Korean Journal of Ichthyology (한국어류학회지)

The Ichthyological Society of Korea (한국어류학회)
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Identification of Hybrid between the Tiger Grouper Epinephelus fuscoguttatus and the Giant Grouper E. lanceolatus by Analyzing COX I and RAG 2 Sequences

COX I 및 RAG 2 유전자 염기서열 분석에 의한 tiger grouper Epinephelus fuscoguttatus와 giant grouper E. lanceolatus 간 잡종의 동정

  • Kim, Keun-Sik (Department of Life Science and Biotechnology, Soonchunhyang University) ;
  • Lee, Hyo-Ryeon (Department of Life Science and Biotechnology, Soonchunhyang University) ;
  • Sade, Ahemad (Department of Fisheries, Sabah, 4th Floor, Wisma Pertanian Sabah) ;
  • Bang, In-Chul (Department of Life Science and Biotechnology, Soonchunhyang University)
  • 김근식 (순천향대학교 생명시스템학과) ;
  • 이효련 (순천향대학교 생명시스템학과) ;
  • ;
  • 방인철 (순천향대학교 생명시스템학과)
  • Received : 2014.03.06
  • Accepted : 2014.03.23
  • Published : 2014.03.31
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Abstract

Interspecific hybrids between tiger grouper Epinephelus fuscoguttatus and giant grouper E. lanceolatus were genetically identified based on the partial sequence analysis of mitochondrial cytochrome c oxidase I (COX I) gene and nuclear recombination activating gene 2 (RAG 2) gene. Out of 585 base positions of RAG 2, a total of five nucleotide substitutions were detected between the two parental species (E. fuscoguttatus and E. lanceolatus). The hybrids had two distinct types of RAG 2 sequences corresponding to those of both parental species. Mitochondrial COX I gene sequencing showed that hybrids had sequences identical to E. fuscoguttatus. Molecular data clearly demonstrate that hybridization does occur between E. fuscoguttatus and E. lanceolatus, but with E. fuscoguttatus as the maternal parent.

Keywords

References

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