Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Inhibitory Effects of Complex of Mulberry Extract on Degenerative Arthritis

상심자추출물 등 복합물의 퇴행성관절염 억제효과

  • 서형호 ((주)벤스랩 중앙연구소) ;
  • 정종문 (수원대학교 생명과학과)
  • Received : 2014.02.17
  • Accepted : 2014.06.13
  • Published : 2014.08.30
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Abstract

Complex of mulberry extract (CME) is composed of extracts of mulberry (Morus alba L.) fruit, mulberry leaves and black beans (Glycine max (L.) Merr.). In this study, we investigated prevention effects of CME on degenerative arthritis. The $SC_{50}$ value of DPPH radical scavenging by CME was $158.49{\pm}11.35{\mu}/mL$. We found that CME significantly reduced the production of nitric oxide (NO) and protein expression of cyclooxygenase-2 (COX-2) in RAW 264.7 cells which were activated by LPS. Experiments using animal model of degenerative arthritis showed that CME (400 mg/kg body weight) inhibited the production of TNF-${\alpha}$ (77.5%) and IL-$1{\beta}$ (95.0%). Furthermore, it was observed that CME reduced to 85.9% of paw edema induced by carrageenan. These results suggest that CME could improve degenerative arthritis.

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References

  1. Anandakumar S, Joseph JA, Bethapudi B, Agarwal A and Jung EB. (2014). Anti-inflammatory effects of turmeric (Curcuma longa L.) extract on acute and chronic inflammation models. Journal of the Korean Society of Food Science and Nutrition. 43:612-617. https://doi.org/10.3746/jkfn.2014.43.4.612
  2. Bae HA, Baek H, Park HI, Choung MG, Sohn EH, Kim SH, Kim DS, Chung IM, Seong ES, Yu CY and Lim JD. (2011). Effect of fermentation time on the chemical composition of mulberry(Morus alba L.) leaf teas. Korean Journal of Medicinal Crop Science. 19:276-286. https://doi.org/10.7783/KJMCS.2011.19.4.276
  3. Bae MJ and Ye EJ. (2010). Antioxidant activity and in vitro for anticancer effects of manufactured fermented mulberry leaf tea. Journal of the Korean Society of Food Science and Nutrition. 39:796-804. https://doi.org/10.3746/jkfn.2010.39.6.796
  4. Bao Y, Yang Y, Wang L, Gao L, Jiang W, Wang L, Si S and Hong B. (2009). Identification of trichostatin A as a novel transcriptional up-regulator of scavenger receptor BI both in HepG2 and RAW 264.7 cells. Atherosclerosis. 204:127-135. https://doi.org/10.1016/j.atherosclerosis.2008.08.041
  5. Bdouet L, Pascale F, Bonneau M, Wassef M and Laurent A. (2011). In vitro evaluation of (S)-ibuprofen toxicity on joint cells and explants of cartilage and synovial membrane. Toxicology in Vitro. 25:1944-1952. https://doi.org/10.1016/j.tiv.2011.06.018
  6. Boer TN, Huisman AM, Polak AA, Niehoff AG, Rinsum AC, Saris D, Bijlsma JWJ, Lafeber FJPG and Mastbergen SC. (2009). The chondroprotective effect of selective COX-2 inhibition in osteoarthritis: Ex vivo evaluation of human cartilage tissue after in vivo treatment. Osteoarthritis and Cartilage. 17:482-488. https://doi.org/10.1016/j.joca.2008.09.002
  7. Blios MS. (1959). Antioxidant determination by the use of a stable free radical. Nature 181:1199-1200.
  8. Botting RM. (2006). Inhibitors of cyclooxygenases: Mechanisms, selectivity and uses. Journal of Physiology and Pharmacology. 5:113-124.
  9. Carey MA, Germolec DR, Langenbach R and Zeldin DC. (2003). Cyclooxygenase enzymes in allergic inflammation and asthma. Prostaglandins, Leukotrienes and Essential Fatty Acids. 69:157-162. https://doi.org/10.1016/S0952-3278(03)00076-0
  10. Castaneda S, Blas JAR, Largo R and Beaumont GH. (2012). Subchondral bone as a key target for osteoarthritis treatment. Biochemical Pharmacology. 83:315-323. https://doi.org/10.1016/j.bcp.2011.09.018
