Journal of fish pathology (한국어병학회지)

The Korean Society of Fish Pathology (한국어병학회)
권호 표지
DOI QR코드

DOI QR Code

Inhibitory Effects of Candidate Probiotic Bacteria on the Growth of Fish Pathogenic Bacteria, Streptococcus sp.

Candidate Probiotic Bacteria의 어류병원성 Streptococcus sp. 성장에 대한 억제 효과

  • Lee, Minyeong (International Commerce and Trade Division, Ministry of Oceans and Fisheries) ;
  • Kim, Eunheui (Department of Aqualife Medicine, Chonnam National University)
  • 이민영 (해양수산부 통상무역협력과) ;
  • 김은희 (전남대학교 수산생명의학과)
  • Received : 2014.07.30
  • Accepted : 2014.08.20
  • Published : 2014.08.30
Download PDF
⟨ Previous Next ⟩

Abstract

For the treatments and protection of bacterial fish disease, many requirements are needed for aquatic probiotics so that they are effective in aquaculture animals but are also harmless to humans. In the present study, among 17 candidate probiotic bacteria (CPB) obtained from the edible part of the shellfish, Bacillus sp. CPB-St (CPB-St) were selected and in vitro evaluated for the possibility as a probiotic strain for the control of fish streptococcosis which frequently occurs in the olive flounder farms. CPB-St showed inhibitory effects on the growth of various fish pathogenic bacteria, Streptococcus sp., S. parauberis, S. iniae, Lactococcus garvieae and L. piscium by the double layer test ranging about 18~26 mm of clear zone. Inhibitory activity of CPB-St to Streptococcus sp. was observed 6 hours after and the growth of Streptococcus sp. was decreased to 8~55 folds in the co-culture of CPB-St with Streptococcus sp.. The safety of CPB-St to fish and survival of CPB-St in the intestine were assessed in the olive flounder, Paralichthys olivaceus. Fish mortality was not observed in artificial infection with CPB-St for 2 weeks. CPB-St was entirely excreted from the stomach and intestine 24 hours after oral injection. This results indicate that CPB-St has potential applications as a probiotic for the control of fish streptococcosis in aquaculture.

세균성 어류질병의 치료 및 예방을 위하여 어류와 인체에 안전한 수산용 probiotic의 개발이 요구되고 있다. 본 연구에서는 남해안 일대에서 양식되고 있는 참굴 (Crassostrea gigas), 바지락 (Ruditapes philippinarum), 피조개 (Scapharca broughtonii), 새조개 (Fulvia mutica)의 가식 부위로부터 17균주의 candidate probiotic bacteria (CPB)를 분리하였다. 나아가 다양한 연쇄상구균 (Lactococcus garvieae, L. piscium, Streptococcus sp., S. iniae, and S. parauberis)에 대하여 강한 생장 억제력을 보이는 균주(CPB-St)를 선별하여 어류의 연쇄구균증 관리를 위한 probiotic 균주로서의 개발 가능성을 알아보았다. CPB-St 균주를 double layer test를 통하여 다양한 연쇄상구균들에 대한 생장 억제 정도를 알아보았으며, 혼합 배양에서의 Streptococcus sp.에 대한 생장 억제 능력을 확인하였고, CPB-St의 어류에 대한 안전성 및 장내 생존 유무를 평가하였다. CPB-St는 대부분의 연쇄상구균에 대하여 18~26 mm의 생장 억제대를 형성함으로써 높은 생장 억제 능력을 보였다. CPB-St와의 혼합 배양에서 Streptococcus sp.는 6시간째부터 생장 억제 현상이 관찰되기 시작하여 12시간 전후로 8~55배 정도의 감소를 나타내었다. CPB-St의 어류에 대한 병원성 유 무를 알아본 결과, 2주일 동안 CPB-St로 인한 어류 폐사는 관찰되지 않았다. CPB-St의 1회 강제 경구 투여 후, 위와 장에서 CPB-St의 생존을 확인한 결과 투여 24시간 후에는 CPB-St가 모두 배출되는 것으로 나타났다. CPB-St를 probiotic bacteria로 개발하기 위해서는 어류의 장내 정착 가능성과 먹이를 통한 효과의 정확한 검증이 추후 이루어져야 할 것이다.

