Nutrition Research and Practice

The Korean Nutrition Society (한국영양학회)
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The effect of long-term supplementation with different dietary ${\omega}-6/{\omega}-3$ ratios on mineral content and ex vivo prostaglandin E2 release in bone of growing rabbits

  • Alnouri, Doha Mustafa (Department of Food Sciences and Nutrition, Faculty of Food Sciences and Agriculture, King Saud University) ;
  • El-Din, Mohamed Fekry Serag (Department of Food Sciences and Nutrition, Faculty of Food Sciences and Agriculture, King Saud University) ;
  • Al-Khalifa, Abdulrhman Salih (Department of Food Sciences and Nutrition, Faculty of Food Sciences and Agriculture, King Saud University)
  • Received : 2013.10.29
  • Accepted : 2014.03.06
  • Published : 2014.08.01
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Abstract

BACKGROUND/OBJECTIVES: The aim of this research was to study the different long term effects of consumption of dietary oil sources with varying omega-6/omega-3 (${\omega}-6/{\omega}-3$) polyunsaturated fatty acids (PUFAs) ratios on bone marrow fatty acid level, ex vivo prostaglandin $E_2$ ($PGE_2$) release, and mineral content of bone in rabbits. MATERIALS/METHODS: For this purpose, weaning and female New Zealand white rabbits were purchased and randomly divided into five groups and offered ad libitum diets containing 70 g/kg of added oil for 100 days. The dietary lipid treatments were formulated to provide the following ratios of ${\omega}-6/{\omega}-3$ fatty acids: 8.68 soy bean oil (SBO control), 21.75 sesame oil (SO), 0.39 fish oil (FO), 0.63 algae oil (DHA), and 0.68 algae oils (DHA/ARA). DHA and ARA are two types of marine microalgae of the genus Crypthecodinium cohnii. RESULTS: The dietary treatments had significant effects on the bone marrow fatty acids of rabbits. Rabbits fed the FO diet, containing the highest ${\omega}-3$ PUFA concentration, and those fed the SBO diet showed the highest ${\omega}-6$ PUFA. On the other hand, a positive correlation was observed between Ex vivo $PGE_2$ level and the ${\omega}-6/{\omega}-3$ dietary ratio. Significant effects of dietary treatment on femur Ca, P, Mg, and Zn contents were observed in both genders. CONCLUSIONS: Findings of the current study clearly demonstrated that dietary PUFA, particularly ${\omega}-6/{\omega}-3$ and ARA/EPA ratios are important factors in determining bone marrow fatty acid profile, and this in turn determines the capacity of bone for synthesis of $PGE_2$, thereby reducing bone resorption and improving bone mass during growth.

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References

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