  11. Chang MC, Hung SC, Chen YK, Chen TL, Lee CF, Lee HC, Wang KL, Chiou CC and Wei YH. (2005). Accumulation of mitochondrial DNA with 4977-bp deletion in knee cartilage e an association with idiopathic osteoarthritis. Osteoarthritis and Cartilage. 13:1004-1011. https://doi.org/10.1016/j.joca.2005.06.011
  12. Dinarello CA. (2000). Proinflammatory cytokines. Chest. 118:503-508. https://doi.org/10.1378/chest.118.2.503
  13. Feghali CA and Wright TM. (1997). Cytokines in acut and chronic inflammation. Frontiers in Bioscience. 2:12-26.
  14. Janusz MJ, Hookfin EB, Heitmeyer SA, Woessner JF, Freemont AJ, Hoyland JA, Brown KK, Hsieh LC, Almstead NG, De B, Natchus MG, Pikul S and Taiwo YO. (2001). Moderation of iodoacetate-induced experimental osteoarthritis in rats by matrix metalloproteinase inhibitors. Osteoarthritis and Cartilage. 9:751-760. https://doi.org/10.1053/joca.2001.0472
  15. Jeon YH, Won JH, Kwon JE and Kim MR. (2011). Antioxidant activity and cytotoxic effect of an ethanol extract from Seoritae. Korean Journal of Food and Cookery Science. 27:1-10. https://doi.org/10.9724/kfcs.2011.27.3.001
  16. Katsube T, Tsurunaga Y, Sugiyama M, Furuno T and Yamasaki Y. (2009). Effect of air-drying temperature on antioxidant capacity and stability of polyphenolic compounds in mulberry(Morus alba L.) leaves. Food Chemistry. 113:964-969. https://doi.org/10.1016/j.foodchem.2008.08.041
  17. Kim EO, Lee YJ, Leem HH, Seo IH, Yu MH, Kang DH and Choi SW. (2010). Comparison of nutritional and functional constituents, and physicochemical characteristics of mulberry from seven different Morus alba L. cultivars. Journal of the Korean Society of Food Science and Nutrition. 39:1467-1475. https://doi.org/10.3746/jkfn.2010.39.10.1467
  18. Kim HB, Kim AJ and Kim SY. (2003a). The analysis of functional materials in mulberry fruit and food product development trends. Food Science and Industry. 36:49-60.
  19. Kim HJ, Kim JK, Whang WK, Ham IH and Kwon SH. (2003b). Effects of Mori folium and Crataegus pinnatifida leave extracts on CCl4-induced hepatotoxicity in rats. Yakhak Hoeji. 47:2006-2011.
  20. Kim OG and Jeong JC. (2006). Effects of Mori folium extract in streptozotocin-induced diabetic rats. Journal of Korean Oriental Internal Medicine. 27:811-821.
  21. Kim TW, Kwon YB, Lee JH, Yang IS, Youm JK, Lee HS and Moon JY. (1996). A study on the antidiabetic effect of mulberry fruits. Journal of Sericultural and Entomological Science. 38:100-107.
  22. Kim YH, Lee JH, Lee YS and Yun HT. (2006). Antioxidant activity and extraction efficiency of anthocyanin pigments in black colored soybean. Korean Soybean Digest. 23:1-9.
  23. Korhonen R, Lahti A, Kankaanranta H and Moilanen E. (2005). Nitric oxide production and signaling in inflammation. Current Drug Targets-Inflammation & Allergy. 4:471-479. https://doi.org/10.2174/1568010054526359
  24. Kraan PM and Berg WB. (2008). Osteoarthritis in the context of ageing and evolution loss of chondrocyte differentiation block during ageing. Ageing Research Reviews. 7:106-113. https://doi.org/10.1016/j.arr.2007.10.001
  25. Kwak HY, Lee SJ, Lee DY, Jung L, Bae NH, Hong SY, Kim GW and Baek NI. (2008). Cytotoxic and anti-inflammatory activities of lipids from the Nuruk(Rhizopus oryzae KSD-815). Journal of the Korean Society for Applied Biological Chemistry. 51:142-147.
  26. Laine L, White WB, Rostom A and Hochberg M. (2008). COX- 2 selective inhibitors in the treatment of osteoarthritis. Seminars in Arthritis and Rheumatism. 38:165-187. https://doi.org/10.1016/j.semarthrit.2007.10.004
