Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Effect of LEDs on shoot multiplication and rooting of rare plant Abeliophyllum distichum Nakai

희귀 수종 미선나무(Abeliophyllum distichum Nakai.)의 기내 증식 및 발근에 미치는 LED (light emitting diode) 효과

  • Lee, Na Nyum (Division of Biotechnology, Korea Forest Research Institute) ;
  • Choi, Yong Eui (Depart. of Forestry, Kangwon Nat'l University) ;
  • Moon, Heung Kyu (Division of Biotechnology, Korea Forest Research Institute)
  • 이나념 (국립산림과학원 산림생명공학과) ;
  • 최용의 (강원대학교 산림자원학과) ;
  • 문흥규 (국립산림과학원 산림생명공학과)
  • Received : 2014.06.11
  • Accepted : 2014.06.25
  • Published : 2014.06.30
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Abstract

This study was conducted to elucidate the effect of light sources and explant types on in vitro shoot multiplication and rooting of a rare and endangered plant Abeliophyllum distichum. Both apical buds and axillary buds were used as explants under 4 different light sources, cool white florescent light (F), 100% blue light-emitting diode (LED) (B), 50% blue and 50% red LED mixture (BR), and 100% red LED (R). Clear difference was observed in terms of shoot proliferation by light sources types but not by position-dependent explant types. Multiple shoot induction rates were enhanced under both B and BR light sources. Spontaneous rooting was induced in shoot induction medium under B light source. Both the rates of rooting and numbers of roots per explant were higher in apical bud explants compared to axillary bud explants. Interestingly R light source stimulated shoot elongation but inhibited root development. Therefore, our results suggest that the use of apical bud explants under B or BR light sources is suitable for in vitro micropropagation of a rare and endangered plant species, Abeliophyllum distichum.

희귀 멸종위기 식물인 미선나무를 재료로 기내 증식 및 발근에 미치는 광질 및 절편체(정아지 및 액아지) 효과를 조사하였다. 광질은 냉백색 형광등(F), 100% 청색광 LED(B), 혼합광(BR; 50% blue LED+50% red LED) 및 100% 적색광(R)을 사용하였다. 줄기의 증식은 절편체 종류에 따른 뚜렷한 차이를 보이지 않았으나 광질에 따른 차이를 보였다. 전반적으로 줄기의 증식은 청색광 및 혼합광에서 촉진되는 것으로 나타났으며, 특히 청색광에서는 일부 절편에서 발근이 이루어 졌다. 줄기의 생장 역시 광질에 따른 현저한 차이가 없었다. 적색광에서 줄기 신장이 촉진된 반면 잎의 발달은 저조하였다. 발근은 전반적으로 광질에 관계없이 정아지가 액아지보다 양호하였고, 뿌리수도 증가하였으나 혼합광에서는 발근이 억제되었다. 한편 적색광에서는 뿌리의 발달이 매우 부진하여 다른 광질과 유의적인 차이를 보였다. 발근유도 시 줄기는 주로 단일 줄기로 자랐다. 이상의 결과를 볼 때 LED를 이용한 미선나무의 기내번식은 정아지를 절편으로 청색광 및 혼합광 LED (BR) 하에서 배양함이 좋은 것으로 나타났다.

Keywords

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