Journal of Embryo Transfer (한국수정란이식학회지)

The Korean Society of Embryo Transfer (한국수정란이식학회)
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Determination of the Granulosa Cell-Specific Endothelin Receptor A Deletion on Ovarian Function

  • Cho, Jong-Ki (College and Research Institute of Veterinary Medicine, Chungnam National University)
  • Received : 2014.05.20
  • Accepted : 2014.06.16
  • Published : 2014.06.30
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Abstract

Endothelin 2 (EDN2) induces follicular rupture by constricting periovulatory follicles. In this study, it was investigated the mechanisms of EDN2 action on follicular rupture with respect of receptor using the conditionally granulosa cell specific EDN2 receptor type A (ETa) KO mice (gcETaKO; $ETa^{flox/-}{\cdot}Amhr2^{Cre}$). It was generated the gcETaKO mice by breeding with $ETa^{flox/-}$ mice after mono-alleic ETa knockout by $ZP3^{Cre}$ and $Amhr2^{Cre}$ mice. Fertility, ovulation and maturation rates of ovulated oocytes after super ovulation were investigated in the gcETaKO mice compared with wild-type mice ($ETa^{flox/flox}$ and $ETa^{flox/-}$) as a control group. In the gcETaKO mice, normal fertility after breeding with male mice was shown compared with wild-type mice. And, there was no significant differences in ovulation rates after super ovulation, however its maturation rates was lower than that of wild type mice. These findings show that EDN2 in follicular rupture for ovulation is related with an other ETa not in granulosa cells. Further studies are needed to investigate how EDN2 is acted in ovarian follicular rupture for ovulation.

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References

  1. Al-Alem L, Bridges PJ, Su W, Gong MC, Iglarz M and Ko C. 2007. Endothelin-2 induces oviductal contraction via endothelin receptor subtype A in rats. The Journal of Endocrinology 193: 383-391. https://doi.org/10.1677/JOE-07-0089
  2. Arango NA, Kobayashi A, Wang Y, Jamin SP, Lee HH, et al. 2008. A mesenchymal perspective of Mullerian duct differentiation and regression in Amhr2-lacZ mice. Molecular Reproduction and Development 75: 1154-1162. https://doi.org/10.1002/mrd.20858
  3. Bagchi A and Ghosh TC. 2006. Structural insight into the interactions of SoxV, SoxW and SoxS in the process of transport of reductants during sulfur oxidation by the novel global sulfur oxidation reaction cycle. Biophys. Chem. 119: 7-13. https://doi.org/10.1016/j.bpc.2005.08.011
  4. Boyer A, Yeh JR, Zhang X, Paquet M, Gaudin A, et al. 2012. CTNNB1 signaling in sertoli cells downregulates spermatogonial stem cell activity via WNT4. PloS one 7: e29764. https://doi.org/10.1371/journal.pone.0029764
  5. Bridges PJ, Jo M, Al Alem L, Na G, Su W, et al. 2010. Production and binding of endothelin-2 (EDN2) in the rat ovary: endothelin receptor subtype A (EDNRA)-mediated contraction. Reproduction, Fertility, and Development 22: 780-787. https://doi.org/10.1071/RD09194
  6. Cho J, Kim H, Kang DW, Yanagisawa M and Ko C. 2012. Endothelin B receptor is not required but necessary for finite regulation of ovulation. Life Sciences.
  7. Clouthier DE, Hosoda K, Richardson JA, Williams SC, Yanagisawa H, et al. 1998. Cranial and cardiac neural crest defects in endothelin-A receptor-deficient mice. Development 125: 813-824.
  8. Griswold MD. 1988. Protein secretions of Sertoli cells. International Review of Cytology 110: 133-156. https://doi.org/10.1016/S0074-7696(08)61849-5
  9. Hernandez Gifford JA, Hunzicker-Dunn ME and Nilson JH. 2009. Conditional deletion of beta-catenin mediated by Amhr2cre in mice causes female infertility. Biology of Reproduction 80: 1282-1292. https://doi.org/10.1095/biolreprod.108.072280
  10. Jegou B. 1993. The Sertoli-germ cell communication network in mammals. International Review of Cytology 147: 25-96. https://doi.org/10.1016/S0074-7696(08)60766-4
  11. Jorgez CJ, Klysik M, Jamin SP, Behringer RR and Matzuk MM. 2004. Granulosa cell-specific inactivation of follistatin causes female fertility defects. Mol. Endocrinol. 18: 953-967. https://doi.org/10.1210/me.2003-0301
  12. Kawamura K, Ye Y, Liang CG, Kawamura N, Gelpke MS, et al. 2009. Paracrine regulation of the resumption of oocyte meiosis by endothelin-1. Developmental Biology 327: 62-70. https://doi.org/10.1016/j.ydbio.2008.11.033
  13. Kedzierski RM, Grayburn PA, Kisanuki YY, Williams CS, Hammer RE, et al. 2003. Cardiomyocyte-specific endothelin a receptor knockout mice have normal cardiac function and an unaltered hypertrophic response to angiotensin II and isoproterenol. Molecular and Cellular Biology 23: 8226-8232. https://doi.org/10.1128/MCB.23.22.8226-8232.2003
  14. Ko C, Gieske MC, Al-Alem L, Hahn Y, Su W, et al. 2006. Endothelin- 2 in ovarian follicle rupture. Endocrinology 147: 1770-1779. https://doi.org/10.1210/en.2005-1228
  15. Ko C, In YH and Park-Sarge OK. 1999. Role of progesterone receptor activation in pituitary adenylate cyclase activating polypeptide gene expression in rat ovary. Endocrinology 140: 5185-5194. https://doi.org/10.1210/endo.140.11.7149
  16. Kyronlahti A, Euler R, Bielinska M, Schoeller EL, Moley KH, et al. 2011. GATA4 regulates Sertoli cell function and fertility in adult male mice. Molecular and Cellular Endocrinology 333: 85-95. https://doi.org/10.1016/j.mce.2010.12.019
  17. Livak KJ and Schmittgen TD. 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2 (-Delta Delta C(T)) method. Methods 25: 402-408. https://doi.org/10.1006/meth.2001.1262
  18. Palanisamy GS, Cheon YP, Kim J, Kannan A, Li Q, et al. 2006. A novel pathway involving progesterone receptor, endothelin- 2, and endothelin receptor B controls ovulation in mice. Mol. Endocrinol. 20: 2784-2795. https://doi.org/10.1210/me.2006-0093
  19. Tanwar PS, Kaneko-Tarui T, Zhang L, Rani P, Taketo MM, Teixeira J. 2010. Constitutive WNT/beta-catenin signaling in murine Sertoli cells disrupts their differentiation and ability to support spermatogenesis. Biology of Reproduction 82: 422-432. https://doi.org/10.1095/biolreprod.109.079335