  27. Lee EJ and Bae JH. (2011). Study on the alleviation of an alcohol induced hangover and the antioxidant activity by mulberry fruit. Korean Journal of Food And Nutrition. 24:204-209. https://doi.org/10.9799/ksfan.2011.24.2.204
  28. Lee JS. (2013). Isolation, characterization, and immune stimulating activity of a water-soluble polysaccharide purified from Korean mulberry fruits. Master Thesis. Catholic University of Daegu. p.1-96.
  29. Lee SE, Lee JH, Kim JK, Kim GS, Kim YO, Soe JS, Choi JH, Lee ES, Noh HJ and Kim SY. (2011). Anti-inflammatory activity of medicinal plant extracts. Korean Journal of Medicinal Crop Science. 19:217-226. https://doi.org/10.7783/KJMCS.2011.19.4.217
  30. Lee SH and Kim GH. (2008). Inhibitory effect of mulberry extracts on angiogenesis in porcine artery endotherial cells. Journal of Life Sciences. 18:653-659.
  31. Mandhane SN, Shah JH and Thennati R. (2011). Allergic rhinitis: An update on disease, present treatments and future prospects. International Immunopharmacology. 11:1646-1662. https://doi.org/10.1016/j.intimp.2011.07.005
  32. Oh HS, Park YH and Kim JH. (2002). Isoflavone contents, antioxidative and fibrinolytic activities of some commercial cooking-with-race soybeans. Korean Journal of Food Science and Technology. 34:498-504.
  33. Park E, Lee SM, Lee JE and Kim JH. (2013). Antiinflammatory activity of mulberry leaf extract through inhibiton of NF-${\kappa}B$. Journal of Functional Foods. 5:178-186. https://doi.org/10.1016/j.jff.2012.10.002
  34. Park SW, Jung YS and Ko KC. (1997). Quantitative analysis of anthocyanins among mulberry cultivars and their pharmacological screening. Journal of the Korean Society for Horticultural Science. 38:722-724.
  35. Park SY. (2009). Whitening effects of Mori ramulus, Mori cortex radicis and Mori folium herbal-acupuncture solution after fermentation and heating. Master Thesis. Daegu Haany University. p.1-37.
  36. Pecchi E, Dallaporta M, Jean A, Thirion S and Troadec JD. (2009). Prostaglandins and sickness behavior: Old story, new insights. Physiology & Behavior. 97:279-292. https://doi.org/10.1016/j.physbeh.2009.02.040
  37. Ryu IH and Kwon TO. (2012). Sensory characteristics of granular tea and the components of mulberry fruit extracts by different extraction process. Korean Journal of Medicinal Crop Science. 20:331-338. https://doi.org/10.7783/KJMCS.2012.20.5.331
  38. Shin HC, Sung HS, Lee YS and Sohn HS. (2001). Nutritional adequacy and beneficial effects of soy formula. Korea Soybean Digest. 18:10-25.
  39. Sladowski D, Steer SJ, Clothier RH and Balls M. (1993). An improved MTT assay. Journal of Immunological Methods. 157:203-207. https://doi.org/10.1016/0022-1759(93)90088-O
  40. Sud'ina GF, Pushkareva MA, Shephard P and Klein T. (2008). Cyclooxygenase(COX) and 5-lipoxygenase(5-LOX) selectivity of COX inhibitors. Prostaglandins, Leukotrienes and Essential Fatty Acids. 78:99-108. https://doi.org/10.1016/j.plefa.2007.12.006
  41. Vane J and Botting R. (1987). Inflammation and Mechanism of action of antiinflammatory drugs. FASEB Journal. 1:89-96. https://doi.org/10.1096/fasebj.1.2.3111928
  42. Winter CA, Riskey EA and Nuss GW. (1962). Carrageenininduced edema in hind paw of the tat as an assay for antiinflammatory drugs. Proceedings of the Society for Experimental Biology and Medicine. 111:544-547. https://doi.org/10.3181/00379727-111-27849
  43. Yeom HE. (2013). Symptoms, aging-stereotyped beliefs, and health-promoting behaviors of older women with and without osteoarthritis. Geriatric Nursing. 20:1-7.

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