The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Isolation and Physiological Functionality of Yeasts from Wild Flowers in Seonyudo of Gogunsanyeoldo, Jeollabuk-do, Korea

전라북도 고군산열도의 선유도 일대 야생화들로부터 효모의 분리 및 생리기능성

  • Hyun, Se-Hee (Department of Biomedicinal Science and Bioechnology, Paichai University) ;
  • Han, Sang-Min (Department of Biomedicinal Science and Bioechnology, Paichai University) ;
  • Lee, Jong-Soo (Department of Biomedicinal Science and Bioechnology, Paichai University)
  • 현세희 (배재대학교 바이오.의생명공학과) ;
  • 한상민 (배재대학교 바이오.의생명공학과) ;
  • 이종수 (배재대학교 바이오.의생명공학과)
  • Received : 2014.09.04
  • Accepted : 2014.09.22
  • Published : 2014.09.30
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Abstract

Sixty one yeast strains of 21 species were isolated from wild flowers in Gogunsanyeoldo, including Seonyudo of Jeollabuk-do, Korea, and identified by comparison of nucleotide sequences for PCR-amplified D1/D2 region of 26S rDNA using BLAST. Among them, Cryptococcus sp. including C. aureus SY1-4 were found to be dominant, and Metschnikowia sp. including M. reukaufii SY20-1 and Rhodotnula sp. such as R. ingeniosa SY1-1 were also abundantly isolated. Some physiological functionalities of the culture broth and cell-free extracts from 61 yeast strains were determined. Supernatant from Metschnilowia reukaufii SY44-6 showed anti-gout xanthine oxidase inhibitory activity of 49.6% and whitening tyrosinase inhibitory activity of 38.4%, respectively.

전라북도 고군산열도 선유도 일대에서 2014년 4월에 개화한 야생화들로부터 효모들을 분리한 후 이들의 26S rDNA의 D1/D2 부위의 염기서열을 결정한 다음 NCBI의 BLAST database에 등록되어 있는 효모들과의 상동성을 비교 분석하여 동정하였다. 야생화 51점에서 21종의 효모들을 61 균주 분리하였고, 이들 중 Cryptococcus aureus SY1-4 등의 Cryptococcus 속 균들이 가장 많았고, Metschnikowia reukaufii SY20-1등 Metschnikowia 속 균과 Rhodotorula ingeniosa SY1-1등 Rhodotorula 속 균들도 비교적 많이 분리되었다. 이들 61 균주들의 배양상등액과 무세포추출물을 제조한 후 생리기능성을 측정한 결과 M. reukaufii SY44-6의 배양상등액이 각각 49.6%의 XOD 저해활성과 38.4%의 미백성 Tyrosinase 저해활성을 보여 우수하였다.

