Korean journal of applied entomology (한국응용곤충학회지)

Korean Society of Applied Entomology (한국응용곤충학회)
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Halo Spot Symptom Induced by Oviposition of Frankliniella occidentalis on Grape Fruits: Molecular Diagnosis by a Species-specific DNA Amplification and Microscopic Characterization of the Symptom

꽃노랑총채벌레 산란에 의한 포도 과피 달무리 반점: 종 특이적 분자진단법을 이용한 종동정과 반점 증상의 형태적 특징

  • Ahn, Seung-Joon (Horticultural and Herbal Environment Division, National Institute of Horticultural and Herbal Science, RDA) ;
  • Cho, Myoung Rae (International Technology Cooperation Center, RDA) ;
  • Park, Cheol Hong (Horticultural and Herbal Environment Division, National Institute of Horticultural and Herbal Science, RDA) ;
  • Kang, Taek Jun (Horticultural and Herbal Environment Division, National Institute of Horticultural and Herbal Science, RDA) ;
  • Kim, Hyung Hwan (Horticultural and Herbal Environment Division, National Institute of Horticultural and Herbal Science, RDA) ;
  • Kim, Dong-Hwan (Horticultural and Herbal Environment Division, National Institute of Horticultural and Herbal Science, RDA) ;
  • Yang, Chang Yeol (Horticultural and Herbal Environment Division, National Institute of Horticultural and Herbal Science, RDA)
  • 안승준 (농촌진흥청 국립원예특작과학원 원예특작환경과) ;
  • 조명래 (농촌진흥청 기술협력국 국제기술협력과) ;
  • 박철홍 (농촌진흥청 국립원예특작과학원 원예특작환경과) ;
  • 강택준 (농촌진흥청 국립원예특작과학원 원예특작환경과) ;
  • 김형환 (농촌진흥청 국립원예특작과학원 원예특작환경과) ;
  • 김동환 (농촌진흥청 국립원예특작과학원 원예특작환경과) ;
  • 양창열 (농촌진흥청 국립원예특작과학원 원예특작환경과)
  • Received : 2014.05.23
  • Accepted : 2014.08.18
  • Published : 2014.09.01
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Abstract

In grape vineyards, whitish spots in a cloud shape have been often observed on the fruit surface recently. However, the cause of the halo spot symptom was unknown, hindering countermeasures to be properly designed for the control. A small hole in the middle of the formless halo spot remained as a scar formed by oviposition of the thrips. It became later a suberized scab, which is separated from the epidermal cells on the surface either to be retained on or to be detached from it as time proceeds. Such a symptom is distinguished from the feeding damages caused by thrips or true bugs occurring on the grape fruits. With DNA extracted from the egg-shell found in the hole, molecular diagnosis by amplifying an ITS2 region with universal primers and subsequently digesting the PCR product by an restriction enzyme (RsaI) revealed that the egg was laid by Frankliniella occidentalis. In addition, a mitochondrial COI sequence confirmed that the halo spot symptom was formed by its oviposition. This study provides accurate information on the peculiar damage symptom caused by oviposition of F. occidentalis that could be useful in the control strategies for this pest in vineyards.

최근 국내 포도원에서 과실 표면에 구름모양의 흰색 얼룩이 자주 발견되고 있으나, 그 원인에 대해 명확히 알려진 바가 없어 방제대책을 세우지 못하고 있는 실정이다. 흰색의 달무리 반점은 포도 과실 표면에 부정형으로 퍼져 있으며, 그 중앙에는 총채벌레가 산란할 때 만들어진 작은 구멍의 상처가 남아 있다. 이 상처부위는 시간이 지나면서 코르크화되고 표피세포와 분리되어 딱지로 남거나 떨어져 나가게 된다. 이러한 증상은 총채벌레의 섭식이나 노린재의 흡즙에 의한 상처와는 구별된다. 산란구멍에서 발견한 총채벌레 알껍질에서 DNA를 추출하여 ITS2 부위의 염기 서열을 PCR-RFLP 방법으로 분자동정을 실시한 결과 꽃노랑총채벌레의 것으로 확인되었다. 미토콘드리아 COI 염기서열은 이러한 분자 동정 결과를 재확인하여 주었다. 본 연구결과는 포도 과피에 꽃노랑총채벌레 산란에 의해 유발되는 독특한 피해 증상에 대한 정확한 정보를 제공하며 포도원에서 이 해충에 대한 방제전략 수립에 도움이 될 것으로 여겨진다.

