Asian Pacific Journal of Cancer Prevention

Asian Pacific Journal of Cancer Prevention (아시아태평양암예방학회)
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Metabolomics Investigation of Cutaneous T Cell Lymphoma Based on UHPLC-QTOF/MS

  • Zhou, Qing-Yuan (School of Pharmacy, Fudan University) ;
  • Wang, Yue-Lin (School of Pharmacy, Fudan University) ;
  • Li, Xia (School of Pharmacy, Fudan University) ;
  • Shen, Xiao-Yan (Department of Dermatology, Ruijin Hospital, School of Medicine, Shanghai Jiaotong University) ;
  • Li, Ke-Jia (Department of Dermatology, Ruijin Hospital, School of Medicine, Shanghai Jiaotong University) ;
  • Zheng, Jie (Department of Dermatology, Ruijin Hospital, School of Medicine, Shanghai Jiaotong University) ;
  • Yu, Yun-Qiu (School of Pharmacy, Fudan University)
  • Published : 2014.07.15
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Abstract

Objectives: The identification of cutaneous T cell lymphoma (CTCL) biomarkers may serve as a predictor of disease progression and treatment response. The aim of this study was to map potential biomarkers in CTCL plasma. Design and Methods: Plasma metabolic perturbations between CTCL cases and healthy individuals were investigated using metabolomics and ultrahigh performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UHPLC-QTOF/MS). Results: Principal component analysis (PCA) of the spectra showed clear metabolic changes between the two groups. Thirty six potential biomarkers associated with CTCL were found. Conclusions: Based on PCA, several biomarkers were determined and further identified by LC/MS/MS analysis. All of these could be potential early markers of CTCL. In addition, we established that heparin as a nticoagulant has better pre-treatment results than EDTA with the UHPLC-QTOF/MS appraoch.

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References

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