Journal of Sensor Science and Technology (센서학회지)

The Korean Sensors Society (한국센서학회)
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Glucose Sensors Using Lipoic Acid Self-Assembled Monolayers

  • Kim, Ji Yeong (Department of Materials Science and Engineering, Inha University) ;
  • Nakayama, Tadachika (Extreme Energy-Density Research Institute, Nagaoka University of Technology) ;
  • Kim, Jae-Hun (Department of Materials Science and Engineering, Inha University) ;
  • Kim, Sang Sub (Department of Materials Science and Engineering, Inha University)
  • Received : 2014.07.03
  • Accepted : 2014.09.22
  • Published : 2014.09.30
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Abstract

A novel approach to fabricating high-performance glucose sensors is reported, which is based on the process of self-assembled monolayers (SAMs). In this study, we have particularly used ${\alpha}$-lipoic acid (LA) SAMs for the glucose sensors. To our best knowledge, this study is the first one to use LA as SAMs for this purpose. N-hydroxysuccinimide (NHS) and 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) were deliberately attached at the same time on the LA SAM. Then, glucose oxidase ($GO_X$) and horseradish peroxidase (HRP) were sequentially immobilized. Thus, the HRP/$GO_X$/NHS-EDC/LA-SAM/Au/Cr/glass working electrode was developed. The glucose-sensing capability of the fabricated sensor was systematically measured by the use of cyclic voltammetry in the range of 1-30 mM glucose in phosphate-buffered saline. The result showed a good sensitivity, that is, as high as $27.5{\mu}A/(mM{\cdot}cm^2)$. This result conspicuously demonstrates that LA can be one of promising substances for use as SAMs for accurately monitoring trace levels of glucose concentration in human blood.

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