The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
권호 표지
DOI QR코드

DOI QR Code

Effects of Calcium, Vitamin D and Egg Yolk Peptide Treatment on the Retardation of Longitudinal Bone Growth Induced by Low-Calcium Diets

저칼슘 식이로 유발한 성장 저하 흰쥐에 대한 칼슘, 비타민 D 및 난황 펩타이드의 투여가 장골 길이 성장에 미치는 영향

  • 김명규 (세명대학교 한의과대학 본초학교실) ;
  • 김혜경 (한서대학교 식품생물공학과) ;
  • 임강현 (세명대학교 한의과대학 본초학교실)
  • Received : 2014.08.19
  • Accepted : 2014.09.18
  • Published : 2014.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

Objectives : Egg yolk is composed of various important chemical substances for human health. A calcium shortage causes the growth retardation on the body growth. In this study, we examined the therapeutic effects of calcium, vitamin D and egg yolk peptide (EYP) treatment on the retardation of the longitudinal bone growth induced by low-calcium diet in adolescent rats. Methods : Low calcium diets were administrated for 15 days. During the last five days, calcium and/or vitamin D and/or EYP were administrated. The body weights, longitudinal bone growth rates, the heights of growth plates, and bone morphogenetic protein (BMP)-2 and insulin-like growth factor (IGF)-1 expressions were measured using histochemical analysis. Results : Low calcium diets caused the significant reduction in body weight gains and the longitudinal bone growth. The heights of growth plates and the expressions of BMP-2 and IGF-1 showed the impairment of body growth as well. Calcium and/or vitamin D administration could not significantly increase the longitudinal bone growth. However, calcium, vitamin D, and EYP administration significantly increased the bone growth, the growth plate height, and BMP-2 and IGF-1 expressions. Conclusions : These results suggest that EYP enhances the longitudinal bone growth in the calcium and/or vitamin D deficiency and it could be a promising agent for the treatment of children suffering from malnutrition.

