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Establishment of self-specification and shelf-life by standardization of manufacturing process for lyophilized Tenebrio molitor larvae

동결건조 갈색거저리 유충의 제조공정 표준화에 따른 자가규격 및 유통기한 설정

  • 정미연 (농촌진흥청 국립농업과학원 농업생물부) ;
  • 이정용 ((주)월드웨이) ;
  • 이진채 ((주)월드웨이) ;
  • 박길수 ((주)월드웨이) ;
  • 정준표 ((주)월드웨이) ;
  • 황재삼 (농촌진흥청 국립농업과학원 농업생물부) ;
  • 구태원 (농촌진흥청 국립농업과학원 농업생물부) ;
  • 윤은영 (농촌진흥청 국립농업과학원 농업생물부)
  • Received : 2014.04.06
  • Accepted : 2014.06.02
  • Published : 2014.04.30

Abstract

This study was carried out to establish the self-specification and shelf-life by standardization of manufacturing process for Tenebrio molitor larvae. First, standardization of manufacturing process for T. molitor was set up. Sterilization for larvae placed on a multistage shelf with intervals of about 10 cm was carried out at $115^{\circ}C$, $1kgf/cm^2$ for 10 min. After sterilization, T. molitor larvae were frozen at less than $-35^{\circ}C$ for more than 12 hrs. And then, they were dried under $-15^{\circ}C$, 0.5 torr vacuum for more than 30 hrs. Second, we decided self-specification for T. molitor larvae. Their moisture, acidity, peroxide, crude protein and crude fat level should be 5% or less, 3 mg/g or less, 30 meq/g or less, 45% or more, and 25% or more, respectively. Also, oleic acid, representative material, level was set up 11 ~ 16%. Third, we decided shelf-life by analysis of the physicochemical characteristic, sensory evaluation and microbial indicators. The final expiry date for lyophilized T. molitor larvae in PET bottle was calculated as 12 months from date of manufacture. We expect that optimal manufacturing process system, self-specification, and shelf-life proposed in this study can be used in industrial production of T. molitor as a novel food.

본 연구에서는 갈색거저리 유충의 식품등록 후 산업화에 대비하여 대량생산 시설에 적용 가능한 동결건조 갈색거저리 유충곤충의 제조공정을 확립하였다. 갈색거저리 유충을 1 ~ 2일간 절식시킨 후 식용을 목적으로 하므로 위생적인 측면을 고려하여 분변제거 및 세척한 후 고온 고압 살균법으로 살균하였다. 살균시 용기 내에 부직포($48cm{\times}55cm$)를 깔고 갈색거저리 투입량은 1 kg(3 cm)이하로 하는 것이 세균 및 진균의 제거에 효율적임을 확인하였다. 또한 생산효율을 증가시키기 위하여 다단식 살균기를 사용하여 각 단 사이의 공간을 10 cm 이상 확보하여 $115^{\circ}C$, $1kgf/cm^2$에서 10분간 고온 고압 살균한 경우 미생물이 검출되지 않음을 확인하였다. 살균한 갈색거저리 유충 동결건조를 위해 동결은 $-35^{\circ}C$ 이하에서 용기[$W(63cm){\times}L(57cm){\times}H(3cm)$]별 4 kg씩(두께 1.5 cm 이하) 투입하여 실시하고, 건조는 $-15^{\circ}C$ 이하, 0.5 torr 이하에서 최소 30시간 이상 건조하는 것이 효율적인 것으로 판단하였다. 동결건조 갈색거저리 유충의 자가규격으로 수분 5% 이하, 산가 3 mg/g 이하, 과산화물가 30 meq/g 이하, 조단백 45% 이상, 조지방 25% 이상, 올레산 11 ~ 16%를 허용범위로 설정하였다. 마지막으로 이화학적 특성, 관능적 특성, 미생물학적 지표 조사를 통한 유통기한 설정 결과 PET 재질 용기로 포장될 경우 동결건조 갈색거저리 유충의 최종 유통기한은 제조일로부터 12개월로 결정하였다. 따라서 본 연구를 통해 확립된 대량생산 시설에 적용 가능한 동결건조 갈색거저리 유충의 제조공정을 통해 식품등록 후 산업화시 보다 위생적이며 균일한 제품 제조가 가능할 것이고, 자가규격 및 유통기한은 제품 관리에 응용되어 보다 안전성 및 안정성 확보가 가능할 것이라고 사료된다.

Keywords

References

  1. Cha JY, Kim YS, Ahn HY, Eom KE, Park BK, Jun BS, Cho YS (2009) Biological activity of fermented silkworm powder. J Life Sci 19(10), 1468-1477. https://doi.org/10.5352/JLS.2009.19.10.1468
  2. Cho CH, Cha WS, Kim JS (1989) Effect of temperature, time and pH on the extraction of protein in a chrysalis of silkworm. Korean J Biotechnol Bioeng 4, 65-68.
  3. Chung SJ, Lee YH, Chung JH, Lee BR, Han DM (1995) Antifungal effect and activity spectrum of crude antifungal proteins from hemolymph of larvae of Tenebrio molitor in Korea. Kor J Mycol 23(3), 232-237.
  4. Chung MY, Kwon EY, Hwang JS, Goo TW, Yun EY (2013) Pretreatment conditions on the powder of Tenbrio molitor for using as a novel food ingredient. J Seric Entomol Sci 51(1), 9-14.
  5. Ghaly AE (2009) The use of insects as human food in Zambia. OJBS 9(4), 93-104.
  6. Huis A, Vanltterbeeck J, Mertens E, Halloran A, Muir G (2013) Edible insects: future prospects for food and feed security. Food and agriculture organization of the United Nations, Rome.
  7. Kim TS, Lee JH, Choi BD, Byu HS (1987) Nutritional value of dried paddy grasshopper, Oxya chinensis formosana. J Korean Soc Food Nutr 16(2), 98-104.
  8. Lee JH, Kim TS, Choi BD, Kim GE, Lee KH (1987a) Effects of containing pigments of dried grasshopper on the lipid deterioration. J Korean Soc Food Nutr 16, 294-299.
  9. Lee JH, Kim TS, Choi BD, Kim GE, Lee KH (1987b) Effects of containing pigments of dried grasshopper on the lipid deterioration. J Korean Soc Food Nutr 16, 300-305.
  10. MFDS (2011) Food code. Ministry of food and drug safety, Korea.
  11. Park JH, Kim SY, Kang M, Yoon M, Lee YI, Park E (2012) Antioxidant activity and safety evaluation of juice containing Protaetia brevitarsis. J Korean Soc Food Sci Nutr 41(1), 41-48. https://doi.org/10.3746/jkfn.2012.41.1.041
  12. Yoo JM, Hwang JS, Goo TW, Yun EY (2013) Comparative analysis of nutritional and harmful components in korean and chinese mealworms (Tenebrio molitor). J Korean Soc Food Sci Nutr 42(2), 249-254. https://doi.org/10.3746/jkfn.2013.42.2.249

Cited by

  1. Physical and Sensory Evaluation of Tenebrio molitor Larvae Cooked by Various Cooking Methods vol.31, pp.5, 2015, https://doi.org/10.9724/kfcs.2015.31.5.534
  2. Studies on Oxidative Stability of Tenebrio molitor Larvae During Cold Storage vol.31, pp.1, 2015, https://doi.org/10.9724/kfcs.2015.31.1.062