Keywords

References

  1. Kwon SC, Jeon TW, Park JS, Kwak JS, Kim TY. Inhibitory effect on tyrosinase, ACE and xanthine oxidase, and nitrite scavenging activities of Jubak (Alcohol filter cake) extracts. J Kor Soc Food Sci Nutr 2012;41:1191-6. https://doi.org/10.3746/jkfn.2012.41.9.1191
  2. Min JH, Kim YH, Kim JH, Choi SY, Lee JS, Kim HK. Comparison of microbial diversity of Korean commercial Makgeolli showing high ${\beta}$-glucan content and high antihypertensive activity, respectively. Mycobiology 2012;40:138-41. https://doi.org/10.5941/MYCO.2012.40.2.138
  3. Lee DH, Lee DH, Lee JS. Characterization of a new antidementia ${\beta}$-secretase inhibitory peptide from Saccharomyces cerevisiae. Enzyme Mucrib Tech 2007;42:83-8. https://doi.org/10.1016/j.enzmictec.2007.08.003
  4. Lee JS, Yi SH, Kwon SJ, Ahn C, Yoo JY. Isolation identification and cultural conditions of yeasts from traditional meju. Kor J Appl Microbiol Biotechnol 1997;25:435-41.
  5. Min JH, Hyun SH, Kang MG, Lee HB, Kim CM, Kim HK, Lee JS. Isolation and identification of yeasts from wild flowers of Daejeon city and Chungcheongnam-do in Korea. Kor J Mycol 2012;40:141-4. https://doi.org/10.4489/KJM.2012.40.3.141
  6. Kang MG, Hyun SH, Ryu JJ, Min JH, Kim HK, Lee JS. Note on newly isolated yeasts from wild flowers in Daejeon city, Korea. Kor J Mycol 2012;40:174-6. https://doi.org/10.4489/KJM.2012.40.3.174
  7. Min JH, Ryu JJ, Kim HK, Lee JS. Isolation and identification of yeasts from wild flowers in Gyejoksan, Oseosan and Beakamsan of Korea. Kor J Mycol 2013;41:47-51. https://doi.org/10.4489/KJM.2013.41.1.47
  8. Min JH, Lee HB, Lee JS, Kim HK. Identification of yeasts isolated from wild flowers collected in coast areas of Korea based on the 26S rDNA sequences. Kor J Mycol 2013;41:185-91. https://doi.org/10.4489/KJM.2013.41.3.185
  9. Hyun SH, Mun HY, Lee HB, Kim HK, Lee JS. Isolation of yeasts from wild flowers in Gyonggi-do and Jeju island of Korea and production of anti-gout xanthine oxidase inhibitor. J Microbiol Biotechnol 2013;41:383-90. https://doi.org/10.4014/kjmb.1309.09002
  10. Hyun SH, Min JH, Lee HB, Kim HK, Lee JS. Isolation and diversity of yeasts from wild flowers in Ulleungdo and Yokjido, Korea. Kor J Mycol 2014;42:28-33. https://doi.org/10.4489/KJM.2014.42.1.28
  11. Hyun SH, Lee JG, Park WJ, Kim HK, Lee JS. Isolation and diversity of yeasts from fruits and flowers of orchard in Sinam-myeon of Yesan-gun, Chungcheongnam-do, Korea. Kor J Mycol 2014;42:21-7. https://doi.org/10.4489/KJM.2014.42.1.21
  12. Hyun SH, Min JH, Kim SA, Kim HK, Lee JS. Yeasts associated with fruits and blossoms collected from Hanbat arboretum, Daejeon, Korea. Kor J Mycol 2014;42:178-82. https://doi.org/10.4489/KJM.2014.42.2.178
  13. Blois MS. Antioxidant determination by the use of stable free radical. Nature 1958;181:1199-200. https://doi.org/10.1038/1811199a0
  14. Kim HK, Lee EN, Geum JH, Lee JS. Nutritional characteristics and physiological functionality of ployplant extracts from some vegetables and medicinal plants. Kor J Food Nutr 2008;21:410-5.
  15. Jang IT, Kim YH, Kang MG, Lee SH, Lim SI, Lee JS. Production of tyrosinase inhibitor from Saccharomyces cerevisiae. Kor J Mycol 2012;40:60-4. https://doi.org/10.4489/KJM.2012.40.1.060
  16. Hyun KW, Kim JH, Song KJ, Lee JB, Jang JH, Kim YS, Lee JS. Physiological functionality in Geumsan perilla leaves from greenhouse and field cultivation. Kor J Food Sci Technol 2003;35:975-9.
  17. Chan M, Lye D, Win MK, Chow A, Barkham T. Clinical and microbiological characteristics of cryptococcosis in Singapore: predominance of Cryptococcus neoformans compared with Cryptococcus gattii. Int J Infect Dis 2014;26:110-5. https://doi.org/10.1016/j.ijid.2014.05.019
  18. Nozaki H, Suzuki S, Tsuyoshi N, Yokozeki K. Production of D-arabitol by Metschnikowia reukaufii AJ14787. Biosci Biotechnol Biochem 2003;67:1923-9. https://doi.org/10.1271/bbb.67.1923
  19. Zhang Z, Zhang X, Tan T. Lipid and carotenoid production by Rhodotorula glutinis under irradiation/high-temperature and dark/low-temperature cultivation. Bioresour Technol 2014;157:149-53. https://doi.org/10.1016/j.biortech.2014.01.039
  20. Seo GW, Cho JY, Kuk JH, Wee JH, Moon JH, Kim SH, Park KH. Identification of antioxidative substances in Allium fistulosum L. by GC-MS. Kor J Food Sci Technol 2003;35:988-93.
  21. Kang YH, Park YK, Oh SR, Moon KD. Studies on the physiological functionality of fine needle and mugwort extracts. Kor J Food Sci Technol 1995;27:978-84.

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