Journal of Animal Environmental Science (한국축산시설환경학회지)

The Korean Society of Animal Environmental Science & Technology (한국축산환경학회)
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Isolation and Characterization of Phosphorus Accumulating Microorganisms under Liquid Fertilization of Swine Slurry

액비화 과정 중 인 이용 우수미생물 분리 및 특성

  • Lim, Joung-Soo (Animal Environment Division, National Institute of Animal Science, RDA) ;
  • Cho, Sung-Back (Animal Environment Division, National Institute of Animal Science, RDA) ;
  • Hwang, Ok-Hwa (Animal Environment Division, National Institute of Animal Science, RDA) ;
  • Yang, Seung-Hak (Animal Environment Division, National Institute of Animal Science, RDA)
  • 임정수 (농촌진흥청 국립축산과학원 축산환경과) ;
  • 조성백 (농촌진흥청 국립축산과학원 축산환경과) ;
  • 황옥화 (농촌진흥청 국립축산과학원 축산환경과) ;
  • 양승학 (농촌진흥청 국립축산과학원 축산환경과)
  • Received : 2014.05.10
  • Accepted : 2014.06.04
  • Published : 2014.06.30
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Abstract

This study was conducted to investigate the bacterial capability to accumulate phosphorus during liquid composting process of pig slurry. Storage liquid compost and pig slurry were analyzed by using MALDI-TOF technique, which showed the colonies of Acinetobacter towneri and Bacillus licheniformis. In addition, bacterial colonies were isolated under high phosphoric acid conditions using X-phosphate MOPS medium with the addition of 2 mM $K_2HPO_4$. Microbial growth was observed in high and low phosphoric conditions due to the growth of bacterial diversities in the liquid fertilizer and slurry. The colonies isolated in the high phosphoric acid medium were uncultured bacterium clone and Acinetobacter sp. were identified by analysis of 16S rRNA gene sequences. Uncultured bacterium showed higher growth rate and excellent phosphorus ability then Acinetobacter sp.. In addition to Paenibacillus sp. AEY-1 isolated from pig slurry performed excellent phosphorus utilizing capability.

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References

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