Journal of the Korea Academia-Industrial cooperation Society (한국산학기술학회논문지)

The Korea Academia-Industrial cooperation Society (한국산학기술학회)
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Anti-corrugation activity of micosporine-like amino acid mixtures from Chlamydomonas sp.

Chlamydomonas sp. 유래 mycosporine-like amino acid 혼합물의 항주름 활성

  • Suh, Sung-Suk (South Sea Environment Research Department, Korea Institute of Ocean Science & Technology) ;
  • Seo, Hyo Hyun (Anti-aging Research Institute of BIO-FD&C Co.,Ltd.) ;
  • Lee, Jeong Hun (Anti-aging Research Institute of BIO-FD&C Co.,Ltd.) ;
  • Hwang, Jinik (South Sea Environment Research Department, Korea Institute of Ocean Science & Technology) ;
  • Park, Mirye (South Sea Environment Research Department, Korea Institute of Ocean Science & Technology) ;
  • Moh, Sang Hyun (Anti-aging Research Institute of BIO-FD&C Co.,Ltd.) ;
  • Lee, Taek-Kyun (South Sea Environment Research Department, Korea Institute of Ocean Science & Technology)
  • 서승석 (한국해양과학기술원 남해특성연구부) ;
  • 서효현 (바이오에프디엔씨 항노화연구소) ;
  • 이정훈 (바이오에프디엔씨 항노화연구소) ;
  • 황진익 (한국해양과학기술원 남해특성연구부) ;
  • 박미례 (한국해양과학기술원 남해특성연구부) ;
  • 모상현 (바이오에프디엔씨 항노화연구소) ;
  • 이택견 (한국해양과학기술원 남해특성연구부)
  • Received : 2014.07.14
  • Accepted : 2014.08.07
  • Published : 2014.08.31
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Abstract

To examine the effects of a mycosporine-like amino acids (MAAs) mixture from microalgae, Chlamydomonas sp, on the anti-wrinkle activities, the expression levels of genes that are associated with skin aging, including type I procollagen, elastin and involucrin, were analyzed. Asterina 330+palythine (A+P) and shinorine+palythine (S+P) mixtures were purified from Chlamydomonas sp using the following steps: 80% methanolic extraction, column purification, and HPLC analysis. As a result of the MTT assay, A+P and S+P did not induce cellular cytotoxicity with up to 0.1 mg/mL of both MAAs. In addition, the treatment of UV-exposed fibroblasts with A+P (0.05 mg/mL) and S+P (0.01 mg/mL) increased the levels of the PCOLCE mRNAs by 2.7 and 3.6 fold compared to the control group, respectively, The levels of elastin gene expression were elevated 5.59 and 3.1 fold in the A+P and S+P treated (0.01 mg/mL) cells, respectively. In particular, at a concentration of 0.01 mg /mL, the A+P and S+P expression levels of Involucrin mRNAs were increased 3.5 and 2.5 fold in the UV-exposed cells compared to the control, respectively. In conclusion, the MAAs derived from Chlamydomonas sp can be utilized as functional cosmetic materials with anti-wrinkle effects on the skin.

미세조류 유래 mycosporine-like amino acids (MAAs) 혼합물의 항주름활성을 분석하기 위하여 type I procollagen, elastin 및 involucrin 유전자 발현분석을 실시하였다. Chlamydomonas sp.를 80% 에탄올로 추출하고, 컬럼을 통해 분획물을 정제하고, HPLC 분석을 통해 asterina 330+palythine (A+P) 및 shinorine+palythine (S+P) 혼합물을 정제하였다. MTT assay 결과 A+P 및 S+P는 0.1 mg/mL까지 세포독성을 나타내지 않았다. UV에 노출된 섬유아세포에 대한 MAAs 혼합물의 영향을 분석하였을 때, 0.05 mg/mL A+P 및 0.01 mg/mL S+P 농도에서 각각 2.7 및 3.6 배 PCOLCE mRNAs 발현이 증가하였다. Elastin 유전자는 0.01 mg/mL의 A+P 및 S+P를 처리하였을 때 각각 5.59배 및 3.1배 발현이 증가하였다. 특히 0.01 mg/mL의 A+P 및 S+P 농도에서 involucrin mRNAs 발현이 UV 처리된 대조구와 비교하였을 때 5배 및 2.5배 감소하였다. 결론적으로 Chlamydomonas sp. 유래 MAAs 혼합물은 주름개선용 기능성 화장품 개발을 위한 소재로써의 가능성이 충분한 것으로 판단되었다.

Keywords

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