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Simplified the Screening and In Vitro Appraisal of Antioxidant, Cytotoxic, Thrombolytic, Antimicrobial and Membrane Stabilizing Activities of Lablab Purpures at a Time

  • Rahman, M. Saifur (Department of Pharmacy, Chosun University) ;
  • Uddin, M. Gias (Department of Biology, New York University) ;
  • Alam, M. Badrul (Department of Biotechnology, Kyungpook National University) ;
  • Yoo, Jin Cheol (Department of Pharmacy, Chosun University)
  • 투고 : 2014.09.12
  • 심사 : 2014.09.25
  • 발행 : 2014.09.30

초록

To simplify the different biological investigation of the methanolic extract and solvent-solvent partitioning of Lablab purpures (L. purpures) bark. In-vitro anti-oxidant study was determined using total DPPH radical scavenging assay. In vitro antimicrobial study was measured by observing zone of inhibition. The cytotoxic activity was studied using brine shrimp lethality bioassay and thrombolytic activity by clot disruption method. The antioxidant potential was evaluated by 1,1-diphenyl-2-picrylhydrazyl (DPPH) and Folin-Ciocalteau reagents using butylated hydroxytolune (BHT) and ascorbic acid as standards. The Aqueous soluble fraction revealed the highest free radical scavenging activity ($IC_{50}=48.76{\mu}g/mL$). The antimicrobial screening of the bark of L. purpures exhibited mild to moderate activity in test microorganisms. The CSF showed the maximum relative percentage inhibition against Salmonella parathyphi (34.2%) for bacteria and C. albicans (28.8%) for fungi whereas, lowest relative percentage inhibition against Sarcina lutea (22.0%) for bacteria and Aspergillus niger (24.4%) for fungi. In the brine shrimp lethality bioassay, The $LC_{50}$ values of Carbon tetrachloride and N-Hexane soluble fraction were found $92.18{\mu}g/mL$, and $68.95{\mu}g/mL$ respectively while the $LC_{50}$ values of standard Vincristine sulphate was $1.37{\mu}g/mL$. The methanolic extract and its organic soluble fractions of Lablab purpureus at concentration 2.0 mg/mL, significantly protected the lysis of erythrocyte membrane induced by hypotonic solution and heat as compared to the standard, acetyl salicylic acid (0.10 mg/mL). The MSF and AQSF produced 61.48 % and 53.75% inhibition of hemolysis of RBC caused by hypotonic solution respectively, whereas acetyl salicylic acid (0.10 mg/mL) showed 76.42%. Ethanol extract of L. purpures and all of its different partitions exhibited moderate thrombolytic activity of 37.25%-2.40%. Very good preliminary screening and simplified experiments were able to show the different biological activity of methanolic extract and its soluble fractions of L. purpures at a time.

