한국발생생물학회지:발생과생식 (Development and Reproduction)

  • 제18권2호
  • /
  • Pages.117-125
  • /
  • 2014
  • /
  • 2465-9525(pISSN)
  • /
  • 2465-9541(eISSN)
한국발생생물학회 (The Korean Society of Developmental Biology)
권호 표지
DOI QR코드

DOI QR Code

Improvement of the Vitrification Method Suppressing the Disturbance of Meiotic Spindle and Chromosome Systems in Mature Oocytes

  • Jung, Yun Jin (Division of Developmental Biology and Physiology, School of Biosciences and Chemistry, Institute of Basic Science, College of Natural Sciences, Sungshin Women's University) ;
  • Cheon, Yong-Pil (Division of Developmental Biology and Physiology, School of Biosciences and Chemistry, Institute of Basic Science, College of Natural Sciences, Sungshin Women's University)
  • 투고 : 2014.05.15
  • 심사 : 2014.05.30
  • 발행 : 2014.06.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Vitrification method is widely used in oocyte cryopreservation for IVF but the birth rates are lower than that of the fresh oocyte. One of the known main reasons is structural instability of meiotic spindle and chromosome systems of mature oocyte. To get the best way for keeping competence of matured oocytes, we studied the best conditions for vitrification focused on equilibration times. The mature oocytes were underwent vitrification with current popular method and analyzed the survival rates, microtubule stability and DNA integrity. The survival rates of recovered oocyte are almost same between groups and are more than 93%. The structural configuration of meiotic spindle was well kept in 10 min equilibration group and the stability rate was almost same with that of control. The chromosomal breakdown was observed in all experimental groups, but the chromosomal stability was higher in 10 min equilibration group than the other groups. The 10 min equilibration group showed best condition compared with the other groups. Based on these results, the equilibration time is one of the key factors in successful keeping for competence of mature oocyte. Although, more fine analysis about the effects of physical stress on oocyte during vitrification is needed to define the optimal condition, it is suggested that the optimal equilibration time to get competent oocyte in mouse is 10 min. Information acquired this study may provide insight into intracellular structural events occurring in human oocytes after vitrification and application for cryopreservation of human oocyte.

