Asian Pacific Journal of Cancer Prevention

Asian Pacific Journal of Cancer Prevention (아시아태평양암예방학회)
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Development of In-House Multiplex Real Time PCR for Human Papillomavirus Genotyping in Iranian Women with Cervical Cancer and Cervical Intraepithelial Neoplasia

  • Sohrabi, Amir (Department of Molecular Medicine, School of Advanced Medical Technologies, Tehran University of Medical Sciences) ;
  • Mirab-Samiee, Siamak (Food and Drug Laboratory Research Center, Ministry of Health and Medical Education) ;
  • Modarressi, Mohammad Hossein (Department of Medical Genetics, School of Medicine, Tehran University of Medical Sciences) ;
  • Izadimood, Narge (Department of Pathology, Tehran Women Central Hospital) ;
  • Azadmanesh, Kayhan (Department of Virology, Pasteur Institute of Iran) ;
  • Rahnamaye-Farzami, Marjan (Research Center of Health Reference Laboratory, Ministry of Health and Medical Education)
  • Published : 2014.08.15
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Abstract

Background: HPV related cervical cancer as one of the most common women cancers in developing countries. Regarding accessibility of commercial vaccines, any long or short term modality for integrating preventive immunization against HPV in a national program needs comprehensive information about HPV prevalence and its genotypes. The important role of selecting most accurate diagnostic technologies for obtaining relevant data is underlined by different assays proposed in the literature. The main objective of the present study was to introduce an in-house HPV typing assay using multiplex real time PCR with reliable results and affordable cost for molecular epidemiology surveys and diagnosis. MATERIALS AND METHODS: 112 samples of formalin fixed paraffin embedded tissues and liquid based cytology specimens from patients with known different grades of cervical dysplasia and invasive cancer, were examined by this method and the result were verified by WHO HPV LabNet proficiency program in 2013. RESULTS: HPV was detected in 105 (93.7%) out of 112 samples. The dominant types were HPV 18 (61.6%) and HPV 16 (42.9%). Among the mixed genotypes, HPV 16 and 18 in combination were seen in 12.4% of specimens. CONCLUSIONS: According to acceptable performance, easy access to primers, probes and other consumables, affordable cost per test, this method can be used as a diagnostic assay in molecular laboratories and for further planning of cervical carcinoma prevention programs.

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References

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