Journal of the Society of Cosmetic Scientists of Korea (대한화장품학회지)

Society of Cosmetic Scientists of Korea (대한화장품학회)
권호 표지
DOI QR코드

DOI QR Code

Constituents of Collagen Synthesis Activation from the Extracts of Gynostemma pentaphyllum Leaves

돌외 잎 추출물의 콜라겐 합성 증진 성분 규명

  • 임준환 ((주)내추럴솔루션 중앙연구소) ;
  • 장문식 ((주)내추럴솔루션 중앙연구소) ;
  • 정욱순 ((주)내추럴솔루션 중앙연구소) ;
  • 문미연 ((주)내추럴솔루션 중앙연구소) ;
  • 이하연 ((주)내추럴솔루션 중앙연구소) ;
  • 김영훈 ((주)내추럴솔루션 중앙연구소) ;
  • 이기용 (충북대학교 원예과학과) ;
  • 이남호 (제주대학교 화학.코스메틱스학부)
  • Received : 2014.05.02
  • Accepted : 2014.06.03
  • Published : 2014.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

In order to discover ingredients for wrinkle-care cosmetics, we prepared 70% ethanol extract from Gynostemma pentaphyllum and examined its activity on collagen synthesis using fibroblast HDFn cells. The G. pentaphyllum extract induced the production of type I procollagen in a dose-dependent manner without showing cell toxicity. The active constituents were isolated from the extract by solvent fractionation and chromatographic purification procedures. NMR data and literature studies led to determine the two isolated compounds as the flavonoid glycosides such as ombuine 3-O-rutinoside (1) and quercetin 3-O-rutinoside (2). The activity screening tests showed that the isolates 1 and 2 induced the production of type I procollagen in a dose-dependent manner. These results suggested that G. pentaphyllum extract containing the flavonoids 1 and 2 could be useful as an active ingredient for wrinkle-care cosmetics.

본 연구에서는 주름개선 화장품 원료를 개발하기 위하여 돌외 잎 70% 에탄올 추출물을 제조하여 섬유아세포 HDFn에 대하여 콜라겐 합성능을 측정하였다. 그 결과 돌외 추출물이 세포 독성 없이 농도 의존적으로 콜라겐 합성을 촉진함을 확인하였다. 돌외 추출물로부터 용매 분획 및 크로마토그래피 분리과정을 거쳐 2개의 활성 성분을 분리하였다. NMR 데이터 및 문헌치로 확인한 결과 이들은 플라보노이드 배당체인 Ombuine 3-O-rutinoside(1) 및 Quercetin 3-O-rutinoside(2)로 규명되었다. 섬유아세포의 type I procollagen 생합성에 미치는 영향을 분석한 결과 상기 화합물은 농도 의존적으로 콜라겐 합성을 촉진함을 알 수 있었다. 본 실험 결과는 화합물 1과 2를 함유한 돌외 추출물의 주름개선 화장품 소재로서의 개발 가능성을 보여주고 있다.

