Journal of The Korean Society of Grassland and Forage Science (한국초지조사료학회지)

The Korean Society of Grassland and Forage Science (한국초지조사료학회)
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Enhancement of Nutritional Quality of Italian Ryegrass Mediated Silage by Supplemented with Lactic Acid Bacteria and Chlorella

  • Vijayakumar, Mayakrishnan (Grassland and Forage Division, National Institute of Animal Science, Rural Development Administration) ;
  • Ilavenil, Soundarrajan (Grassland and Forage Division, National Institute of Animal Science, Rural Development Administration) ;
  • Arasu, Mariadhas Valan (Grassland and Forage Division, National Institute of Animal Science, Rural Development Administration) ;
  • Jung, Min-Woong (Grassland and Forage Division, National Institute of Animal Science, Rural Development Administration) ;
  • Park, Hyung Soo (Grassland and Forage Division, National Institute of Animal Science, Rural Development Administration) ;
  • Kim, Ji Hea (Grassland and Forage Division, National Institute of Animal Science, Rural Development Administration) ;
  • Lim, Young Cheol (Grassland and Forage Division, National Institute of Animal Science, Rural Development Administration) ;
  • Choi, Ki Choon (Grassland and Forage Division, National Institute of Animal Science, Rural Development Administration)
  • Received : 2014.08.13
  • Accepted : 2014.09.19
  • Published : 2014.09.30
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Abstract

The aim of present study was to improve the quality of silage using lactic acid bacteria (LAB) and chlorella as a supplement. Italian ryegrass (IRG) mediated silage was prepared with lactic acid bacteria (L. plantarum) and different concentration of chlorella. We analyzed the nutritional profiles such as crude protein (CP), acid detergent fiber (ADF) neutral detergent fiber (NDF), total digestible nutrient (TDN) and in-vitro dry matter digestibility (IVDMD), microbial counts and fermentative acids such as lactic acid, acetic acid and butyric acid in the control and experimental silage after three months. It shows increased crude protein content and also maintains the rest of nutritional values as compared with control silage. LAB inoculation with chlorella as supplementation slightly reduced the pH of the silage. In addition, it increased the fermentative acids production as compared with control silage and inhibits the undesired microbial growth especially fungi in the silage. Therefore, we suggest that LAB inoculation and chlorella supplementation to the IRG mediated silage could be improved the nutritional quality of the silage which is an intrinsic feature for the application in the preparation of animal feeds and functional foods.

Keywords

References

  1. AOAC. 1990. Official methods of analysis (15th ed.). Association of analytical chemisits, Washington, DC.
  2. Chisti, Y. 2007. Biodiesel from microalgae. Biotechnology Advances. 25:294-306. https://doi.org/10.1016/j.biotechadv.2007.02.001
  3. Ferreira, D.A., Goncalves, L.C., Molina, L.R., Castro Neto, A.G. and Tomich, T.R. 2007. Caracteristicas de fermentacao da silagem de cana-de-acucar tratada com ureia, zeolita, inoculante bacteriano e inoculante bacteriano/enzimatico. Arquivo Brasileiro de Medicina Veterinaria e Zootecnia. 59:423-433. https://doi.org/10.1590/S0102-09352007000200024
  4. Kristensen, N.B., Storm, A., Raun, B.M.L., Rojen, B.A. and Harmon, D.L. 2007. Metabolism of silage alcohols in lactating dairy cows. Journal of Dairy Science. 90:1364-1377. https://doi.org/10.3168/jds.S0022-0302(07)71623-5
  5. Mata, T.M., Cardoso, N., Ornelas, M., Neves, S. and Caetano, N.S., 2010. Sustainable production of biodiesel from tallow, lard and poultry fat and its quality evaluation. Chemical Engineering Transactions. 19:13-18.
  6. McDonald, P., Henderson, A.R. and Heron, S.J.E. 1991a. The Biochem istry of Silage. Chalcombe Publications, Marlow, Buckinghamshire, Bucks, UK.
  7. McDonald, P.J., Henderson, A.R., Heron, S.J.E. 1991b. The biochemistry of silage ($2^a$ Ed.) Mallow Chalcombe publications, ISBN 0948617225.
  8. Miller, T.L. and Wolin, M.J. 1974. A serum bottle modification of the Hungate technique for cultivating obligate anaerobes. Applied Microbiol. 27:985-987.
  9. Moore, J.E. 1970. Procedure for the two-stage in vitro digestion of forage. Univ. of Florida, Department of Animal Science.
  10. Oude, E.S.J.W.H., Driehuis, F., Gottschal, J.C. and Spoilstra, S.F. 2000. Silage fermentation processes and their manipulation. FAO Electronic Conference on Tropical Silage. 1-28.
  11. Van Soest, P.J. 1993. Cell wall matrix interactions and degradation--session synopsis. 3 In H.G. Jung, D.R. Buxton, R.D. Hatfield, and J. Ralph eds. Forage Cell Wall Structure and Digestibility. American Soc. Agronomy, Madison, W. 77-395.
  12. Weinberg, Z.G. and Muck, R.E. 1996. New trends and opportunities in the development and use of inoculants for silage. FEMS Microbiological Review. 19:53-68.
  13. Woolford, M.K. 1984. The silage fermentation. Marcel Dekker, ISBN 0824770390, New York.
  14. Weinberg, Z.G. 2003. Effect of Lactic acid Bacteria on animal performance. Indian Journal of Biotechnology. 2:378-381

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