Journal of Food Hygiene and Safety (한국식품위생안전성학회지)

The Korean Society of Food Hygiene and Safety (한국식품위생안전성학회)
권호 표지
DOI QR코드

DOI QR Code

Bactericidal Efficacy of a Disinfectant Solution Composed to Povidine-iodine Against Salmonella typhimurium and Brucella ovis

  • Park, Eun-Kee (Department of Medical Humanities and Social Medicine, College of Medicine, Kosin University) ;
  • Cho, Youyoung (Department of Nursing, Hanyeong College) ;
  • Lee, Hu-Jang (Research Institute of Live Sciences, College of Veterinary Medicine, Gyeongsang National University)
  • Received : 2014.05.13
  • Accepted : 2014.09.01
  • Published : 2014.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

Salmonella spp. and Brucella spp. are associated with considerable diseases of both humans and animals. In addition, these microorganisms cause the economic loss in animal farming and food industry. In this study, the disinfection efficacy of a commercial disinfectant, composed to povidone-iodine was evaluated against S. typhimurium and B. ovis. A bactericidal efficacy test by broth dilution method was used to determine the lowest effective dilution of the disinfectant following exposure to test bacteria for 30 min at $4^{\circ}C$. The disinfectant and test bacteria were diluted with hard water (HW) or organic matter suspension (OM) according to treatment condition. On HW condition, the bactericidal activity of the disinfectant against S. typhimurium and B. ovis was 400 and 150 fold dilutions, respectively. On OM condition, the bactericidal activity of the disinfectant was 5 and 20 fold dilutions against S. typhimurium and B. ovis, respectively. As the disinfectant composed to povidine-iodine possesses bactericidal efficacy against animal pathogenic bacteria such as S. typhimurium and B. ovis, the disinfectant solution can be used to control the spread of bacterial diseases.

살모넬라증과 부루셀라증은 가축에 심각한 피해를 유발하는 질병으로, 축산업과 식품산업에 많은 경제적 손실을 초래하고 있다. 본 연구에서는, 포비돈-아이오딘을 주성분으로 하는 소독제 베타딘$^{(R)}$ 농후액의 Salmonella typhimurium과 Brucella ovis에 대한 효력시험을 수행하였다. 배지희석법을 이용한 살균효력시험은 $4^{\circ}C$에서 30분 동안 시험 세균을 희석 소독제에 노출시켜 소독제의 가장 효과적인 낮은 희석배수를 결정하는 시험이다. 베타딘$^{(R)}$ 농후액과 시험 세균들을 처리조건에 따라 경수와 유기물로 희석하여 반응을 시켰다. 유기물 조건에서, Salmonella typhimurium과 Brucella ovis에 대한 베타딘$^{(R)}$ 농후액의 살균력은 경수조건에서의 살균력과 비교하여 낮게 나타났는데, 이는 유기물들에 의한 소독제의 살균 유효성분에 대한 저해작용에 따른 것으로 사료된다. 베타딘$^{(R)}$ 농후액은 Salmonella typhimurium과 Brucella ovis와 같은 가축병원성 질병들에 대해 살균효과를 가지므로, 살모넬라증과 브루셀라증과 같은 세균성 질병의 확산을 제어하는데 효과적으로 이용될 수 있을 것으로 사료된다.