Keywords

References

  1. Austin, B. and Austin, D.A.: Bacterial fish pathogens, pp17-57, 5th ed., Springer Dordrecht Heiderberg, London UK, 2012.
  2. Balcazar, J.L., de Blas, I., Ruiz-Zarzuela, I., Cunningham, D., Vendrell, D. and Muzquiz, J.L.: The role of probiotics in aquaculture. Veterinary Microbiology, 114: 173-186, 2006. https://doi.org/10.1016/j.vetmic.2006.01.009
  3. Cha, J.H., Yang, S.Y., Woo, S.H., Song, J.W., Oh, D.H. and Lee, K.J.: Effects of dietary supplementation with Bacillus sp. on growth performance, feed utilization, innate immunity and disease resistance against Streptococcus iniae in olive flounder, Paralichthys olivaceus. Kor J Fish Aquat Sci, 45: 35-42, 2012. https://doi.org/10.5657/KFAS.2012.0035
  4. Crab, R., Defoirdt, T., Bossier, P. and Verstraete, W.: Biofloc technology in aquaculture: Beneficial effects and future challenges. Aquaculture, 356-357: 351-356, 2012. https://doi.org/10.1016/j.aquaculture.2012.04.046
  5. Fernandez, M.F., Boris, S. and Barbės, C.: Probiotic properties of human lactobacilli strains to be used in the gastrointestinal tract. Journal of Applied Microbiology, 94: 449-455, 2003. https://doi.org/10.1046/j.1365-2672.2003.01850.x
  6. Gatesoupe, F.J.: The use of probiotics in aquaculture. Aquaculture, 180: 147-165, 1999. https://doi.org/10.1016/S0044-8486(99)00187-8
  7. Gomez-Gil, B., Roque, A. and Turnbull, J.F.: The use and selection of probiotic bacteria for use in the culture of larval aquatic organisms. Aquaculture, 191: 259-270, 2000. https://doi.org/10.1016/S0044-8486(00)00431-2
  8. Gram, L., Melchiorsen, J., Spanggaard, B., Huber, I. and Nielsen, T.F.: Inhibition of Vibrio anguillarum by Pseudomonas fluorescens AH2, a possible probiotic treatment of fish. Appl. Environ. Microbiol. 65: 969-973, 1999.
  9. Irianto, A. and Austin, B.: Use of probiotics to control furunculosis in rainbow trout, Oncorhynchus mykiss (Walbaum). Journal of Fish Diseases, 25: 333- 342, 2002. https://doi.org/10.1046/j.1365-2761.2002.00375.x
  10. Kesarcodi-Watson, A., Kaspar, H., Lategan, M.J., Gibson, L.: Probiotics in aquaculture: The need, principles and mechanisms of action and screening processes. Aquaculture, 274: 1-14, 2008. https://doi.org/10.1016/j.aquaculture.2007.11.019
  11. Nwachi, O.F.: An overview of the importance of probiotics in aquaculture. J. fish. Aquat. Sci., 8: 30-32, 2013. https://doi.org/10.3923/jfas.2013.30.32
  12. Ringo, E. and Gatesoupe, F.: Lactic acid bacteria in fish: a review. Aquaculture, 160: 177-203, 1998. https://doi.org/10.1016/S0044-8486(97)00299-8
  13. Sugita, H., Hirose, Y., Matsuo, N. and Deguchi, Y.: Production of the antibacterial substance by Bacillus sp. strain NM 12, an intestinal bacterium of Japanese coastal fish. Aquaculture, 165: 269-280, 1998. https://doi.org/10.1016/S0044-8486(98)00267-1
  14. Tuan, T.N., Duc, P.M. and Hatai, K.: Overview of the use of probiotics in aquaculture. International Journal of Research in Fisheries and Aquaculture, 3: 89-97, 2013.
  15. van der Waaij, D. and Nord, C.E.: Development and persistence of multi-resistance to antibiotics in bacteria: an analysis and a new approach to this urgent problem. International Journal of Antimicrobial Agents, 16: 191-197, 2000. https://doi.org/10.1016/S0924-8579(00)00227-2
  16. Verschuere, L., Rombaut, G., Sorgeloos, P. and Verstraete, W.: Probiotic bacteria as biological control agents in aquaculture. Microbiology and molecular biology reviews, 64: 655-671, 2000. https://doi.org/10.1128/MMBR.64.4.655-671.2000
  17. Yang, B.G., Jun, Y.J. and Heo, M.S.: Screening and characterization of probiotic strains for prevention of bacterial fish disease. Kor. J. Microbiol. Biotechnol., 31: 129-134, 2003.

Cited by

  1. Biological Characters of Bacillus pumilus CPB-St Inhibiting the Growth of Fish Pathogenic Streptococci vol.28, pp.2, 2015, https://doi.org/10.7847/jfp.2015.28.2.063