The Korean Journal of Food And Nutrition (한국식품영양학회지)

The Korean Society of Food and Nutrition (한국식품영양학회)
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Comparison of Antioxidant and Antimicrobial Activities in Siraegi (Dried Radish Greens) according to Cooking Process

조리 과정 중 시래기의 항산화 활성 및 항균 활성 비교

  • Park, Cho-Hee (Dept. of Food and Nutrition, Chungnam National University) ;
  • Kim, Kyoung-Hee (Dept. of Food and Nutrition, Chungnam National University) ;
  • Yook, Hong-Sun (Dept. of Food and Nutrition, Chungnam National University)
  • 박초희 (충남대학교 식품영양학과) ;
  • 김경희 (충남대학교 식품영양학과) ;
  • 육홍선 (충남대학교 식품영양학과)
  • Received : 2013.11.22
  • Accepted : 2014.08.08
  • Published : 2014.08.30
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Abstract

We evaluated the yield of extract, antioxidant compounds (total phenolic, total flavonoid), antioxidant (DPPH assay, ABTS assay, Oyaizu's assay, FRAP assay), and antimicrobial activities of Siraegi (dried radish greens) according to cooking process (non-blanched, blanched, seasoned). The yield of non-blanched Siraegi was 4.91%, blanched Siraegi was 0.33%, and seasoned Siraegi was 7.55%. In total polyphenol and flavonoid contents, seasoned Siraegi extracts showed higher antioxidant compounds ($129.85{\pm}0.62mg$ GAE/100 g FW, $35.56{\pm}1.19mg$ CHE/100 g FW) than non-blanched and blanched. Total antioxidant activities (DPPH assay, ABTS assay, FRAP assay, reducing power) were shown to be in the order of seasoned Siraegi > non-blanched Siraegi > blanched Siraegi. In antimicrobial activity, non-blanched Siraegi (5, 10 mg/disc) showed antimicrobial activity against B. cereus, E. cloacae, and E. coli (9.25 mm), and P. aeruginosa (9 mm) at 10 mg/disc. In terms of antimicrobial activity, non-blanched Siraegi was good but eating the dried vegetable was difficult so it is essential to blanch it. Also, with many added seasonings in the process of cooking, it can be easy to eat. Overall, the results of this study demonstrate that cooked Siraegi with seasoning would be the most efficient way of ingesting the antioxidant material.

본 실험에서는 조리과정(non-blanched, blanched, seasoned) 중 시래기의 항산화 관련 물질 함량과 항산화 활성 및 항균활성의 변화를 측정하여 한식 고유의 조리법인 나물의 우수성을 알리고자 하였다. 80% ethanol에 추출한 시래기의 수율은 무친 후가 7.55%로 가장 높았으며, 데치기 전이 4.91%를 나타내었고, 데친 후가 0.33%로 가장 낮은 수율을 보였다. 총 폴리페놀 함량은 3.57~129.85 mg GAE/100 g FW 범위로 무친 후 시래기가 $129.85{\pm}0.62mg$ GAE/100 g FW로 가장 높게 나타났으며, 총 플라보노이드 함량에서도 무친 후가 $35.56{\pm}1.19mg$ CHE/100 g FW로 가장 높은 함량을 나타냈다. 4가지의 항산화 실험(DPPH assay, ABTS assay, FRAP assay, Reducing power) 결과, 무친 후 > 데치기 전 > 데친 후 순으로 높은 활성을 보였다. 항균 활성 측정 결과, 데치기 전 시래기에서만 활성이 나타났는데, B. cereus와 E. cloacae 두 균에 대해서 농도 의존적으로 항균 활성을 나타냈고, E. coli와 P. aeruginosa 두 균에 대해서는 10 mg/disc의 농도에서만 각각 9.25 mm, 9 mm의 clear zone을 생성하였다. 상관관계 분석 결과, 총 폴리페놀과 총 플라보노이드 함량이 증가할수록 radical 소거 활성 및 환원력의 활성이 증가하여 시래기에 함유된 총 폴리페놀 및 플라보노이드 함량이 항산화 활성에 중요한 영향을 미치는 것으로 사료된다. 따라서 항균 활성 측면에서는 시래기를 조리하지 않고 섭취하는 것이 좋으나, 건조 나물을 섭취하기 위해서는 특성상 반드시 불린 후 조리하는 작업이 필요하다. 또한 무치거나 조리하는 과정에서 갖은 양념이 첨가되어 항산화 효능이 증가하므로 시래기를 섭취할 경우, 가능하면 장시간 물에 삶는 조리법은 피하고, 무치는 조리법을 사용하는 것이 건강에 이로울 것으로 사료된다.