Keywords

References

  1. Cohen, H.J., Mechanda, S.M. and Lin, W.: PCR amplification of the fimA gene sequence of Salmonella typhimurium, a specific method for detection of Salmonella spp. Appl. Environ. Microbiol. 62, 4303-4308 (1996).
  2. Cleaveland, S., Laurenson, M.K. and Taylor. L.H.: Diseases of humans and their domestic mammals: pathogen characteristics, host range and the risk of emergence. B. Biol. Sci. 356, 991-999 (2001). https://doi.org/10.1098/rstb.2001.0889
  3. Kim, G.S., Kim, D.H., Lim, J.J., Lee, J.J., Han, D.Y., Lee, W.M., Jung, W.C., Min, W.G., Won, C.G., Rhee, M.H., Lee, H.J. and Kim, S.: Biological and antibacterial activities of the natural herb Houttuynia cordata water extract against the intracellular bacterial pathogen Salmonella within the raw 64.7 macrophage. Biol. Pharm. Bull. 31, 2012-2017 (2009).
  4. Kim, D.H., Lim, J.J., Lee, J.J., Jung, W.C., Shin, H.J., Lee, H.J., Kim, G.S. and Kim, S.: Antibacterial and therapeutic effects of Houttuynia cordata ethanol extract for murine salmonellosis. Kor. J. Environ. Agricul. 27, 156-162 (2008). https://doi.org/10.5338/KJEA.2008.27.2.156
  5. Ibrahim, M.A., Emeash, H.H., Ghoneim, N.H. and Abdel- Halim, M.A.: Seroepidemiological studies on poultry salmonellosis and its public health importance. J. World's Poult. Res. 3, 18-23 (2013).
  6. Katsuda, K., Kohmoto, M., Kawashima, K. and Tsunemitsu, H.: Frequency of enteropathogen detechtion in sucking and weaned pigs with diarrhea in Japan. J. Vet. Diagn. Invest. 18, 350-354 (2006). https://doi.org/10.1177/104063870601800405
  7. Korsak, N., Jacob, B., Groven, B., Etienne, G., China, B., Ghafir, Y. and Daube, G.: Salmonella contamination of pigs and pork in an integrated pig production system. J. Food Prot. 66, 1126-1133 (2003). https://doi.org/10.4315/0362-028X-66.7.1126
  8. Hubalek, Z. and Rudolf, I.: Microbial zoonoses and sapronoses. Springer, Heidelberg, pp. 214-215 (2011).
  9. Moreno, E., Cloechkaert, A. and Morivon, I.: Brucella evolution and taxonomy. Vet. Microbiol. 90, 209-227 (2002). https://doi.org/10.1016/S0378-1135(02)00210-9
  10. Cha, C.N., Lee, Y.E., Kang, I.J., Yoo, C.Y, An, S., Kim, S. and Lee, H.J.: Bactericidal efficacy of Vital-$Oxide^{(R)}$, disinfectant solution against Salmonella Typhimurium and Brucella ovis. J. Fd Hyg. Safety, 27, 50-54 (2012). https://doi.org/10.13103/JFHS.2012.27.1.050
  11. Cloeckaert, A., Grayon, M., Gr pinet, O. and Bounedine, K.S.: Classification of Brucella strains isolated from marine mammals by infrequent restriction site-PCR and development of specific PCR identification tests. Microb. Infect. 5, 593-602 (2003). https://doi.org/10.1016/S1286-4579(03)00091-1
  12. Whitehead, R.N., Overton, T.W., Kemp, C.L. and Webber, M.A.: Exposure of Salmonella enteric serovar Typhimurium to high level biocide challenge can select multidrug resistant mutants in a single step. PLoS ONE, 6, e22833 (2011). https://doi.org/10.1371/journal.pone.0022833
  13. Turkmani, A., Psaroulaki, A., Christidou, A., Samoilis, G., Mourad, T.A., Tabaa, D. and Tselentis, Y.: Uptake of ciprofloxacin and ofloxacin by 2 Brucella strains and their fluoroquinolone- resitant variants under different conditions. An in vitro study. Dign. Microbiol. Infect. Dis. 59, 447-451 (2007). https://doi.org/10.1016/j.diagmicrobio.2007.06.017
  14. Russell, A.D.: Biocide use and antibiotic resistance: the relevance of laboratory findings to clinical and environmental situations. Lancet Infect. Dis. 3, 794-803 (2003). https://doi.org/10.1016/S1473-3099(03)00833-8
  15. Giammanco, G., Pignato, S. and Giammanco, G.M.: Recent trends in salmonellosis epidemiology. J. Prev. Med. Hyg. 40, 19-24 (1999).
  16. Munozdel, R.M., Montano, M.F., Renteria, T.B., Sanchez, E., Moreno, J.F., Perez, A. and Saucedo, S.: Assessment of the economic impact of a brucellosis control program in a dairy herd using the partial budget method. J. Anim. Vet. Adv. 6, 146-151 (2007).
  17. Kahrs, R.F.: General disinfection guidelines. Rev. Sci. Tech. 14, 105-163 (1995). https://doi.org/10.20506/rst.14.1.836
