Korean journal of applied entomology (한국응용곤충학회지)

Korean Society of Applied Entomology (한국응용곤충학회)
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Effect of Cellular Phospholipase A2 Inhibition on Enhancement of Bt Insecticidal Activity

세포성 인지질분해효소 활성 억제에 따른 비티 살충력 증가 효과

  • Eom, Seonghyeon (Department of Bioresource Sciences, Andong National University) ;
  • Park, Jiyeong (Department of Bioresource Sciences, Andong National University) ;
  • Kim, Kunwoo (Department of Bioresource Sciences, Andong National University) ;
  • Kim, Yonggyun (Department of Bioresource Sciences, Andong National University)
  • 엄성현 (안동대학교 자연과학대학 생명자원과학과) ;
  • 박지영 (안동대학교 자연과학대학 생명자원과학과) ;
  • 김건우 (안동대학교 자연과학대학 생명자원과학과) ;
  • 김용균 (안동대학교 자연과학대학 생명자원과학과)
  • Received : 2014.05.12
  • Accepted : 2014.08.18
  • Published : 2014.09.01
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Abstract

Some bacterial metabolites of Xenorhabdus nematophila (Xn) inhibit phospholipase $A_2$ ($PLA_2$) activity to shutdown eicosanoid biosynthesis in target insects. However, little has been known about the target insect $PLA_2$ of these bacterial metabolites. Eight bacterial metabolites identified in Xn culture broth exhibited significant insecticidal activities against larvae of both lepidopteran species of Plutella xylostella and Spodoptera exigua. Moreover, these bacterial metabolites significantly enhanced insecticidal activities of Bacillus thuringiensis (Bt). To determine target $PLA_2$, we cloned and over-expressed cellular $PLA_2$ ($SecPLA_2$) of S. exigua. Purified $SecPLA_2$ catalyzed phospholipids derived from the fat body and released several polyunsaturated fatty acids. Most Xn metabolites significantly inhibited $SecPLA_2$ activity, but were different in their inhibitory activities. There was a positive correlation between the inhibition of $SecPLA_2$ and the enhancement of Bt insecticidal activity. These results indicate that $SecPLA_2$ is a molecular target inhibited by Xn metabolite.

곤충병원성세균 Xenorhabdus nematophila (Xn)의 일부 대사물질은 대상 곤충의 phospholipase $A_2$ ($PLA_2$)를 억제하여 아이코사노이드 생합성 활성을 저해시킨다. 그러나 이들 세균 대사물질이 억제하는 곤충의 $PLA_2$에 대해서는 알려져 있지 않다. Xn의 배양액에서 화학구조가 동정된 8 가지 대사물질들은 두 종의 나비목 배추좀나방(Plutella xylostella)과 파밤나방(Spodoptera exigua)의 유충에 대하여 살충 활성을 보유했다. 특별히 이들 물질은 모두 Bacillus thuringiensis (비티)의 살충력을 크게 향상시켰다. 파밤나방의 세포성 인지질 분해효소($SecPLA_2$)를 클로닝하고 대장균에서 과발현시켰다. 분리된 $SecPLA_2$를 지방체에서 얻은 인지질과 반응시켰을 때 여러 다가불포화지방산을 해리시켰다. 이 효소활성이 Xn 유래 대사물질들에 의해 뚜렷이 억제되었다. 또한 $SecPLA_2$에 대한 억제효과와 비티 살충력 상승효과 사이에 정의 상관관계를 보였다. 본 연구는 $SecPLA_2$가 Xn 대사물질의 억제 대상 분자 종말점 가운데 하나라고 제시하고 있다.

Keywords

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