가교결합 아피오스 전분의 이화학적 특성

Physicochemical Properties of Cross-linked Apios Starch

  • 박미혜 (경북대학교 식품영양학과) ;
  • 김미라 (경북대학교 식품영양학과 장수생활과학연구소)
  • Park, Mi Hye (Dept. of Food Science and Nutrition, Kyungpook National University) ;
  • Kim, Meera (Dept. of Food Science and Nutrition, Center for Beautiful Aging, Kyungpook National University)
  • 투고 : 2014.05.08
  • 심사 : 2014.06.30
  • 발행 : 2014.06.30

초록

본 연구에서는 아피오스로부터 전분을 추출하여 epichlorohydrin으로 가교결합을 시킨 후 가교결합 아피오스 전분의 이화학적 특성을 분석하였다. 가교결합 아피오스 전분은 치환도가 증가함에 따라 몰가교화도가 0.1225에서 3.5124로 비례적으로 증가하였으며, FT-IR을 측정한 결과, CLAP-2%는 에테르 결합으로 인하여 $1,646cm^{-1}$$2,930cm^{-1}$에서 피크의 강도가 강해져 가교결합 반응이 이루어졌음을 확인할 수 있었다. 천연 아피오스 전분의 X-선 회절도는 $2{\theta}$=15.1, 17.1, 17.9, $23.2^{\circ}$에서 피크가 나타나는 전형적인 A형 전분 형태를 나타내었으며, 가교결합 아피오스 전분은 가교화에 의해 회절도와 상대결정화도에 큰 변화를 보이지 않아 무결정 영역에서 가교결합 반응이 일어난 것으로 추정되었다. 아피오스 전분 입자를 SEM으로 관찰한 결과, 전분 입자는 구형 또는 타원형 모양을 나타내었으며, 가교결합 아피오스 전분은 천연 아피오스 전분과 비교하였을 때 전체적인 형태에는 변화가 없는 것이 관찰되었다. 색도는 가교결합이 진행되면서 명도와 청색도가 변화하였으며, 청가는 천연 아피오스 전분과 가교결합 아피오스 전분 사이에 유의적인 차이가 나타나지 않았다.

Apios (Apios americana Medikus) belongs to Leguminosae and is called 'American groundnut', 'Potato bean', and 'wild bean'. Apios is native to the Northern United States but is not widely distributed in Korea. In this study, cross-linked apios starch was prepared by reaction with epichlorohydrin, followed by characterization. FT-IR spectroscopy confirmed the degree of cross-linking of apios starch. X-ray diffraction patterns of native apios showed typical 'A' type as peaks at 15.1, 17.1, 17.9 and $23.2^{\circ}$, and cross-linking did not affect relative crystallinity and X-ray diffraction patterns of the starch. Scanning electron micrographs showed that apios starch granules were smooth with a globular shape, and there was little damage to starch granules after cross-linking. The lightness value of cross-linked apios starch was lower than that of native apios starch, whereas the redness value was not significantly different between cross-linked apios starch and native apios starch. Blue value showed that cross-linking of starch did not affect the iodine reaction of starch.

키워드

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