한국발생생물학회지:발생과생식 (Development and Reproduction)

  • 제18권2호
  • /
  • Pages.89-98
  • /
  • 2014
  • /
  • 2465-9525(pISSN)
  • /
  • 2465-9541(eISSN)
한국발생생물학회 (The Korean Society of Developmental Biology)
권호 표지
DOI QR코드

DOI QR Code

Genetic Distances of Three White Clam (Meretrix lusoria) Populations Investigated by PCR Analysis

  • Kim, Dae-Hyun (Department of Aquatic Life Medicine, College of Ocean Science and Technology, Kunsan National University) ;
  • Yoon, Jong-Man (Department of Aquatic Life Medicine, College of Ocean Science and Technology, Kunsan National University)
  • 투고 : 2014.04.03
  • 심사 : 2014.04.30
  • 발행 : 2014.06.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

The twenty-one individuals of Meretrix lusoria were secured from Gunsan, Shinan and Yeonggwang on the coast of the Yellow Sea and the southern sea in the Korean Peninsula, respectively. Amplification of a single COI fragment (720 bp) was imagined, and no apparent size differences were observed in amplified fragments between Meretrix lusoria and M. petechialis individuals. The size of the DNA fragments also varied excitedly, from 200 to 1,600 bp. The oligonucleotides primer BION-08 produced the least loci (a total of 17), with an average of 2.43 in the Gunsan population, in comparison to the other primers used. Remarkably, the primer BION-13 detected 42 shared loci by the three populations, major and/or minor fragments of sizes 200 bp and 400 bp, respectively, which were identical in all samples. The dendrogram gained by the seven oligonucleotides primers highlight three genetic clusters: cluster 1 (GUNSAN 01 ~ GUNSAN 07), cluster 2 (SHINAN 08 ~ SHINAN 14) and cluster 3 (YEONGGWANG 15 ~ YEONGGWANG 21). The longest genetic distance among the twenty-one Meretrix lusoria individuals that displayed significant molecular differences was between individuals GUNSAN no. 01 and SHINAN no. 14 (genetic distance = 0.574). Comparatively, individuals of SHINAN population were fairly closely related to that of YEONGGWANG population. In this study, PCR analysis has discovered significant genetic distances between two white clam population pairs (P<0.05).

키워드

참고문헌

  1. Callejas C, Ochando MD (1998) Identification of Spanish barbel species using the RAPD technique. J Fish Biol 53:208-215. https://doi.org/10.1111/j.1095-8649.1998.tb00121.x
  2. Chen AH, Li ZX, Feng GN (2009) Phylogenetic relationships of the genus Meretrix (Mollusca: Veneridae) based on mitochondrial COI gene sequences. Zool Res 30:233-239. https://doi.org/10.3724/SP.J.1141.2009.03233
  3. Chenyambuga SW, Hanotte O, Hirbo J, Watts PC, Kemp SJ, Kifaro GC, Gwakisa PS, Petersen PH, Rege JEO (2004) Genetic characterization of indigenous goats of sub-Saharan Africa using microsatellite DNA markers. Asian-Aust J Anim Sci 17:445-452. https://doi.org/10.5713/ajas.2004.445
  4. Folmer O, Black M, Hoeh W, Lutz R, Vrijenhoek R (1994) DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Mole Mar Biol Biotechnol 3:294-299.
  5. Huang BX, Peakall R, Hanna PJ (2000) Analysis of genetic structure of blacklip abalone (Haliotis rubra) populations using RAPD, minisatellite and microsatellite markers. Mar Biol 136:207-216. https://doi.org/10.1007/s002270050678
  6. Jung HT, Kim J, Choi SD (2004) Phylogenetic relationship of the five Korean Veneridae clams, Bivalvia, Veneroida according to morphological characters. J Aquacul 17: 197-208.
  7. Kang SK, Yoon JM (2013) Geographic variations of three Fulvia mutica populations. Korean J Malacol 29(3): 163-169. https://doi.org/10.9710/kjm.2013.29.3.163
  8. Kim JY, Park CY, Yoon JM (2004) Genetic differences and DNA polymorphism in oyster (Crassostrea spp.) analysed by RAPD-PCR. Korean J Genet 26:123-134.
  9. McCormack GC, Powell R, Keegan B (2000) Comparative analysis of two populations of the brittle star Amphiura filiformis (Echinodermata: Ophiuroidae) with different life history strategies using RAPD markers. Mar Biotechnol 2:100-106.
  10. Min DK, Lee JS, Koh DB, Je JK (2004) Mollusks in Korea. Min Molluscan Research Institutte.
  11. Jeffreys AJ, Morton DB (1987) DNA fingerprints of dogs and cats. Anim Genet 18:1-15.
  12. Muchmore ME, Moy GW, Swanson WJ, Vacquier VD. (1998) Direct sequencing of genomic DNA for characterization of a satellite DNA in five species of Eastern Pacific abalone. Mol Mar Biol Biotechnol 7(1):1-6.
  13. Park GS, Yoon JM (2008) Geographic variations between jedo venus clam (Protothaca jedoensis Lischke) populations of Boryeong and Wonsan of Korea. Korean J Malacol 24:13-26.
  14. Wieckowski MR, Giorgi C, Lebiedzinska M, Duszynski J, Pinton P (2009) Isolation of mitochondria-associated membranes and mitochondria from animal tissues and cells. Nat Prot 4(11):1582-1590. https://doi.org/10.1038/nprot.2009.151
  15. Yamakawa AY, Imai H (2012) Hybridization between Meretrix lusoria and the alien congeneric species M. petechialis in Japan as demonstrated using DNA markers. Aquat Inv 7 Iss 3:327-336.
  16. Yoke-Kqueen C, Radu S (2006) Random amplified polymorphic DNA analysis of genetically modified organisms. J Biotechnol 127:161-166. https://doi.org/10.1016/j.jbiotec.2006.06.001
  17. Yoon JM, Kim JY (2004) Genetic differences within and between populations of Korean catfish (S. asotus) and bullhead (P. fulvidraco) analysed by RAPD-PCR. Asian- Aust J Anim Sci 17:1053-1061. https://doi.org/10.5713/ajas.2004.1053