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참고문헌

  1. N. R. Fransworth, "Ethnopharmacology and future drug development: the North American experience", J. Ethnopharmacol., Vol. 38, pp. 45-152, 1993.
  2. P. J. Houghton, "The role of plants in traditional medicine and current therapy", J. Altern. Complement Med., Vol. 1, pp. 131-143, 1995. https://doi.org/10.1089/acm.1995.1.131
  3. D. J. Minson and J. R. Wilson, "Comparative digestibility of tropical and temperate forage-a contrast between grasses and legumes", J. Aust. Inst. Agric. Sci., Vol. 46, pp. 247-249, 1980.
  4. M. N. McLeod and D. J. Minson, "The analytical and biological accuracy of estimating the dry matter digestibility of different legume species", Anim. Feed Sci. Technol., Vol. 1, pp. 61-72, 1976. https://doi.org/10.1016/0377-8401(76)90008-0
  5. R. E. Hendricksen, D. P. Poppi, and D. J. Minson, "The voluntary intake, digestibility and retention time by cattle and sheep of stem and leaf fractions of a tropical legume (Lablab purpureus)", Aust J. of Agric. Res., Vol. 32, pp. 389-398, 1981. https://doi.org/10.1071/AR9810389
  6. R. E. Hendricksen and D. J. Minson, "The feed intake and grazing behaviour of cattle grazing a crop of Lablab purpureus cv. Rongai", J. Agric. Sci., Vol. 95, pp. 547-554, 1980. https://doi.org/10.1017/S0021859600087955
  7. M. Rahmatullah, M.A.H. Mollik, A.K. Paul, R. Jahan, M.A. Khatun, S. Seraj, A.R. Chowdhury, A. Bashar, R. Wahab, and M. Taufiq-ur-Rahman, "A comparative analysis of medicinal plants used to treat gastrointestinal disorders in two sub-districts of Greater Khulna Division, Bangladesh", Adv. Nat. Appl. Sci., Vol. 4, pp. 22-28, 2010.
  8. P. Rastogi and B. N. Mehrotra, "Compendium of Indian medicinal plants, drug research perspective", CDRI Lucknow and NISCOM, New Delhi., Vol. 6, pp. 1-859, 1999.
  9. B. Alam, F. Akter, N. Parvin, R. S. Pia, S. Akter, J. Chowdhury, K. S. E. Jahan, and E. Haque, "Antioxidant, analgesic and anti-inflammatory activities of the methanolic extract of Piper betle leaves", Avicenna J. Phytomed., Vol. 3, pp.112-125, 2013.
  10. A. Braca, N. D. Tommasi, L. D. Bari, C. Pizza, M. Politi, and I. Morelli, "Antioxidant principles from Bauhinia terapotensis", J. Nat. Prod., Vol. 64, pp. 892-895, 2001. https://doi.org/10.1021/np0100845
  11. S. Prasad, R. S. Kashyap, J. Y. Deopujari, H. J. Purohit, G. M. Taori, and H. F. Daginawala, "Development of an in vitro model to study clot lysis activity of thrombolytic drugs", Thromb. J., Vol. 4, pp. 1-4, 2006.
  12. B. N. Meyer, N. R. Ferrigni, J. E. Putnam, J. B. Jacobsen, D. E. Nicholsand, and J.L. Mclaughlin, "Brine shrimp; a convenient general bioassay for active plant constituents", Planta Med., Vol. 45, pp. 31-34, 1982. https://doi.org/10.1055/s-2007-971236
  13. P. R. Murray, E. J. Baron, M. A. Pfaller, and F. C. Tenover, "Manual of clinical microbiology", 6th Edn., ASM Press, Washington DC, pp. 15, 1995.
  14. N. S. Chowdhury, M. B. Alam, A. Haque, R. Zahan, M. Mazumder, and E. Haque, "In vitro free radical scavenging and thrombolytic activities of Bangladeshi aquatic plant Aponogeton undulates Roxob", Global J. Pharmacol., Vol. 5, pp. 27-32, 2011.
  15. K. K. Ajay, R. M. K. Lokanatha, and K. B. Umesha, "Evaluation of antibacterial activity of 3, 5-dicyano-4,6-diaryl-4-ethoxycarbonyl-piperid-2-ones", J. Pharm. Biomed. Anal., Vol. 27, pp. 837-840, 2002. https://doi.org/10.1016/S0731-7085(01)00456-3
  16. U. A. Shinde, A. S. Phadke, A. M. Nair, A. A. Mungantiwar, V. J. Dikshit, and M. N. Saraf, "Membrane stabilizing activity-a possible mechanism of action for the anti-inflammatory activity of Cedrus deodara wood oil", Fitoterapia, Vol. 70, pp. 251-257, 1999. https://doi.org/10.1016/S0367-326X(99)00030-1
  17. T. Nakayama, "Suppression of hydroperoxideinduced cytotoxicity by polyphenols", Cancer Res., Vol. 54, pp. 1991s-1993s, 1994.
  18. C. R. M. Prentice, "Platelets and atherosclerosis", Eur. Heart J. Suppl., Vol. 1, pp. A3-A7. 1999.
  19. A. Banerjee, Y. Chisti, and U. C. Banerjee, "Streptokinase-a clinically useful thrombolytic agent research review paper", Biotechnol Adv., Vol. 22, pp. 287-307, 2004. https://doi.org/10.1016/j.biotechadv.2003.09.004
  20. S. Prasad, R. S. Kashyap, J. Y. Deopujari, H. J. Purohit, G. M. Taori and H. F. Daginawala, "Effect of Fagonia Arabica (Dhamasa) on in vitro thrombolysis", BMC Comp. Alter. Med., Vol. 7, pp. 1-6, https://doi.org/10.1186/1472-6882-7-1
  21. S. Priya and M. Karthika, "Antibacterial activity of the volatile oil of lablab purpureus against some burn isolates", Int. J. Pharm. Sci. Res., Vol. 4, pp. 54-56, 2013. https://doi.org/10.7897/2230-8407.04612
  22. J. E. Middleton, "Biological properties of plant flavonoids: an overview", Pharm. Biol., Vol. 34, pp. 344-348, 1996. https://doi.org/10.1076/phbi.34.5.344.13245
  23. J. Sadique, W. A. Al-Rqobah, M. E. Bugharlth, and A. R. El Gindy, "The bioactivity of certain medicinal plants on the stabilization of RBC membrane system", Fitoterapia, Vol. 60, pp. 525-532, 1989.
  24. E. Souri, G. Amin, H. Farsam, and M. Barazandeh Tehrani, "Screening of antioxidant activity and phenolic content of 24 medicinal plant extracts", Daru, Vol. 16, pp. 83-87, 2008.
  25. O. A. El Shanbrany, O. D. El Gindi, F. R. Melek, S. M. Abdel Khalk, and M. Y. Haggig, "Biological properties of saponin mixtures of Fagonia cretica and Fagonia mollis", Fitoterapia, Vol. 48, pp. 219-222, 1977.
  26. O. O. Oyedapo, "Biological activity of Plyllanthus amarus extracts on pragrow-Dawley rats", Nig. J. Biochem. Mol. Biol., Vol. 16, pp. 83-86, 2001.