Journal of Embryo Transfer (한국수정란이식학회지)

The Korean Society of Embryo Transfer (한국수정란이식학회)
권호 표지
DOI QR코드

DOI QR Code

On the Development of Parthenogenetic Oocytes by Cytochalasin B and Production of Cloned Mice by SCNT

  • Sim, Bo-Woong (Animal Biotechnology, Graduate School of Bio. and Information Technology, Institute of Genetic Engineering, Hankyong National University) ;
  • Min, Kwan-Sik (Animal Biotechnology, Graduate School of Bio. and Information Technology, Institute of Genetic Engineering, Hankyong National University)
  • Received : 2014.03.12
  • Accepted : 2014.06.16
  • Published : 2014.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

This study was conducted to optimize the efficiency of cloning and to produce cloned mice. The majority of cloned mammals derived by nuclear transfer (NT) die during gestation and have enlarged and dysfunctional placentas. In this study, the optimized conditions were established to produce clone mice. The parthenogenetic oocytes were activated after 6 h regardless of cytochalasin B (CB) concentration. CB treatment ($2{\mu}g/ml$) was found second polar body. Lower concentration of CB was decreased the activation rate, but the second polar body was the best highly increased during 6 h incubation. The small fragments were exhibited in the $5{\mu}g/ml$ treatment of CB, but it was not found in lower concentration groups (> $2.5{\mu}g/ml$). To examine effects of $SrCl_2$ on the adult cumulus cells, somatic cell NT oocytes were exposed during 0.5, 1 and 6 hrs. The second polar body was significantly greater in 0.5 h exposure group (6.6%) than 1, 6 hrs. Developmental rate from 2-cell to 4-cell was the lowest in 7.5 mM Strontium chloride ($SrCl_2$) groups (84.1% and 64.3%) than 5, 10 m $MSrCl_2$. The implantation rate was not significantly difference among 5, 7.5 and 10 m $MSrCl_2$ group. Three live fetuses were produced by SCNT. SCNT placentas were remarkably heavier than IVF group (8 fetuses) (0.34, 0.34, 0.33 vs 0.14 g) compared with the placenta weight of IVF and SCNT clones.

