Asian Pacific Journal of Cancer Prevention

Asian Pacific Journal of Cancer Prevention (아시아태평양암예방학회)
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Role of TGF-β1 in Human Colorectal Cancer and Effects after Cantharidinate Intervention

  • Ma, Jie (Department of Molecular Medicine, Jilin University) ;
  • Gao, Hai-Mei (Department of Medical Records, the Second Hospital of Jilin University) ;
  • Hua, Xin (Department of cerebral surgery, the Third Hospital of Jilin University) ;
  • Lu, Ze-Yuan (Department of Clinical Pharmacy and Pharmaceutical Management, Jilin University) ;
  • Gao, Hai-Cheng (Department of Clinical Pharmacy and Pharmaceutical Management, Jilin University)
  • Published : 2014.05.15
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Abstract

Effects of transforming growth factor-beta (TGF-${\beta}$) were investigated in human colorectal cancer, and the influence of cantharidinate in inhibiting TGF-${\beta}1$ expression was explored. Relationships among TGF-${\beta}1$ and sex, age, tumor size, tumor location, tumor stage were also analyzed. H&E and immunohistochemistry staining were employed to assess colorectal cancer and TGF-${\beta}1$ expression, respectively. Then, HCT-116 CRC cells were randomly divided into four groups, controls, no serum-treated, chemotherapy and cantharidinate-treated. Immunohistochemistry and real-time PCR were employed to assess the expression of TGF-${\beta}1$ in CRC cells. Our data showed that the expression of TGF-${\beta}1$ might be associated with tumor size and tumor location (P<0.05). The expression of TGF-${\beta}1$ in CRC groups was higher than in adjacent groups (P<0.05). In addition, the expression of TGF-${\beta}1$ in cantharidinate-treated group was much lower than in CRC group (P<0.05). Taken together, these results suggest that TGF-${\beta}1$ plays an important role in CRC development. Cantharidinate might inhibit the expression of TGF-${\beta}1$ and control the development of colorectal cancer.

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