Keywords

References

  1. L. Maumann, Cosmetic dermatology, principles and practice, 10, McGraw-Hill Co., New York (2002).
  2. S. Shuster, M. M. Black, and E. McVitie, The influence of age and sex on skin thikness, skin collage and density, Br. J. Dermatol., 93, 639 (1975). https://doi.org/10.1111/j.1365-2133.1975.tb05113.x
  3. D. R. Brigstock, The CCN family: A new stimulus package, J. Endocrinol., 178, 169 (2003). https://doi.org/10.1677/joe.0.1780169
  4. G. W. Lee, S. M. Park, Y. C. Yoo, and Y. H. Cho, Effect of ponrici fructus extracts fermented with Ganoderma lucidum on the collagen synthesis and expression of matrix metalloproteinase-1, Korean Soc. Biotechnol. Bioengin. J., 28, 106 (2013).
  5. H. J. Ko, G. B. Kim, D. H. Lee, G. S. Lee, and H. B. Pyo, The effects of hydrolyzed Jeju Ulva pertusa on the proliferation and type I collagen synthesis in replicative senescent fibroblasts, J. Soc. Cosmet. Scientists Korea, 39, 177 (2013). https://doi.org/10.15230/SCSK.2013.39.3.177
  6. V. Razmovski-Naumovski, T. H. W. Huang, V. H. Tran, G. Q. Li, C. C. Duke, and B. D. Roufogalis, Chemistry and pharmacology of Gynostemma pentaphyllum, Phytochem. Reviews, 4, 197 (2005). https://doi.org/10.1007/s11101-005-3754-4
  7. K. Yoshikawa, M. Mitake, T. Takemoto, and S. Arihara, Studies on the constituents of Curcubitaceae plants. XVII. On the saponins constituents of Gynostemma pentaphyllum Makino, Yakugaku. Zasshi., 107, 355 (1987). https://doi.org/10.1248/yakushi1947.107.5_355
  8. Z. P. Fang and X. Y. Zeng, Isolation and identification of flavonoids and organic acid from Gynostemma pentaphyllum Makino, Zhongguo Zhongyao Zazhi, 14, 676 (1989).
  9. Z. Zhang, S. J. Xie, S. P. Huang, and Q. Zhang, Analysis of medicinal and nutritional components in Gynostemma pentaphyllum, Shanxi Daxue Xuebao Ziran Kexueban, 16, 307 (1993).
  10. S. Deng, X. Li, B. Chen, F. Deng, and X. Zhou, Analysis ofamino acids, vitamins, and chemical elements in Gynostemma pentaphyllum (Thunb) Makino, Hunan YikeDaxue Xuebao, 19, 487 (1994).
  11. X. Liu, W. Ye, Z. Mo, B. Yu, S. Zhao, H. Wu, C. Che, R. Jiang, T. C. W. Mak, and W. L. W. Hsaio, Five new ocotillone-type saponins from Gynostemma pentaphyllum, J. Nat. Prod., 67, 1147 (2004). https://doi.org/10.1021/np034018+
  12. D. Arbain, J. R. Cannon, K. Afriastini Kartawinata, R. Djamal, A. Bustari, A. Dharma, H. Rosmawaty Rivai, D. Zaherman Basir, M. Sjafar, R. Sjaiful Nawfa, and S. Kosela, Survey of some west Sumatran plants for alkaloids, Econ. Bot., 43, 73 (1989). https://doi.org/10.1007/BF02859327
  13. Z. J. Wang and D. H. Luo, Antioxidant activities of different fractions of polysaccharide purified from Gynostemma pentaphyllum Makino, Carbohyd. Polymers, 68, 54 (2007). https://doi.org/10.1016/j.carbpol.2006.07.022
  14. T. H. Huang, V. H. Tran, B. D. Roufogalis, and Y. Li, Gypenoside XLIX, a naturally occurring PPAR-alpha activator, inhibits cytokine-induced vascular cell adhesion molecule-1 expression and activity in human endothelial cells, Eur. J. Pharmacol., 565, 158 (2007). https://doi.org/10.1016/j.ejphar.2007.03.013
  15. C. Circosta, R. De Pasquale, and F. Occhiuto, Cardiovascular effects of the aqueous extract of Gynostemma pentaphyllum Makino, Phytomedicine., 12, 638 (2005). https://doi.org/10.1016/j.phymed.2004.06.023
  16. S. Megalli, F. Aktan, N. M. Davies, and B. D. Roufogalis, Phytopreventative anti-hyperlipidemic effects of Gynostemma pentaphyllum in rats, J. Pharm. Pharm. Sci., 8(3), 507 (2005).
  17. J. Hou, S. Liu, Z. MA, X. Lang, J. Wang, J. Wang, and Z. Liang, Effects of Gynostemma pentaphyllum Makino on the immunological functions of cancer patients, J. Trad. Chin. Med., 11, 47 (1991).
  18. A. Norberg, N. K. Hoa, E. Liepinsh, D. Van Phan, N. D. Thuan, H. Jornvall, R. Sillard, and C. G. Ostenson, A novel insulin-releasing substance, phanoside, from the plant Gynostemma pentaphyllum, J. Biol. Chem., 279, 41361 (2004). https://doi.org/10.1074/jbc.M403435200
  19. J. M. Lin, C. C. Lin, H. F. Chiu, J. J. Yang, and S. G. Lee, Evaluation of the anti-inflammatory and liver- protective effects of anoectochilus formosanus, gnoderma lucidum and Gynostemma pentaphyllum in rats, Am. J. Chin. Med., 21, 59 (1993). https://doi.org/10.1142/S0192415X9300008X
  20. C. Rujjanawate, D. Kanjanapothi, and D. Amornlerdpison, The anti-gastric ulcer effect of Gynostemma pentaphyllum Makino, Phytomedicine, 11, 431 (2004). https://doi.org/10.1016/j.phymed.2003.07.001
  21. S. J. Lee, H. J. Bu, J. A. Lee, and D. S. Jung, Screening of plants in Jeju for whitening materials in cosmeceutical, J. Soc. Cosmet. Scientists Korea, 31, 115 (2007).
  22. K. Meada and M. Fukuda, In vitro effectiveness of whitening cosmetic components in human melanocyte, J. Soc. Cosmet. Chem., 42, 361 (1991).
  23. H. Matsuda, T. Morikawa, I. Toquchida, and M. Yoshikawa, Structural requirements of flavonoids and related compounds for aldose reductase inhibitory activity, Chem. Pharm. Bull., 50, 788 (2002). https://doi.org/10.1248/cpb.50.788
  24. K. Kazuma, N. Noda, and M. Suzuki, Malonylated flavonol glycosides from the petals of Clitoria ternatea, Phytochemistry, 62, 229 (2003). https://doi.org/10.1016/S0031-9422(02)00486-7

Cited by

  1. Development of Pre-assessment Indicator for Skin Aging and Anti-aging Effect of Inner Skin by the Cream Product Containing Gynostemma pentaphyllum Gypenoside Fraction vol.42, pp.3, 2016, https://doi.org/10.15230/SCSK.2016.42.3.303