Korean Journal of Fisheries and Aquatic Sciences (한국수산과학회지)

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
권호 표지
DOI QR코드

DOI QR Code

Cytogenetic Analysis of Starry Flounder Platichthys stellatus from Korea

강도다리(Platichthys stellatus)에 대한 세포유전학적 연구

  • Jung, Hyo Sun (Risk Assessment Center of Marine Living Modified Organisms, Pukyong National University) ;
  • Kim, Youn Kyoung (Risk Assessment Center of Marine Living Modified Organisms, Pukyong National University) ;
  • Kim, Hyun Chul (Genetics and Breeding Research Center, National Fisheries Research and Development Institute) ;
  • Noh, Jae-Koo (Genetics and Breeding Research Center, National Fisheries Research and Development Institute) ;
  • Lee, Jong-Ho (Genetics and Breeding Research Center, National Fisheries Research and Development Institute) ;
  • Kim, Dong Soo (Department of Marine Bio-Materials & Aquaculture, Pukyong National University)
  • 정효선 (부경대학교 해양용 LMO 위해성 평가 센터) ;
  • 김연경 (부경대학교 해양용 LMO 위해성 평가 센터) ;
  • 김현철 (국립수산과학원 육종연구센터) ;
  • 노재구 (국립수산과학원 육종연구센터) ;
  • 이정호 (국립수산과학원 육종연구센터) ;
  • 김동수 (부경대학교 해양바이오신소재학과)
  • Received : 2014.04.07
  • Accepted : 2014.05.27
  • Published : 2014.08.31
Download PDF
⟨ Previous Next ⟩

Abstract

Cytogenetic analysis was conducted to obtain basic information for chromosome manipulation of starry flounder Platichthys stellatus. Nuclear surface area and volume of erythrocyte were $7.60{\pm}0.93{\mu}m^2$ and $12.80{\pm}1.75{\mu}m^3$, respectively. The haploid DNA content of the species was 0.66 pg/haploid cell which correspond to 93% of olive flounder Paralichthys olivaceus. A karyotype analysis was also carried out with the species using conventional staining and Ag-NOR banding techniques. It was consisted of 48 acrocentric chromosomes and inter-sex or intra-individual polymorphism was not detected in all specimens analyzed. The NOR regions, appearing a terminal position of the short arm of the smallest acrocentric pairs.

Keywords

References

  1. Animal Genome Size Database, 2014. Vertebrates-Fishes haploid C-value. Retrieved from http://www.genomesize.com on March 27.
  2. Benfey TJ. 1989. A bibliography of triploid fish 1943 to 1988. Can Tech Rep Fish Aqua Sci 1682, 33.
  3. Byrappa V. 2003. Evolution and diversity of fish genomes. Curr Opin Genet Dev 13, 588-592. http://dx.doi.org/10.1016/j.gde.2003.09.001.
  4. Goodpasture C and Bloom SE. 1975. Visualization of nucleolar organizer regions in mammalian chromosomes using silver staining. Chromosoma 53, 37-50. https://doi.org/10.1007/BF00329389
  5. Kim DS, Park EH and Kim JS. 1982. Karyotypes of nine species of the Korean catfishes (Teleostomi:Siluriformes). Kor J Genet 4, 57-68.
  6. Kim DS, Kim JH, Jo JY, Moon YB and Cho KC. 1993. Induction of gynogenetic diploid in Paralichthys olivaceus. Gene & Genomics 48, 179-186.
  7. Kim PK. 2012. Growth performance and digestive characteristics of starry flounder Platichthys stellatus on the moist and extruded pellets. Kor J Fish Aquat Sci 45, 679-685. https://doi.org/10.5657/KFAS.2012.0679
  8. Lemoine HLJr and Smith LT. 1980. Polyploidy induced in brook trout by cold shock. Trans Fish Soc 109, 626-631. https://doi.org/10.1577/1548-8659(1980)109<626:PIIBTB>2.0.CO;2
  9. Li D. 2009. Studies on karyotype and digestive physiology of Platichthys stellatus Pallas. M.S. Thesis, Ocean University of China, Qingdao, China.
  10. Nam MM, Byun SG, Lee BI, Lee JH and Kim YC. 2008. Morphological characteristics of the hybrids of female flounder Paralichthys olivaceus and male starry flounder Platichthys stellatus. Kor J Ichthyol 20, 258-290.
  11. NFRDI. 2013. Olive flounder Paralichthys olivaceus Golden Seed project report. Golden Seed project report, 24-36, 1-533.
  12. Sezaki K and Kobayashi H. 1978. Comparison of erythrocytic size between diploid and tetraploid in spinous loach, Cobitis biwae. Bull Jap Soc Sci Fish 41, 851-854.
  13. Yamamoto E. 1999. Studies on sex-manipulation and production of cloned populations in hirame, Paralichthys olivaceus (Temminck et Schlegel). Aquaculture 173, 235-246. https://doi.org/10.1016/S0044-8486(98)00448-7