Keywords

References

  1. Arano MB, Cano A, Acosta M. 2001. The hydrophilic and liphophilic contribution to total antioxidant activity. Food Chem 73:239-244 https://doi.org/10.1016/S0308-8146(00)00324-1
  2. Benzie IFF, Strain JJ. 1996. The ferric reducing ability of plasma (FRAP) as a measure of "antioxidant power": the FRAP assay. Anal Biochem 230:70-79
  3. Blois MS. 1958. Antioxidant determinations by the use of a stable free radical. Nature 181:1199-1200 https://doi.org/10.1038/1811199a0
  4. Bogin E, Abrams M. 1976. The effect of garlic extract on the activity of some enzymes. Food Cosmet Toxicol 14:417-419 https://doi.org/10.1016/S0015-6264(76)80178-2
  5. Cha YJ, Lee HY, Jeong EJ. 2008. Changes of biological activites of two ascidians, Styela clava and Styela plicata, by cooking. J Life Sci 18:109-113 https://doi.org/10.5352/JLS.2008.18.1.109
  6. Choi NS, Oh SS, Lee JM. 2001. Changes of biologically functional compounds and quality properties of aster scaber (Chamchwi) by blanching conditions. Korean J Food Sci Technol 33:745-752
  7. Chung YC, Chiang BH, Wei JH, Wang CK, Chen PC, Hsu CK. 2008. Effect of blanching, drying and extraction processes on the antioxidant activity of yam (Dioscorea alata). Int J Food Sci Technol 43:859-864 https://doi.org/10.1111/j.1365-2621.2007.01528.x
  8. Fellegrini N, Roberta R, Min Y, Catherine RE. 1998. Screening of dietary carotenoids and carotenoid-rich fruit extract for antioxidant activities applying 2,2'-azinobis (3-ethylenbenzo thiazoline-6-sulfonic acid) radical cation decolorization assay. Method Enzymol 299:379-389
  9. Folin O, Denis W. 1912. On phosphotungstic-phospho phosphomolybdic compounds as color reagents. J Biol Chem 12: 239-243
  10. Han YL, Lee SY, Lee JH, Lee SJ. 2013. Cellular flavonoid transport mechanisms in animal and plant cells. Korean J Food Sci Technol 45:137-141 https://doi.org/10.9721/KJFST.2013.45.2.137
  11. Hong JJ, Ahn TH. 2005. Changes in total flavonoid and total polyphenol contents of leafy vegetables (spinach, chard and whorled mallow) by blanching time. Korean J Food Cookery Sci 21:190-194
  12. Hong SH, Kim MJ, Oh CH, Yoon SH, Song YO. 2010. Antiradical capacities of perilla, sesame and sun flower oil. J Food Sci Nutr 15:51-56 https://doi.org/10.3746/jfn.2010.15.1.051
  13. Hoult JBS, Paya M. 1996. Pharmacological and biochemical actions of simple coumarins: Natural products with therapeutic potential. Gen Pharmacol 27:719-722
  14. Kang DY, Shin MO, Shon JH, Bae SJ. 2009. The antioxidative and antimicrobial effects of celastrus orbiculatus. J Life Sci 19:52-57 https://doi.org/10.5352/JLS.2009.19.1.052
  15. Kang MJ, Yun HS, Shin JH. 2012. Chemical properties and biological activity of garlic (Allium sativum L.) shoots. J Agric Life Sci 46:129-139
  16. Kim HJ. 2010. Study of anti-oxidant and anti-microbial activity of sulfur containing vegetables. Ms Thesis, Chungnam Univ. Daejeon, Korea
