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Inhibition of mouse SP2/0 myeloma cell growth by the B7-H4 protein vaccine

  • Mu, Nan (State Key Laboratory of Cancer Biology, Biotechnology Center, School of Pharmacy) ;
  • Liu, Nannan (Experiment Teaching Center of Basic Medicine, The Fourth Military Medical University) ;
  • Hao, Qiang (State Key Laboratory of Cancer Biology, Biotechnology Center, School of Pharmacy) ;
  • Xu, Yujin (State Key Laboratory of Cancer Biology, Biotechnology Center, School of Pharmacy) ;
  • Li, Jialin (State Key Laboratory of Cancer Biology, Biotechnology Center, School of Pharmacy) ;
  • Li, Weina (State Key Laboratory of Cancer Biology, Biotechnology Center, School of Pharmacy) ;
  • Wu, Shouzhen (State Key Laboratory of Cancer Biology, Biotechnology Center, School of Pharmacy) ;
  • Zhang, Cun (State Key Laboratory of Cancer Biology, Biotechnology Center, School of Pharmacy) ;
  • Su, Haichuan (Department of Oncology, Tangdu Hospital, Fourth Military Medical University)
  • Received : 2013.07.22
  • Accepted : 2013.11.13
  • Published : 2014.07.31

Abstract

B7-H4 is a member of B7 family of co-inhibitory molecules and B7-H4 protein is found to be overexpressed in many human cancers and which is usually associated with poor survival. In this study, we developed a therapeutic vaccine made from a fusion protein composed of a tetanus toxoid (TT) T-helper cell epitope and human B7-H4IgV domain (TT-rhB7-H4IgV). We investigated the anti-tumor effect of the TT-rhB7-H4IgV vaccine in BALB/c mice and SP2/0 myeloma growth was significantly suppressed in mice. The TT-rhB7-H4IgV vaccine induced high-titer specific antibodies in mice. Further, the antibodies induced by TT-rhB7-H4IgV vaccine were capable of depleting SP2/0 cells through complement-dependent cytotoxicity (CDC) in vitro. On the other hand, the poor cellular immune response was irrelevant to the therapeutic efficacy. These results indicate that the recombinant TT-rhB7-H4IgV vaccine might be a useful candidate of immunotherapy for the treatment of some tumors associated with abnormal expression of B7-H4.

Acknowledgement

Supported by : National Natural Foundation of China

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