대한화장품학회지 (Journal of the Society of Cosmetic Scientists of Korea)

  • 제40권3호
  • /
  • Pages.289-295
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  • 2014
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  • 1226-2587(pISSN)
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  • 2288-9507(eISSN)
대한화장품학회 (Society of Cosmetic Scientists of Korea)
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돌외 잎 추출물의 콜라겐 합성 증진 성분 규명

Constituents of Collagen Synthesis Activation from the Extracts of Gynostemma pentaphyllum Leaves

  • 임준환 ((주)내추럴솔루션 중앙연구소) ;
  • 장문식 ((주)내추럴솔루션 중앙연구소) ;
  • 정욱순 ((주)내추럴솔루션 중앙연구소) ;
  • 문미연 ((주)내추럴솔루션 중앙연구소) ;
  • 이하연 ((주)내추럴솔루션 중앙연구소) ;
  • 김영훈 ((주)내추럴솔루션 중앙연구소) ;
  • 이기용 (충북대학교 원예과학과) ;
  • 이남호 (제주대학교 화학.코스메틱스학부)
  • 투고 : 2014.05.02
  • 심사 : 2014.06.03
  • 발행 : 2014.09.30
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초록

본 연구에서는 주름개선 화장품 원료를 개발하기 위하여 돌외 잎 70% 에탄올 추출물을 제조하여 섬유아세포 HDFn에 대하여 콜라겐 합성능을 측정하였다. 그 결과 돌외 추출물이 세포 독성 없이 농도 의존적으로 콜라겐 합성을 촉진함을 확인하였다. 돌외 추출물로부터 용매 분획 및 크로마토그래피 분리과정을 거쳐 2개의 활성 성분을 분리하였다. NMR 데이터 및 문헌치로 확인한 결과 이들은 플라보노이드 배당체인 Ombuine 3-O-rutinoside(1) 및 Quercetin 3-O-rutinoside(2)로 규명되었다. 섬유아세포의 type I procollagen 생합성에 미치는 영향을 분석한 결과 상기 화합물은 농도 의존적으로 콜라겐 합성을 촉진함을 알 수 있었다. 본 실험 결과는 화합물 1과 2를 함유한 돌외 추출물의 주름개선 화장품 소재로서의 개발 가능성을 보여주고 있다.

In order to discover ingredients for wrinkle-care cosmetics, we prepared 70% ethanol extract from Gynostemma pentaphyllum and examined its activity on collagen synthesis using fibroblast HDFn cells. The G. pentaphyllum extract induced the production of type I procollagen in a dose-dependent manner without showing cell toxicity. The active constituents were isolated from the extract by solvent fractionation and chromatographic purification procedures. NMR data and literature studies led to determine the two isolated compounds as the flavonoid glycosides such as ombuine 3-O-rutinoside (1) and quercetin 3-O-rutinoside (2). The activity screening tests showed that the isolates 1 and 2 induced the production of type I procollagen in a dose-dependent manner. These results suggested that G. pentaphyllum extract containing the flavonoids 1 and 2 could be useful as an active ingredient for wrinkle-care cosmetics.

키워드

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피인용 문헌

  1. Development of Pre-assessment Indicator for Skin Aging and Anti-aging Effect of Inner Skin by the Cream Product Containing Gynostemma pentaphyllum Gypenoside Fraction vol.42, pp.3, 2016, https://doi.org/10.15230/SCSK.2016.42.3.303