  17. Kim JH, Kim JK, Kang WW, Ha YS, Choi SW, Moon KD. 2003. Chemical compositions and DPPH radical scavenger activity in different sections of safflower. J Korean Soc Food Sci Nutr 32:733-738 https://doi.org/10.3746/jkfn.2003.32.5.733
  18. Kim JS. 2012. Greens Notebook (Namul Suchup). pp.204-205. Woodumji
  19. Kim JY, Park SH, Lee KT. 2009. Sulforaphane content and antioxidative effect of cooked broccoli. J East Asian Soc Dietary Life 19:564-569
  20. Ku KH, Lee KA, Kim YE. 2008. Physiological activity of extracts from radish (Raphanus sativus L.) leaves. J Korean Soc Food Sci Nutr 37:390-395 https://doi.org/10.3746/jkfn.2008.37.3.390
  21. Ku KH, Lee KA, Kim YL, Lee YW. 2006. Quality characteristics of hot-air dried radish (Raphanus sativus L.) leaves. J Korean Soc Food Sci Nutr 34:780-785 https://doi.org/10.3746/jkfn.2006.35.6.780
  22. Lee BH, Kim DR, Kang SM, Kim MR, Hong JI. 2010. Changes in the chemical stability and antioxidant activities of curcuminoids under various processing conditions. Korean J Food Sci Technol 42:97-102
  23. Lee YM, Bae JH, Jung HY, Kim JH, Park DS. 2011. Antioxidant activity in water and methanol extracts from Korean edible wild plants. J Korean Soc Food Sci Nutr 40:29-36 https://doi.org/10.3746/jkfn.2011.40.1.029
  24. Lee YM, Bae JH, Kim JB, Kim SY, Chung MN, Park MY, Ko JS, Song J, Kim JH. 2012. Changes in the physiological activities of four sweet potato varieties by cooking condition. Korean J Nutr 45:12-19 https://doi.org/10.4163/kjn.2012.45.1.12
  25. Oboh G. 2004. Effect of blanching on the antioxidant properties of some tropical green leafy vegetables. Science Direct 38:513-517
  26. Oh HJ, Kim CS. 2007. Antioxidant and nitrite scavenging ability of fermented soybean foods (Chungkukjang, Doenjang). J Korean Soc Food Sci Nutr 36:1503-1510 https://doi.org/10.3746/jkfn.2007.36.12.1503
  27. Oyaizu M. 1986. Studies on product of browning reaction prepared from glucose amine. Jap J Nutr 44:307-315 https://doi.org/10.5264/eiyogakuzashi.44.307
  28. Park JW, Lee YJ, Yoon S. 2007. Total flavonoids and phenolics in fermented soy products and their effects on antioxidant activities determined by different assays. Korean J Food Culture 22:353-358
  29. Park SW, Yoo YJ. 1997. Effect of pre-treatment methods before cooking on mineral retention in Siraegi (raddish leaves). Korean J Soc Food Sci 13:635-638
  30. Sakiko A, Yukikp Y. 2007. Antioxidant activity and flavonoid content of welsh onion (Allium fistulosum) and the effect of thermal treatment. Food Sci Technol Res 13:67-72 https://doi.org/10.3136/fstr.13.67
  31. Sher A. 2004. Antimicrobial activity of natural products from medicinal plants. Gomal J Med Sci 7:72-78
  32. Yoon HS. 2012. Greens Cooking (Namul Yori). pp.128-129. Haseo
  33. Zhishen J, Mengcheng T, Jianming W. 1999. The determination of flavonoid contents in mulberry and they scavenging effects on superoxide radicals. Food Chem 64:555-559 https://doi.org/10.1016/S0308-8146(98)00102-2

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