Keywords

References

  1. Baguisi A, Behboodi E, Melican DT, Rollock JS and Destrempes MM. 1997. Production of goats by somatic cell nucleus transfer. Nat. Biotechnol. 17: 456-461.
  2. Boiani M, Eckardt S, Leu NA, Scholer HR and McLaughlin KJ 2003. Pluripotency deficit in clones overcome by clone aggregation: epigenetic complementation. EMBO J. 22: 5304-5312. https://doi.org/10.1093/emboj/cdg507
  3. Bos-Mikich A, Swann K and Whittingham DG. 1995. Calcium oscillations and protein synthesis inhibition synergistically activate mouse oocytes. Mol. Reprod. Dev. 41: 84-90. https://doi.org/10.1002/mrd.1080410113
  4. Capecchi MR. 1989. Altering the genome by homologous recombination. Science 244: 1288-1292. https://doi.org/10.1126/science.2660260
  5. Cibelli JB, Stice SL, Golueke PJ, Kane JJ, Jerry J, Blackwell C, Ponce de Leon FA and Robl JM. 1998. Cloned transgenic calves produced from nonquiescent fetal fibroblasts. Science 280: 1256-1258. https://doi.org/10.1126/science.280.5367.1256
  6. Cuthbertson KS, Whittingham DG and Cobbold PH. 1981. $Ca^{2+}$ in creases in exponential phases during mouse oocyte activation. Nature 294: 754-757. https://doi.org/10.1038/294754a0
  7. Dean W, Bowden L, Aitchison A, Klose J, Moore T, Meneses JJ, Reik W and Feil R. 1998. Altered imprinted gene methylation and expression in completely ES cell-derived mouse fetuses: association with aberrant phenotypes. Development 125: 2273-2282.
  8. Dean W, Santos F, Stojkovic M, Zakhartchenko V, Walter J, Wolf E and Reik W. 2001. Conservation of methylation reprogramming in mammalian development: aberrant reprogramming in cloned embryos. Proc. Natl. Acad. Sci. USA 98: 13734-13738. https://doi.org/10.1073/pnas.241522698
  9. Eggan K, Akutsu H, Loring J, Jackson-Grusby L and Klemm M. 2001. Hybrid vigor, fetal overgrowth, and viability of mice derived by nuclear cloning and tetraploid embryo complementation. Proc. Natl. Acad. Sci. USA 98: 6209-6214. https://doi.org/10.1073/pnas.101118898
  10. Kang YK, Koo DB, Park JS, Choi YH, Chung AS, Lee KK and Han YM. 2001. Aberrant methylation of donor genome in cloned bovine embryos. Nat. Genet. 28: 173-177. https://doi.org/10.1038/88903
  11. Kishigami S, Wakayama S, Thuan NV, Ohta H, Mizutani E, Hikichi T, Bui HT, Balbach S, Ogura A, Boiani M and Wakayama T. 2006. Production of cloned mice by somatic cell nuclear transfer. Nat. Protocols 1: 125-138. https://doi.org/10.1038/nprot.2006.21
  12. Fukuda A, Noda Y, Tsukui S. Matsumoto H, Yano and Mori T. 1987. Influence of water quality on in vitro fertilization and embryo development for the mouse. J. In Vitro Fert. Embryo Transfer 4: 40-45. https://doi.org/10.1007/BF01555434
  13. Inoue K, Kohda T, Lee J, Ogonuki N, Mochida K, Noguchi Y, Tanemura K, Kaneko-Ishino T, Ishino F and Ogura A. 2002. Faithful expression of imprinted genes in cloned mice. Science 295: 297. https://doi.org/10.1126/science.295.5553.297
  14. Kato Y, Tani T, Sotomaru Y, Kurokawa K, Kato J, Doguchi H, Yasue H and Tsunoda Y. 1998. Eight calves cloned from somatic cells of a single adult. Science 282: 2095-2098. https://doi.org/10.1126/science.282.5396.2095
  15. Kono T, Obata Y, Wu Q, Niwa K, Ono Y, Yamamoto Y, Park ES, Seo JS and Ogawa H. 2004. Birth of parthenogenetic mice that can develop to adulthood. Nature 428: 860-864. https://doi.org/10.1038/nature02402
  16. Kono T, Sotomaru Y, Sato Y and NAkahara T. 1993. Development of androgenetic mouse embryos produced by in vitro fertilization of enucleated oocytes. Mol Reprod Dev 1993, 34(1): 43-46. https://doi.org/10.1002/mrd.1080340107
  17. Marcus GJ. 1990. Activation of cumulus-free mouse oocytes. Mol. Reprod. Dev. 26: 159-162. https://doi.org/10.1002/mrd.1080260210
  18. Miki H, Inoue K, Kohda T, Honda A, Ogonuki N, Yuzuriha M, Mise N, Matsui Y, Baba T, Abe K, Ishino F and Ogura A. 2005. Birth of mice produced by germ cell nuclear transfer. Genesis 41: 81-86. https://doi.org/10.1002/gene.20100
  19. Ogura A, Inoue K, Ogonuki N, Noguchi A, Takano K, Nagano R, Suzuki O, Lee J, Ishino F and Matsuda J. 2000. Production of male cloned mice from fresh, cultured, and cryopreserved immature sertoli cells. Biol. Reprod. 62: 1579-1584. https://doi.org/10.1095/biolreprod62.6.1579