키워드

참고문헌

  1. Aman R, Parks J (1994) Effects of cooling and rewarming on the meiotic spindle and chromosomes of in vitro matured bovine oocytes. Biol Reprod 50:103-110. https://doi.org/10.1095/biolreprod50.1.103
  2. Al-Hasani S, Diedrich K, Van der Ven H, Krebs D (1986) Initial results of the cryopreservation of human oocytes. Geburtshilfe Frauenheikd 46:643-644. https://doi.org/10.1055/s-2008-1036272
  3. Bernard A, Fuller B (1996) Cryopreservation of human oocytes: a review of current problems and perspectives. Hum Reprod Update 2:193-207. https://doi.org/10.1093/humupd/2.3.193
  4. Bianchi V, Coticchio G, Fava L, Flamigni C, Borini A (2005) Meiotic spindle imaging in human oocytes frozen with a slow freezing procedure involving high sucrose concentration. Hum Reprod 220:1078-1083.
  5. Boldt J, Cline D, McLaughlin D (2003) Human oocyte cryopreservation as an adjunct to IVF-embryo transfer cycles. Hum Reprod 18:1250-1255. https://doi.org/10.1093/humrep/deg242
  6. Boldt J, Tidswell N, Sayears A, Kilani R, Cline D (2006) Human oocyte cryopreservation: 5-year experience with a sodium-depleted slow freezing method. Reprod Biomed online 13:96-100. https://doi.org/10.1016/S1472-6483(10)62021-4
  7. Borini A, Bonu MA, Coticchio G, Bianchi V, Cattoli M, Flamigni C (2004) Pregnancies and births after oocyte cryopreservation. Fertil Steril 82:601-605. https://doi.org/10.1016/j.fertnstert.2004.04.025
  8. Borini A, Sciajno R, Bianchi V, Sereni E, Flamigni C, Coticchio G (2006) Clinical outcome of oocyte cryo preservation after slow cooling winchith a protocol utilizing a high sucrose concentration. Hum Reprod 21:512-517. https://doi.org/10.1093/humrep/dei346
  9. Chen C (1986) Pregnancy after human oocyte cryopreservation. Lancet 1:884-886.
  10. Diedrich K, Al-Hasani S, Van der Ven D (1988) Successful in vitro fertilization of frozen-thawed rabbit and human oocytes. Ann NY Acad Sci 541:562-570. https://doi.org/10.1111/j.1749-6632.1988.tb22292.x
  11. Eroglu A, Toth TL, Toner M (1998) Alterations of the cytoskeleton and polyploidy induced by cryopreservation of metaphase II moue oocytes. Fertil Steril 69:944-957. https://doi.org/10.1016/S0015-0282(98)00030-2
  12. Fabbri R, Porcu E, Marsella T, Rocchetta G (2001) Human oocyte cryopreservation: new perspectives regarding oocyte survival. Hum Reprod 16:411-416. https://doi.org/10.1093/humrep/16.3.411
  13. Fosas N, Marina F, Torres PJ, Jove I, Martin P, Perez N, Arnedo N, Marina S (2003) The births of five spanish babies from cryopreserved donated oocytes. Hum Reprod 18:1417-1421. https://doi.org/10.1093/humrep/deg297
  14. Huang YJ, Chen HY, Park JY, Tan SL, Chian RC (2008) Comparison of spindle and chromosome configuration in in vitro- and in vivo-matured mouse oocytes after vitrification. Fertil Stertil 90:1424-1432. https://doi.org/10.1016/j.fertnstert.2007.07.1335
  15. Katayama KP, Stehlik J, Kuwayama M, Kato O, Stehlik E (2003) High survival rate of vitrified human oocytes results in clinical pregnancy. Fertil Steril 80:223-224.
  16. Kuleshova LL, Lopata A (2002) Vitrification can be more favorable than slow cooling. Fertil Steril 78:44-54.
  17. Kuwayama M, Vajta G, Ieda S, Kato O (2005) Comparison of open and closed methods for vitrification of human embryos and the elimination of potential contamination. Reprod Biomed Online 11:608-614. https://doi.org/10.1016/S1472-6483(10)61169-8
  18. Lucena E, Bernal DP, Lucena C, Rojas A, Moran A, lUCENA A (2006) Successful ongoing pregnancies after vitrification of oocytes. Fertil Stertil 85:108-111. https://doi.org/10.1016/j.fertnstert.2005.09.013
  19. Magistrini M, Szollosi D (1980) Effects of cold and of isopropyl-N-phenylacarbamate on the second meiotic spindle of mouse oocytes. Eur J Cell Biol 22:699-707.
  20. Mazur P, Leibo S, Chu E (1972) A two-factor hypothesis of freezing injury: evidence from Chineses hamster tissue-culture cells. Exp Cell Res 71:345-355. https://doi.org/10.1016/0014-4827(72)90303-5
  21. Mandelbaum J, Belaisch-Allart J, Junca AM, Antoine JM, Plachot M, Alvarez S, Alnot MO, Salat-Baroux J (1998) Cryopreservation in human assisted reproduction is now routine for embryos but remains a research procedure for oocytes. Hum Reprod 13:161-174.
  22. Meryman HT (1971) Cryoprotective agents. Cryobiology 8:173-83. https://doi.org/10.1016/0011-2240(71)90024-1
  23. Oktay K, Cil AP, Bang H (2006) Efficiency of oocyte cryopreservation a meta-analysis. Fertil Steril 86:70-80. https://doi.org/10.1016/j.fertnstert.2006.03.017
  24. Pickering SJ, Braude PR, Johnson MH, Cant A, Currie J (1990) Transient cooling to room temperature can cause irreversible disruption of the meiotic spindle in the human oocyte. Fertil Stertil 54:102-108.
  25. Pickering S, Johnson M (1987) The influence of cooling on the organization of the meiotic spindle of the mouse oocytes. Hum Reprod 2:207-216.
  26. Porcu E, Fabbri R, Seracchioli R, Ciotti PM, Magrini O, Flamigni C (1997) Birth of a healthy female after intracytoplasmic sperm injection of cryopreserved human oocytes. Fertil Steril 68:724-726. https://doi.org/10.1016/S0015-0282(97)00268-9
  27. Sathananthan AH, Ng SC, Trounson AO, Bongso A, Ratnam SS, Ho J, Mok H, Lee MN (1988) The effects of ultrarapid freezing on meiotic and mitotic spindles of mouse oocytes and embryos. Gamete Res 21:385-401. https://doi.org/10.1002/mrd.1120210407
  28. Singh NP, McCoy MT, Tice RR, Schneider EL (1988) A simple technique for quantitation of low levels of DNA damage in individual cells. Exp Cell Res 175:184-191. https://doi.org/10.1016/0014-4827(88)90265-0
  29. Stachecki JJ, Cohen J, Garrisi J, Munne S, Burgess C, Willadsen SM (2006) Cryopreservation of unfertilized human oocytes. Reprod Biomed Online 13:222-227. https://doi.org/10.1016/S1472-6483(10)60619-0
  30. Veeck LL (2003) Does the developmental stage at freeze impact on clinicla results post-thaw? Reprod Biomen Online 6:367-374. https://doi.org/10.1016/S1472-6483(10)61859-7
  31. Vincent C, Garnier V, Heyman Y, Renard J (1989) Solvent effects on cytoskeletal organization and in-vivo survival after freezing of rabbit oocytes. J Reprod Fertil 87:809-820. https://doi.org/10.1530/jrf.0.0870809
  32. Vincent C, Johnson MH (1992) Cooling, cryoprotectants, and the cytoskeleton of the mammalian oocyte. Oxford Rev Reprod Biol 14:73-100.
  33. Webb M, Howlett SK, Maro B (1986) Parthenogenesis and cytoskeletal organization in agein mouse eggs. J Embryol Exp Morphol 95:131-145.
  34. Wennerholm WB (2000) Cryopreservation of embryos and oocytes:obstetric outcome and health in children. Hum Reprod 15:18-25.
  35. Yoon TK, Kim TJ, Park SE, Hong SW, Ko JJ, Chung HM, Cha KY (2003) Live births after vitrification of oocytes in a stimulated in vitro fertilization-embryo transfer program. Fertil Steril 79: 1323-1326. https://doi.org/10.1016/S0015-0282(03)00258-9
  36. Zenses MT, Bielecki R, Casper R, Leibo SP (2001) Effects of chilling to 0?C on the morphology of meiotic spindles in human metaphase II oocytes. Fertil Steril 75:769-777. https://doi.org/10.1016/S0015-0282(00)01800-8