Keywords

References

  1. Forsyhe RH. Eggs and Egg products as functional ingredients. Bakers Digest. 1970 ; 8 : 4-46.
  2. Korean P. Structure, composition, and formation of egg. poultry research monthly. 2004 ; 174 : 54-7.
  3. Chris Clarke. The science of ice cream. Cambridge. 1st ed. Eng : Royal Society of Chemistry. 2004 : 49.
  4. Heo J. Donguibogam. 1st ed. Seoul : Bubin publishers co. 2005 : 1860.
  5. SATCM. Zhong Hua Ben Cao vol 9. 1st ed. Shanghai : Shanghai Science and Technology Press. 1999 : 477-8.
  6. Hwang KS. Functional properties and commercial application of lecithin. Pusan woman's college theses collection. 1995 ; 17 : 303-19.
  7. Bonjour JP, Benoit V, Rousseau B, Souberbielle JC. Consumption of vitamin D-and calcium-fortified soft white cheese lowers the biochemical marker of bone resorption TRAP 5b in postmenopausal women at moderate risk of osteoporosis fracture. J Nutr. 2012 ; 142 : 698-703. https://doi.org/10.3945/jn.111.153692
  8. Fiorito LM, Mitchell DC, Smiciklas-Wright H, Birch LL. Girls' calcium intake is associated with bone mineral content during middle childhood. J Nutr. 2006 ; 136 : 1281-6. https://doi.org/10.1093/jn/136.5.1281
  9. Matkovic V, Goel PK, Badenhop-Stevens NE, Landoll JD, Li B, Ilich JZ, Skugor M, Nagode LA, Mobley SL, Ha EJ, Hangartner TN, Clairmont A. Calcium supplementation and bone mineral density in females from childhood to young adulthood: a randomized controlled trial. Am J Clin Nutr. 2005 ; 81 : 175-88. https://doi.org/10.1093/ajcn/81.1.175
  10. Li ZZ. Yi Zong Bi Du. 1st ed. Seoul : Tree and earth press. 2000 : 22-4.
  11. Kim DG, Kim YH, Kim JH, Park EJ, Back JH, Lee SH, Lee JY, Jang GT. Dongui pediatry. 1st ed. Seoul : Jungdam press. 2002 : 353, 406-7.
  12. Leem KH, Kim HH. Effects of Herbal Composition on Longitudinal Bone Growth of Adolescent Rats. Kor J Herbology. 2003 ; 18(3) : 181-6.
  13. Catharine R, Christine LT, Ann LY, Heather BDV. Dietary Reference Intakes for Calcium and Vitamin D. 1st ed. Washington, DC : National Academy Press. 2011 : 35-74.
  14. Hollis BW. Assessment of vitamin D nutritional and hormonal status: what to measure and how to do it. Calcif Tissue Int. 1996 ; 58(1) : 4-5. https://doi.org/10.1007/BF02509538
  15. Holick MF, Schnoes HK, DeLuca HF, Suda T, Cousins RJ. Isolation and identification of 1,25-dihydroxycholecalciferol. A metabolite of vitamin D active in intestine. Biochemistry. 1971 ; 10(14) : 2799-804. https://doi.org/10.1021/bi00790a023
  16. Kim HK, Lee S, Leem KH. Protective effect of egg yolk peptide on bone metabolism. Menopause. 2011 ; 18(3) : 307-13. https://doi.org/10.1097/gme.0b013e3181f31b1f
  17. Abdou AM, Watabe K, Yamane T, Isono T, Okamura Y, Kawahito S, Takeshima K, Masuda K, Kim M. Antiosteoporotic effect of orally administered yolk-derived peptides on bone mass in women. Food Sci Nutr. 2014 ; 2(3) : 193-9. https://doi.org/10.1002/fsn3.94
  18. Leem KH, Kim MG, Kim HM, Kim M, Lee YJ, Kim HK. Effects of egg yolk proteins on the longitudinal bone growth of adolescent male rats. Biosci Biotechnol Biochem. 2004 ; 68(11) : 2388-90. https://doi.org/10.1271/bbb.68.2388
  19. Chen H, Hayakawa D, Emura S, Ozawa Y, Okumura T, Shoumura S.Effect of low or high dietary calcium on the morphology of the rat femur. Histol Histopathol. 2002 ; 17(4) : 1129-35.
  20. Choi HJ. New Insight into the Action of Vitamin D. Korean J Fam Med. 2011 ; 32 : 89-96. https://doi.org/10.4082/kjfm.2011.32.2.89
  21. Forriol F, Shapiro F. Bone development: interaction of molecular components and biophysical forces. Clin Orthop Relat Res. 2005 ; 432 : 14-33.
  22. Frank H Netter. Musculoskeletal system: anatomy, physiology, and metabolic disorders. 1st ed. New Jersey : Ciba-Geigy Corporation. 1987 : 130.
  23. Brighton CT, Yoichi S, Robert MH. Cytoplasmic structures of epiphyseal plate chondrocytes; quantitative evaluation using electron micrographs of rat costochondral junctions with specific reference to the fate of hypertrophic cells. J Bone Joint Surg. 1973 ; 55(4) : 771-84. https://doi.org/10.2106/00004623-197355040-00012
  24. Mackie EJ, Ahmed YA, Tatarczuch L, Chen KS, Mirams M. Endochondral ossification: how cartilage is converted into bone in the developing skeleton. Int J Biochem Cell Biol. 2008 ; 40(1) : 46-62. https://doi.org/10.1016/j.biocel.2007.06.009
  25. Gerard JT, Sandra RG. Principles of anatomy & physiolosy. 10th ed. USA : Wiley. 2003 : 174.
  26. Scriver CR, Beaudet AL, Sly WS, Valle D. The Metabolic and Molecular Bases of Inherited Disease. Vol. II. 7th ed. New York : McGraw-Hill. 1995 : 3023-44.
  27. Wang EA, Rosen V, D’Alessandro JS, Bauduy M, Cordes P, Harada T, Israel DI, Hewick RM, Kerns KM, LaPan P, Luxenberg DP, McQuaid D, Moutsatsos IK, Nove J, Wozney JM. Recombinant human bone morphogenetic protein induces bone formation. Proc Nat Acad Sci. 1990 ; 87 : 2220-4. https://doi.org/10.1073/pnas.87.6.2220
  28. Hallahan AR, Pritchard JI, Chandraratna RAS, Ellenbogen RG, Geyer JR, Overland RP, Strand AD, Tapscott SJ, Olson JM. BMP-2 mediates retinoid-induced apoptosis in medulloblastoma cells through a paracrine effect. Nature Med. 2003 ; 9 : 1033-8. https://doi.org/10.1038/nm904
  29. Cancedda R, Descalzi Cancedda F, Castagnola P. Chondrocyte differentiation. Int Rev Cytol. 1995 ; 159 : 265-358. https://doi.org/10.1016/S0074-7696(08)62109-9