-inflammatory and cell migration promoting activities than RG. FRG inhibited production of nitric oxide (NO) and mRNA expression of inducible nitric oxide synthase (iNOS) and decreased interleukin (IL)-6 induced by LPS stimulation in RAW 264.7 cells. In conclusion, this study suggest that FRG could be a potential source as a new natural anti-inflammatory agent.

홍삼(Red Ginseng; RG)은 인삼보다 더 높은 생체 흡수율과 다양한 약리효과를 갖는 특이한 진세노사이드(Rg2, Rg3)를 함유하고 있다. 따라서 오랫동안 많은 사람들의 건강을 위해 이용되어 왔다. 또한 발효는 유효한 생리활성을 갖는 저분자의 물질들을 생성하기 때문에 많은 연구자들이 생물학적 활성에 대해 오랫동안 연구해오고 있다. 본 연구에서는 홍삼을 붉은덕다리버섯 균사체로 7일 동안 발효하였다. HPLC 분석 결과 진세노사이드 Rg1, Re 및 Rb2가 각각 0.24, 0.25, 0.16 mg/g에서 0.12, 0.1, 0.03 mg/g으로 함량 감소를 확인하였고, 홍삼 붉은덕다리 균사체배양액(Fermented Red Ginseng; FRG)의 항염, 세포 이동, 항산화, 콜라겐 타입 I 합성과 MMP-1 억제효능에 대한 생물학적 효능을 확인하였다. 그 결과, FRG는 RG보다 항염 및 cell migration 촉진효과가 더 우수하였다. FRG는 LPS로 유도된 RAW 264.7 대식세포의 NO 생성을 억제하였으며, iNOS와 IL-6의 발현을 mRNA 수준에서 억제하였다. 이 결과로 FRG는 새로운 항염소재로서 제안이 가능하다고 사료된다.

Keywords

References

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