  18. Ahmad, K.: Control of animal diseases caused by bacteria: Principles and approaches. Pak. Vet. J. 25, 200-202 (2005).
  19. Shams, A.M., O'Connellm H,, Arduino, M.J. and Rose, L.J.: Chlorine dioxide inactivation of bacterial threat agents. Lett. Appl. Microbiol. 53, 225-230 (2011). https://doi.org/10.1111/j.1472-765X.2011.03095.x
  20. Lindstedt, M., Allenmark, S., Thompson, R.A. and Edebo, L.: Antimicrobial activity of betaine esters, quaternary ammonium amphipheles which spontaneously hydrolyze into nontoxic components. Antimicrob. Agents Chemother. 34, 1949- 1954 (1990). https://doi.org/10.1128/AAC.34.10.1949
  21. Gotvajn, A.Z. and Zagorc-Kon an, J.: Laboratory simulation of biodegradation of chemicals in surface waters: closed bottle and respirometric test. Chemosphere, 38, 1339-1346 (1999). https://doi.org/10.1016/S0045-6535(98)00535-9
  22. Chang, A.S. and Schneider, K.R.: Evaluation of overhead spray-applied sanitizers for the reduction of Salmonella on tomato surfaces. J. Food Sci. 71, M45-69 (2012).
  23. Zaid, A.: Formulation and evaluation of the chemical stability of povidone-iodine in some trademarks cleaning formulations. Int. J. Pharm. Pharm. Sci. 5, 46-48 (2013).
  24. Cunliffe, P.J. and Fawcett, T.N.: Wound cleansing: the evidence for the techniques and solutions used. Prof. Nurse, 18, 95-99 (2002).
  25. Hah, J.H., Roh, D.H., Jung, Y.H., Kim, K.H. and Sung, M.W.: Selection of irrigation fluid to eradicate free cancer cells during head and neck cancer surgery. Head Neck, 34, 546-550 (2012). https://doi.org/10.1002/hed.21773
  26. Pattana-arun, J. and Wolff, B.G.: Benefits of povidone-iodine solution in colorectal operations: science or legend. Dis. Colon Rectum, 51, 966-971 (2008). https://doi.org/10.1007/s10350-008-9213-8
  27. Basha, G., Ghirardi, M., Geboes, K., Yap, S.H. and Penninckx, F.: Limitations of peritoneal lavage with antiseptics in prevention of recurrent colorectal cancer caused by tumorcell seeding: experimental study in rats. Dis. Colon Rectum, 43, 1713-1718 (2000). https://doi.org/10.1007/BF02236856
  28. Reimer, K., Wichelhaus, T.A., Schafer, V., Rudolph, P., Kramer, A., Wutzler, P., Ganzer, D. and Fleischer, W.: Antimicrobial effectiveness of povidone-iodine and consequences for new application areas. Dermatology, 204, 114-120 (2002). https://doi.org/10.1159/000057738
  29. Durani, P. and Leaper, D.: Povidone-iodine: use in hand disinfection, skin preparation and antiseptic irrigation. Int. Wound J. 5, 376-387 (2008). https://doi.org/10.1111/j.1742-481X.2007.00405.x
  30. Material Safety Data Sheet (MSDS): $Betadine^{(R)}$ solution (10% povidone iodine). Purdue Pharma L.P., Stamford, pp. 5-6 (2013).
  31. Xia, Y., Wei, L., Gao, X., Wu, H., Zheng, H. and Yu, Z.: Experimental study on toxicity of povidone iodine disinfectant. Chin. J. Disinfect. 22, 263-267 (2005).
  32. Park, K.H., Kim, S.R., Kang, S.Y., Jung, S.J. and Oh, M.J.: Toxicity of disinfectants in flounder (Paralichthys olivaceus), black rockfish (Sebastes pachycephalus) and black sea bream (Acanthopagrus schlegelii). J. Aquaculture, 21, 7-12 (2008).
  33. Heiner, J.D., Hile, D.C., Demons, S.T. and Wedmore, I.S.: 10% povidone-iodine may be a practical field water disinfectant. Wilderness Environ. Med. 21, 332-336 (2010). https://doi.org/10.1016/j.wem.2010.09.008
  34. Seimenis, A. and Skyrianos, G.: Effect of iodine (a polyvinylpyrrolidone- iodine complex) on Brucella strains. Bull. Back Abstract Descriptors Top Hell. Vet. Med. Soc. 31, 145-149 (1980).

Cited by

  1. Bactericidal Efficacy of a Disinfectant Spray Containing a Grapefruit-seed Extract, Citric acid, Malic acid and Benzalkonium Chloride against Salmonella Typhimurium and Brucella ovis vol.31, pp.4, 2016, https://doi.org/10.13103/JFHS.2016.31.4.299
  2. Bactericidal Efficacy of a Powder Disinfectant Containing Phosphate Compounds Against Salmonella Typhimurium vol.31, pp.6, 2016, https://doi.org/10.13103/JFHS.2016.31.6.471