Keywords

References

  1. Ahn, S.-J., Park, C.H., Kang, T.J., Kim, H.H., Yang, C.Y., Kim, D.-H., 2013. Occurrence of mites and thrips in protected grape vineyards in Korea. Proceedings of the Autumn Symposium of the Korean Society of Applied Entomology, p. 183.
  2. Bettiga, L.J., 2013. Grape pest management, 3rd ed., University of California, USA.
  3. Brunner, P.C., Fleming, C., Frey, J.E., 2002. A molecular identification key for economically important thrips species (Thysanoptera: Thripidae) using direct sequencing and a PCR-RFLP-based approach. Agric. For. Entomol. 4, 127-136. https://doi.org/10.1046/j.1461-9563.2002.00132.x
  4. Childers, C.C., 1997. Feeding and oviposition injuries to plants, in: Lewis, T. (Eds.), Thrips as crop pests. CABI International, Oxon, New York, pp. 505-537.
  5. Cho, M.R., Jeon, S.-W., Kang, T.J., Kim, H.H., Ahn, S.-J., Yang, C.Y., 2013. Pests occurring on Cymbidium. Korean J. Appl. Entomol. 52, 403-408. https://doi.org/10.5656/KSAE.2013.08.0.035
  6. Hilker, M., Meiners, T., 2002. Induction of plant responses to oviposition and feeding by herbivorous arthropods: a comparison. Entomol. Exp. Appl. 104, 181-192. https://doi.org/10.1046/j.1570-7458.2002.01005.x
  7. Hortensteiner, S., Krautler, B., 2011. Chlorophyll breakdown in higher plants. Biochim. Biophys. Acta-Bioenerg. 1807, 977-988. https://doi.org/10.1016/j.bbabio.2010.12.007
  8. Jensen, F., 1973. Timing of halo spotting by flower thrips on table grapes. Calif. Agr. 27, 6-8.
  9. Kim, D.S., Cho, M.R., Jeon, H.Y., Yiem, M.S., Lee, J.H., Na, S.Y., Lee, J.O., 2000. Damage patterns caused by Lygocoris spinolae (Hemiptera: Miridae) on 'Campbell Early' grapes. J. Asia-Pac. Entomol. 3, 95-101. https://doi.org/10.1016/S1226-8615(08)60062-X
  10. McNally, P.S., Fogg, C., Flynn, J., Horenstein, J., 1985. Effects of thrips (Thysanoptera: Thripidae) on shoot growth and berry maturity of 'Chenin Blanc' grapes. J. Econ. Entomol. 78, 69-72. https://doi.org/10.1093/jee/78.1.69
  11. Reitz, S.R., 2009. Biology and ecology of the western flower thrips (Thysanoptera: Thripidae): The making of a pest. Fla. Entomol. 92, 7-13. https://doi.org/10.1653/024.092.0102
  12. Salguero-Navas, V.E., Funderburk, J.E., Olson, S.M., Beshear, R.J., 1991. Damage to tomato fruit by the western flower thrips (Thysanoptera: Thripidae). J. Entomol. Sci. 26, 436-442. https://doi.org/10.18474/0749-8004-26.4.436
  13. Soler, M., Serra, O., Molinas, M., Huguet, G., Fluch, S., Figueras, M., 2007. A genomic approach to suberin biosynthesis and cork differentiation. Plant Physiol. 144, 419-431. https://doi.org/10.1104/pp.106.094227
  14. Terry, L.I., De Grandi-Hoffman, G., 1988. Monitoring western flower thrips (Thysanoptera: Thripidae) in 'Granny Smith' apple blossom clusters. Can. Entomol. 120, 1003-1016. https://doi.org/10.4039/Ent1201003-11
  15. Toda, S., Komazaki, S., 2002. Identification of thrips species (Thysanoptera: Thripidae) on Japanese fruit trees by polymerase chain reaction and restriction fragment length polymorphism of the ribosomal ITS2 region. B. Entomol. Res. 92, 359-363.
  16. Yang, C. Y., 2010. Occurrence of grape pests and their control, in: Ahn, J.G. (Eds), Manual of grape growing. Rural Development Adminstration. pp. 209-220.
  17. Yokoyama, V.Y., 1977. Frankliniella occidentalis and scars on table grapes. Environ. Entomol. 6, 25-30. https://doi.org/10.1093/ee/6.1.25
  18. Yokoyama, V.Y., 1979. Effect of thrips scars on table grape quality. J. Am. Soc. Hortic. Sci. 104, 243-245.

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