치위생과학회지 (Journal of dental hygiene science)

  • 제14권2호
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  • Pages.223-229
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  • 2014
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  • 1598-4478(pISSN)
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  • 2233-7679(eISSN)
한국치위생과학회 (The Korean Society of Dental Hygiene Science)
권호 표지

Polycan-Calcium Gluconate 복합제에 의한 염증인자의 감소 효과

The Effect of Polycan-Calcium Gluconate Complex on Inflammatory Mediators from Periodontitis Patients

  • 김지혜 (경북대학교 치의학전문대학원 예방치과학교실) ;
  • 김기림 (경북대학교 치위생학과) ;
  • 진혜정 (동의대학교 치위생학과) ;
  • 임상욱 (경북대학교 치의학전문대학원 예방치과학교실) ;
  • 송근배 (경북대학교 치의학전문대학원 예방치과학교실) ;
  • 최연희 (경북대학교 치의학전문대학원 예방치과학교실)
  • Kim, Ji-Hye (Department of Preventive Dentistry, School of Dentistry, Kyungpook National University) ;
  • Kim, Ki-Rim (Department of Dental Hygiene, Kyungpook National University) ;
  • Jin, Hye-Jung (Department of Dental Hygiene, Dong-eui University) ;
  • Im, Sang-Uk (Department of Preventive Dentistry, School of Dentistry, Kyungpook National University) ;
  • Song, Keun-Bae (Department of Preventive Dentistry, School of Dentistry, Kyungpook National University) ;
  • Choi, Youn-Hee (Department of Preventive Dentistry, School of Dentistry, Kyungpook National University)
  • 투고 : 2014.05.13
  • 심사 : 2014.06.02
  • 발행 : 2014.06.30
⟨ 이전 논문 다음 논문 ⟩

초록

본 연구에서는 체내 염증지표 변화를 모니터링 함으로써 새로운 천연추출물인 polycan 및 calcium gluconate 복합제제의 치주질환 개선 효과를 평가하고자 하였으며, 다음과 같은 결론을 얻었다. 1. 치은열구액 내 $IL-1{\beta}$는 대조군과 시험군 모두 시간에 따른 유의한 차이는 없었으나(p>0.05), 4주째 시험군이 대조군보다 낮았다(p<0.05). 2. 치은열구액 내 MMP-8과 $TNF-{\alpha}$는 4주째 시험군에서 초기에 비해 감소하였으며, 시험군이 대조군보다 낮았다. 3. 혈액 내 MMP-8은 4주째 시험군에서 유의하게 감소하였고(p<0.05), 대조군에 비해 통계적으로 유의하게 낮았다(p<0.05). 그러나 혈액 내 CRP는 유의한 변화가 관찰되지 않았다(p>0.05). 이상의 결과들을 종합해 보았을 때, polycan을 함유한 calcium gluconate 복합제 복용이 전신적인 염증성 생체지표에 영향을 미쳐 치은열구액 내의 염증매개물질들을 감소시킴으로써 치은건강에 긍정적인 효과를 나타내는 것으로 사료된다.

The aim of this study was to examine the effect of polycan-calcium gluconate complex on the levels of immune and inflammatory mediators in gingival crevicular fluid and serum from patients with periodontitis. A total of 39 patients with periodontitis took placebo (placebo group) or polycan-calcium gluconate complex (treatment group) twice a day for 4 weeks. At baseline and 4 weeks, gingival crevicular fluid (GCF) and blood was collected from each subjects. The secretion level of interleukin $(IL)-1{\beta}$ in GCF was measured by enzyme-linked immunosorbent assay (ELISA). Western blot analysis was conducted to determine the expression of matrix metalloproteinase (MMP)-8 and tumor necrosis factor $(TNF)-{\alpha}$ in GCF. Serum samples were analysed for MMP-8 by ELISA and C-reactive protein (CRP) by turbidimetric immuno assay. MMP-8 and $TNF-{\alpha}$ were significantly decreased in the treatment group at 4 weeks. The level of $IL-1{\beta}$ in the treatment group was significantly lower than those of the placebo group. No differences were observed in CRP levels. Taken together, these data indicate that taking of polycan-calcium gluconate complex led to reduction of inflammatory biomarkers in serum and GCF of periodontitis patients.

키워드

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