  20. Ohgane J, Wakayama T, Kogo Y, Senda S, Hattori N, Tanaka S, Yanagimachi R and Shiota K. 2001. DNA methylation variation in cloned mice. Genesis 30: 45-50. https://doi.org/10.1002/gene.1031
  21. O'Neill GT, Rolfe LR and Kaufman MH. 1991. Developmental potential and chromosome constitution of strontium induced mouse parthenogenomes. Mol. Reprod. Dev. 30: 214-219. https://doi.org/10.1002/mrd.1080300308
  22. Onish A, Iwamoto M, Akita T, Mikawa S, Takeda K, Awata T, Hanada H and Perry ACF. 2000. Pig cloning by microinjection of fetal fibroblast nuclei. Science 289: 1188-1190. https://doi.org/10.1126/science.289.5482.1188
  23. Ono Y, Shimozawa N, Ito M and Kono T. 2001. Cloned mice from fetal fibroblast cells arrested at metaphase by a serial nuclear transfer. Biol. Reprod. 64: 44-50. https://doi.org/10.1095/biolreprod64.1.44
  24. Otaegui PJ, O'neill GT and Wilmut I. 1999. Parthenogenetic activation of mouse oocytes by exposure to strontium as a source of cytoplasts for nuclear transfer. Cloning 1: 111-117. https://doi.org/10.1089/15204559950019997
  25. Polejaeva IA, Chen SH, Vaught TD, Page RL, Mullins J, Ball S, Dai Y, Boone J, Walker S, Ayares DL, Colman A and Campbell KH. 2000. Cloned pigs produced by nuclear transfer from adult somatic cells. Nature 407: 86-90. https://doi.org/10.1038/35024082
  26. Ramirez-Solis R, Davis AC and Bradley A. 1993. Gene targeting in embryonic stem cells. Methods Enzymol. 225: 855-878. https://doi.org/10.1016/0076-6879(93)25054-6
  27. Rybouchkin A, Kato Y and Tsunoda Y. 2006. Role of histone acetylation in reprogramming of somatic nuclei following nuclear transfer. Biol. Reprod. 74: 1083-1089. https://doi.org/10.1095/biolreprod.105.047456
  28. Shin T, Kraemer D, Pryor J, Liu L, Rugila J, Howe L, Buck S, Murphy K, Lyons L and Weshusin MA. 2000. Cat cloned by nuclear transplantation. Nature 415: 859.
  29. Siracusa G, Whittingham DG and De Felici M. 1980. The effect of microtubule- and microfilament-disrupting drugs on preimplantation mouse embryos. J. Embryo Exp. Morphol. 60: 71-82.
  30. Snow MH. 1973 Tetraploid mouse embryos produced by cytochalasin B during cleavage. Nature 244: 513-515. https://doi.org/10.1038/244513a0
  31. Summers MC, Bhatnager PR, Lawitts JA and Biggers JD. 1995. Fertilization in vitro of mouse ova from inbred and outbred strains: complete preimplantation embryo development in glucose-supplemented KSOM. Biol. Reprod. 53: 431-437. https://doi.org/10.1095/biolreprod53.2.431
  32. Wakayama T, Perry AC, Zuccotti M, Johnsom KR and Yanagimachi R. 1998. Full-term development of mice from enucleated oocytes injected with cumulus cell nuclei. Nature 394: 369-374. https://doi.org/10.1038/28615
  33. Wakayama T, Rodriguez I, Perry AC, Yanagimachi R and Mombaerts P. 1999. Mice cloned from embryonic stem cells. Proc. Natl. Acad. Sci. USA 96: 14984-14989. https://doi.org/10.1073/pnas.96.26.14984
  34. Wakayama S, Suetsugu R, Thuan NV, Ohta H, Kishigami S and Wakayama T. 2007. Establishment of mouse embryonic stem cell lines from somatic cell nuclei by nuclear transfer into aged, fertilization-failure mouse oocytes. Current Biol. 17: 120-121. https://doi.org/10.1016/j.cub.2006.12.020
  35. Wakayama T and Yanagimachi R. 1999. Cloning of male mice from adult tail-tip cells. Nat. Genet. 22: 127-128. https://doi.org/10.1038/9632
  36. Wakayama T and Yanagimachi R. 2001. Effect of cytokinesis inhibitors, DMSO and the timing of oocyte activation on mouse cloning using cumulus cell nuclei. Reproduction 122: 49-60. https://doi.org/10.1530/rep.0.1220049
  37. Wells DN, Misica PM and Tervit HR. 1999. Production of cloned calves following nuclear transfer with cultured adult mural granulosa cells. Biol. Reprod. 60: 996-1005. https://doi.org/10.1095/biolreprod60.4.996
  38. Wilmut I, Schnieke AE, McWhir J, Kind AJ and Campbell KH. 1997. Viable offspring derived from fetal and adult mammalian cells. Nature 385: 810-813. https://doi.org/10.1038/385810a0
  39. Yang X, Smith SL, Tian XC, Lewin HA, Renard JP and Wakayama T. 2007. Nuclear reprogramming of cloned embryos and its implications for therapeutic cloning. Nat. Genet. 39: 295-302. https://doi.org/10.1038/ng1973
  40. Young LE, Sinclair KD and Wilmut I. 1998. Large offspring syndrome in cattle and sheep. Rev. Reprod. 3: 155-163. https://doi.org/10.1530/ror.0.0030155