Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Novel Anticandidal Activity of a Recombinant Lampetra japonica RGD3 Protein

  • Wu, Caiping (Liaoning Provincial Key Labouratory of Biotechnology and Drug Discovery, Department of Biological Sciences, Liaoning Normal University) ;
  • Lu, Li (Department of Pharmacology, Dalian Medical University) ;
  • Zheng, Yuanyuan (Liaoning Provincial Key Labouratory of Biotechnology and Drug Discovery, Department of Biological Sciences, Liaoning Normal University) ;
  • Liu, Xin (Liaoning Provincial Key Labouratory of Biotechnology and Drug Discovery, Department of Biological Sciences, Liaoning Normal University) ;
  • Xiao, Rong (Liaoning Provincial Key Labouratory of Biotechnology and Drug Discovery, Department of Biological Sciences, Liaoning Normal University) ;
  • Wang, Jihong (Liaoning Provincial Key Labouratory of Biotechnology and Drug Discovery, Department of Biological Sciences, Liaoning Normal University) ;
  • Li, Qingwei (Liaoning Provincial Key Labouratory of Biotechnology and Drug Discovery, Department of Biological Sciences, Liaoning Normal University)
  • Received : 2013.12.13
  • Accepted : 2014.03.29
  • Published : 2014.07.28
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Abstract

Lj-RGD3, an RGD (Arg-Gly-Asp) toxin protein from the salivary gland of Lampetra japonica, exhibits antifungal activity against Candida albicans. Lj-RGD3 has three RGD motifs and shows homology to histidine-rich glycoprotein. We synthesised two mutant derivatives of Lj-RGD3: Lj-26, which lacks all three RGD motifs and contains no His residues; and Lj-112, which lacks only the three RGD motifs. We investigated the effects of the wild-type and mutated toxins on a gram-positive bacterium (Escherichia coli), a gram-negative bacterium (Staphylococcus aureus), and a fungus (C. albicans). rLj-RGD3 and its mutants exhibited antifungal but not antibacterial activity, as measured by a radial diffusion assay. The C. albicans inhibition zone induced by rLj-112 was larger than that induced by the other proteins, and its inhibitory effect on C. albicans was dose-dependent. In viable-count assays, the rLj-112 MIC was $7.7{\mu}M$, whereas the MIC of the positive control (ketoconazole) was $15{\mu}M$. Time-kill kinetics demonstrated that rLj-112 effectively killed C. albicans at $1{\times}$ and $2{\times}$ MIC within 12 and 6 h, respectively. Electron microscopy analysis showed that rLj-RGD3 and rLj-112 induced C. albicans lysis. Our results demonstrate a novel anticandidal activity for rLj-RGD3 and its mutant derivatives.

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References

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