Journal of Sericultural and Entomological Science (한국잠사곤충학회지)

Korean Society of Sericultural Science (한국잠사학회)
권호 표지
DOI QR코드

DOI QR Code

Stability of main components and physiological activities of bee venom treated with pH

산도에 따른 봉독의 성분 및 생리활성에 대한 안정성

  • Cho, Miran (Department of Agricultural Biology, National Academy of Agricultural Science, RDA) ;
  • Han, Sangmi (Department of Agricultural Biology, National Academy of Agricultural Science, RDA) ;
  • Kim, Jungmin (Department of Agricultural Biology, National Academy of Agricultural Science, RDA) ;
  • Yeo, Joohong (Department of Agricultural Biology, National Academy of Agricultural Science, RDA) ;
  • Hong, InPhyo (Department of Agricultural Biology, National Academy of Agricultural Science, RDA) ;
  • Woo, Soonok (Department of Agricultural Biology, National Academy of Agricultural Science, RDA) ;
  • Lee, Kwanggill (Department of Agricultural Biology, National Academy of Agricultural Science, RDA)
  • 조미란 (농촌진흥청 국립농업과학원 농업생물부) ;
  • 한상미 (농촌진흥청 국립농업과학원 농업생물부) ;
  • 김정민 (농촌진흥청 국립농업과학원 농업생물부) ;
  • 여주홍 (농촌진흥청 국립농업과학원 농업생물부) ;
  • 홍인표 (농촌진흥청 국립농업과학원 농업생물부) ;
  • 우순옥 (농촌진흥청 국립농업과학원 농업생물부) ;
  • 이광길 (농촌진흥청 국립농업과학원 농업생물부)
  • Received : 2014.03.19
  • Accepted : 2014.05.02
  • Published : 2014.04.30
Download PDF
⟨ Previous Next ⟩

Abstract

This study was for the investigation of the stability of purified bee venom (PBV) during the treatment in the pH range from pH2 to pH9 for 24 hours, respectively. Changes of components and physiological functionalities in PBV were by evaluated silver staining, and melittin contents were measured by liquid chromatography. The antimicrobial activity against bacteria by minimum inhibitory concentration (MIC) and effect of the cell regeneration were measured by 3-[4,5-dimethyl-2-thiazolyl]-2,5-diphenyl tetrazolium bromide (MTT) assay using human dermal fibroblast (HDF) cell. The main proteins such as melittin and phospholipase $A_2$ showed no characteristic changes. The antimicrobial activity and effect of cell regeneration showed no difference from pH2 to pH9. From this study, we suggest that components and physiological functionalities of PBV against treated pH were kept stability at from pH2 to pH9.

서양종 꿀벌로부터 봉독채집장치를 이용하여 채집한 정제봉독의 pH 안정성에 대하여 평가하고자 멜리틴의 함량, 단백질의변화, 항균력, 세포증식률을 측정하였다. 정제봉독을 pH2에서 pH9까지 24시간 동안 처리한 후 멜리틴 함량과 단백질의 변화, S. aureus, P. acnes, S. mutans와 E. coli에 대한 항균력, 피부세포인 HDF에 대한 세포증식률에 미치는 영향을 분석하였다. pH2에서부터 pH9까지 각각 처리한 정제봉독의 멜리틴 및 단백질 성분의 변화는 확인되지 않았다. 봉독의 주요 약리효과인 항균력에 있어서도 시험균주마다 다소 차이가 있었으나 S. aureus, P. acnes, S. mutans와 E. coli 모두 산도에 따른 유의할 만한 변화는 확인되지 않았다. 또한 피부세포 증식률에 있어서도 산도에 따른 변화는 확인되지 않았다. 이러한 결과로 미루어 봉독의 pH처리는 멜리틴 함량, 단백질의 변화, 항균력 및 세포증식률에 영향을 주지 않는 것으로 확인되어 봉독은 산도의 변화에 있어 안정성이 매우 높은 것으로 사료된다.

Keywords

References

  1. Curcio-Vonlanthen V, Schneider CH, Frutig K, Blaser K, Kalbacher H (1997) Molecular parameters in melittin immunogenicity. J Pept Sci 3(4), 267-276. https://doi.org/10.1002/(SICI)1099-1387(199707)3:4<267::AID-PSC106>3.0.CO;2-7
  2. Fennell JF, Shipman WH, Cole LJ (1968) Antibacterial action of melittin polypeptide from bee venom. Proc Soc Exp Biol Med 127(3), 707-710. https://doi.org/10.3181/00379727-127-32779
  3. Habermann E, Reiz KG (1965) On the biochemisty of bee venom peptides, melittin and apamin. Biochem Z 343(2), 192-203.
  4. Han SM, Lee KG, Yeo JH, Hwang SJ, Jang CH, Chenoweth PJ, Park SC (2009) Effects of bee venom treatment on growth performance of young pigs. Am J Chin Med 37(2), 253-260. https://doi.org/10.1142/S0192415X09006813
  5. Han SM, Lee KG, Yeo JH, Kweon HY, Baek HJ, Park KK (2010) Antibacterial and anti-inflammatory effects of honeybee (Apis mellifera) venom against acce-inducing bacteria. J Med Plat Res 4, 459-464.
  6. Han SM, Lee KG, Yeo JH, Kim WT, Park KK (2011) Biological effects of treatment of an animal skin wound with honeybee (Apis mellifera L.) venom. J Plast Reconstr Aesthet Surg 64(3), e67-72. https://doi.org/10.1016/j.bjps.2010.08.022
  7. Han SM, Lee KG, Yeo JH, Woo SO, Kweon HY (2007) Simplified purificating method of bee venom. Patent 10-0758814.
  8. Kim HW, Kwon YB, Ham TW, Roh DH, Yoon SY, Lee HJ, Han HJ, Yang IS, Beitz AJ, Lee JH (2003) Acupoint stimulation using bee venom attenuates formalin-induced pain behavior and spinal cord fos expression in rabit. J Vet Med Sci 65(3), 349-355. https://doi.org/10.1292/jvms.65.349
  9. Kim MH (1992) Bee venom therapy. pp.112-124. Korea education. Seoul.
  10. Kim MJ, Park SD, Lee AR, Kim KH, Jang JH, Kim KS (2002) The effect of bee venom acupuncture on protease activity and free radical damage in synovial fluid from collagen - induced arthritis in rats. Acupuncture 19, 161-175.
  11. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685. https://doi.org/10.1038/227680a0
  12. Mosmann T (1983) Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 16, 55-63.
  13. Piek T (1986) Venoms of the Hymenoptera. Academic press, London. United Kigdom.
  14. Rudenko SV, Nipot EE (1996) Modulation of melittin-induced hemolysis of erythrocytes. Biokhimiia 61(12), 2116-2124.
  15. Wu M, Hancock RE (1999) Interraction of the cyclic antimicrobial cationic peptide bactenecin with the outer and cytoplasmic membrane. J Biol Chem 274(1), 29-35. https://doi.org/10.1074/jbc.274.1.29

Cited by

  1. 수용액에서 정제봉독 (PBV)과 봉독멜리틴 (BVM)의 안정성 비교 vol.29, pp.4, 2014, https://doi.org/10.5806/ast.2016.29.4.194