Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
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The Effects of Bee Venom on Tumor Necrosis Factor (TNF)-${\alpha}$ Induced Inflammatory Human HaCaT Keratinocytes

Tumor Necrosis Factor (TNF)-${\alpha}$로 유도된 피부각질형성세포의 염증성 반응에서 봉독의 효과

  • Lee, Woo-Ram (Department of Pathology, Catholic University of Daegu, College of Medicine) ;
  • Kim, Kyung-Hyun (Department of Pathology, Catholic University of Daegu, College of Medicine) ;
  • An, Hyun-Jin (Department of Pathology, Catholic University of Daegu, College of Medicine) ;
  • Kim, Jung-Yeon (Department of Pathology, Catholic University of Daegu, College of Medicine) ;
  • Han, Sang-Mi (Department of Agricultural Biology, National Institute of Agricultural Science and Technology) ;
  • Lee, Kwang-Gill (Department of Agricultural Biology, National Institute of Agricultural Science and Technology) ;
  • Park, Kwan-Kyu (Department of Pathology, Catholic University of Daegu, College of Medicine)
  • 이우람 (대구가톨릭대학교 의과대학 병리학교실) ;
  • 김경현 (대구가톨릭대학교 의과대학 병리학교실) ;
  • 안현진 (대구가톨릭대학교 의과대학 병리학교실) ;
  • 김정연 (대구가톨릭대학교 의과대학 병리학교실) ;
  • 한상미 (농촌진흥청 국립농업과학원) ;
  • 이광길 (농촌진흥청 국립농업과학원) ;
  • 박관규 (대구가톨릭대학교 의과대학 병리학교실)
  • Received : 2014.08.20
  • Accepted : 2014.09.17
  • Published : 2014.09.30
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Abstract

Bee venom (BV) therapy has been used as a traditional medicine to treat a variety of conditions, such as arthritis, back pain, cancerous tumors, and skin diseases. However, regulatory effects of BV on tumor necrosis factor (TNF)-${\alpha}$-induced HaCaT cell migration or anti-inflammatory have not been explored. In the present study, we investigated the effects of BV on HaCaT cell migration and anti-inflammation. HaCaT cell migration was evaluated by wound-healing assay. The pro-inflammatory cytokines such as TNF-${\alpha}$, interleukin (IL)-$1{\beta}$, and IL-8 were examined by ELISA or Western blotting. BV treatment led to an increase in migration of HaCaT cells for 24 and 48 h. Especially, 10 ng/ml of BV were significantly increased HaCaT cell migration. Also, BV suppressed the secretion of TNF-${\alpha}$, IL-$1{\beta}$, and IL-8 in culture medium with HaCaT cells. In addition, Western blot results demonstrate that BV suppressed the expression of TNF-${\alpha}$ and IL-$1{\beta}$, in HaCaT cells. Especially, 1 or 10 ng/ml of BV markedly decreased the expression of pro-inflammatory cytokines. These results demonstrate the potential of BV for the prevention of skin inflammation induced by TNF-${\alpha}$.

Keywords

References

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