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Control Efficacy of Bordeaux Mixture against Pepper Anthracnose

고추 탄저병에 대한 보르도액의 방제 효과

  • Park, Se-Jung (Department of Plant Medicine, College of Agriculture, Life and Environment Science, Chungbuk National University) ;
  • Lee, Soo-Min (Department of Plant Medicine, College of Agriculture, Life and Environment Science, Chungbuk National University) ;
  • Gwon, Hyeon-Wook (Department of Plant Medicine, College of Agriculture, Life and Environment Science, Chungbuk National University) ;
  • Lee, Hotaek (Department of Plant Medicine, College of Agriculture, Life and Environment Science, Chungbuk National University) ;
  • Kim, Heung Tae (Department of Plant Medicine, College of Agriculture, Life and Environment Science, Chungbuk National University)
  • 박세정 (충북대학교 농업생명환경대학 식물의학과) ;
  • 이수민 (충북대학교 농업생명환경대학 식물의학과) ;
  • 권현욱 (충북대학교 농업생명환경대학 식물의학과) ;
  • 이호택 (충북대학교 농업생명환경대학 식물의학과) ;
  • 김흥태 (충북대학교 농업생명환경대학 식물의학과)
  • Received : 2014.06.20
  • Accepted : 2014.07.18
  • Published : 2014.09.30

Abstract

It was conducted to evaluate the control efficacy of Bordeaux mixture on pepper anthracnose in vitro and in vivo. The suppressive efficacy of Bordeaux mixture on mycelial growth and spore germination of Colletotrichum acutatum was investigated on PDA, cellophane membranes and pepper fruits, respectively. Furthermore, its control value was evaluated on detached pepper fruits inoculated with C. acutatum by wound and non-wound inoculation method, and in fields. The mycelial growth of C. acutatum JC24 was inhibited by 90.0% on PDA amended with $937{\mu}g\;mL^{-1}$ of Bordeaux mixture. While the spore germination of C. acutatum JC24 was inhibited perfectly on cellophane membranes treated at $187{\mu}g\;mL^{-1}$ of Bordeaux mixture, that on fruits inoculated with the pathogen by wound inoculation and non-wound inoculation method was inhibited by 88.1% and 91.3%, respectively. Although the control value on fruits treated with $937{\mu}g\;mL^{-1}$ of Bordeaux mixture was 17.6% in wound inoculation method, it was 58.8% in non-wound inoculation method. In fields, when Bordeaux mixture was sprayed five times at 14 day-intervals, it showed 55.7% and 61.7% of control value in 2012 and 2013, respectively. We think Bordeaux mixture was able to use as an eco-friendly organic farming material to control pepper anthracnose based on the above-mentioned results.

고추 탄저병에 대한 보르도액의 방제효과 시험을 in vitro와 in vivo 상태에서 실시하였다. PDA 배지와 셀로판막에서 고추 탄저병균의 균사생장과 포자발아에 대한 억제효과를 조사하였으며, 고추 열매의 표면에서 포자발아와 병 발생에 대한 억제효과를 조사하였다. 또한 2012년과 2013년 2년 동안 고추 포장에서 탄저병 방제 효과를 조사하였다. 탄저병균인 Colletotrichum acutatum JC24는 $4,685{\mu}g\;mL^{-1}$의 보르도액을 첨가한 PDA배지에서 균사생장이 90.0% 억제되었지만, $937{\mu}g\;mL^{-1}$ 처리구에서는 균사생장이 전혀 억제되지 않았다. 셀로판막에서 $187{\mu}g\;mL^{-1}$의 보르도액을 처리하였을 때 C. acutatum JC24의 포자발아가 100% 억제되었고, 고추 열매 상에서는 병원균을 상처 접종하였을 때와 무상처 접종하였을 때, 각각 88.1%와 91.3%가 억제되었다. 고추 열매에 병원균을 무상처 접종하였을 때, $937{\mu}g\;mL^{-1}$의 보르도액 처리구에서 방제가는 58.8%이었지만, 상처 접종을 할 경우에는 효과가 17.6%로 크게 감소하였다. 포장에서도 보르도액은 2012년과 2013년에 55.7%와 61.7%의 효과를 보였다. 이상의 결과를 토대로 보르도액은 친환경농자재로서 C. acutatum에 의한 고추 탄저병을 방제하는데 사용할 수 있을 것으로 생각한다.

Keywords

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