DOI QR코드

DOI QR Code

아토피 피부염 외치치료제 처방 개발을 위한 실험적 연구

An experimental study for the development of prescription on atopic dermatitis

  • 김건우 (대전대학교 한의과대학 병리학교실) ;
  • 박지원 (대전대학교 한의과대학 병리학교실) ;
  • 심부용 (대전대학교 한의과대학 병리학교실) ;
  • 김동희 (대전대학교 한의과대학 병리학교실)
  • Kim, Gun Woo (Department of Pathology, College of Oriental Medicine, Daejeon University) ;
  • Bak, Ji Won (Department of Pathology, College of Oriental Medicine, Daejeon University) ;
  • Sim, Boo-Yong (Department of Pathology, College of Oriental Medicine, Daejeon University) ;
  • Kim, Dong Hee (Department of Pathology, College of Oriental Medicine, Daejeon University)
  • 투고 : 2014.06.30
  • 심사 : 2014.07.16
  • 발행 : 2014.07.30

초록

Objectives : Atotang was composed of 10 kinds of traditional medicinal herb. This research was performed to examine biological effects of Atotang for the development of prescription on atopic dermatitis. Methods : Atotang was extracted with 80% EtOH. Free radical scavenging assay has tested for anti-oxidative activity as well as the contents of total polyphenol. We observed the production of ROS, nitric oxide(NO) and the inflammatory cytokines such as interleukin-1beta(IL-${\beta}$), IL-6, tumor necrosis factor-alpha(TNF-${\alpha}$), Prostaglandin E2($PGE_2$) in Raw 264.7 cells stimulated by LPS. We used Disc diffusion method to investigate antibacterial activity on Candida albicans, Staphylococcus aureus and Staphylococcus epidermis. Result : Content of total phenolic compound of Atotang was 36.3 mg/g ext. DPPH and ABTS scavenging activities were 77% and 46% at 200 ug/ml respectively, showing dose-dependent increase. The amounts of ROS and NO in RAW 264.7 cells were decreased by 30% and 19% at 200 ug/ml, respectively, showing dose-dependent decrease. The prodcution of IL-1beta, IL-6 and TNF-alpha in RAW 264.7 cells were decreased dose-dependently by 81%, 67%, and 20% at 200 ug/ml, respectively. Atotang was reduced LPS-stimulated production of $PGE_2$ by 33%. Atotang on C. albicans, S. aureus and S. epidermis was selected by a disc diffusion method and inhibition effect of the Atotang on the growth of S. epidermis was the greatest. Conclusion : The results indicated that Atotang showed biological activities showing anti-oxidant, anti-inflammatory and antibacterial effects. Based on these results, it is concluded that Atotang can be applied to the prescription on atopic dermatitis.

키워드

참고문헌

  1. Yang GS. A study on anatibiotic effect of aroma oils on the growth of Staphylococcus aureus existing in the skin of the atopic dermatitis. Chonnam National University. 2007.
  2. Lee GH. How aroma oil affects animal model with atopic dermatitis by DNCB on anti-inflammatory and immune. Daejeon University. 2010.
  3. Cho SE. Anti-oxidative, anti-inflammatory and immunomodulatory effects of chamaecyparis obtusa leaves extracts for atopic dermatitis. Konkuk University. 2011.
  4. Kim JJ, Yang SW, Son NW, Ahn KS. Anti-inflammatory action by gamisangryosamul-tang and the effect of ziyang-go on atopic dermatitis-like lesion and pruritus in NC/Nga mice. Korean J Orient Physiol Pathol. 2003 ; 17(2) : 428-35.
  5. Kwon HJ, Kim YJ, Jang SH, Bae BK, Youn HY, Lee HW. Stimulatory effect of staphylococcal protein A on inflammatory response in human HaCaT keratinocytes. Korean J Microbiol. 2011 ; 47(4) : 348-55.
  6. Kwon HY. The anti-atopic effects of flavonol content from Ginkgo biloba leaves on NC/Nga murine model with mast cell and DNCB-induced atopy. Daejeon University. 2012.
  7. Park DB, Han JK, Kim YH. Effect of Yanghyeuljeseuptang on immunological factors in spleen and draining lymph node of atopic dermatitis induced NC/Nga mouse by dinitrochlorobenzene. Res inst Korean Med. 2007 ; 16(2) : 251-65.
  8. Yu JS. The effects of lonicerae flos, forsythiae fluctus and hwangryunhaedok decoction pharmacopuncture on atopic dermatitis in NC/Nga mice. Woosuk University. 2012.
  9. Sim BY, Ji JG, Lee WY, Kim SJ, Kim HY, Lee JY, Kim DH. Experimental research of baechsunpijibujabokhap-banng on atopic dermatitis treatment. Res inst Korean Med. 2014 ; 22(2) : 67-79.
  10. Bang CK, Choi JJ, Eom DM, Kim DH. The effects of BGG on various immunological factors related to pathogenesis of allergic dermatitis in NC/Nga mice induced by der-f. Res inst Korean Med. 2007 ; 16(2) : 147-69.
  11. Jung JH. Anti-inflammatory effects of herbal mixture(Rubus coreanus, Rehmanniae Radix, Houttuynia cordata, Betulae cortex) on acute atopic dermatitis mice. Kyungwon University. 2010.
  12. Ko HY, Kim DH. Study on combination of external gosamgamibang and internal chenggihaedok-san for the treatment of atopic dermatitis. Korea J Orient Physiol Pathol. 2009 ; 23(6) : 1282-91.
  13. Kim TH. Alleviation of atopic dermatitis symptoms by extracts of camellia sinensis. Gyeongsang National University. 2013.
  14. Lee IH, Lee SH, Lee IS, Park YK, Chung DK, Choue RW. Effects of probiotic extracts of kimchi on immune function in NC/Nga mice. Korean J Food Sci Technol. 2008 ; 40(1) : 82-7.
  15. Jo YR, Kang SM. Lactococcus lactis culture methods for the enhanced depression of inducers in atopic diseases. Korean J Microbiol Biotechnol. 2012 ; 40(4) : 310-8. https://doi.org/10.4014/kjmb.1205.05025
  16. Kim JM, Oh HC, Song SP, Kim NK, Hwang CY. Study on the anti-microbacterial activity, anti-inflammatory and anti-allergic effects of several herb-extract. Korean J Oreint Physiol Pathol. 2006 ; 20(1) : 103-14.
  17. Diagnosis and treatment of atopic dermatitis. GoonJa publisher. NamDongHo. 2010. Available from:URL:http://dr_allergy.blog.me/30106658996
  18. Yoo HS. A study on the immune modulation of chunghwatang(CHT) in Atopic dermatitis antimal models. Daejeon University. 2012.
  19. Niwa Y, Sumi H, Kawahira K, Terashima T, Nakamura T, Akamatsu H. Protein oxidative damage in the stratum corneum: evidence for a link between environmental oxidants and the changing prevalence and nature of atopic dermatitis in Japan. Br J Dermatol. 2003 ; 149(2) : 248-58. https://doi.org/10.1046/j.1365-2133.2003.05417.x
  20. Omata N, Tsukahara H, Ito S, Ohshima Y, Yasutomi M, Yamada A, Jiang M, Hiraoka M, Nambu M, Deguchi Y, Mayumi M. Life Sci. 2001 ; 69(2) : 223-8. https://doi.org/10.1016/S0024-3205(01)01124-9
  21. Taniuchi S, Kojima T, Hara K, Yamamoto A, Sasai M, Takahashi H, Kobayashi Y. Increased serum nitrate levels in infants with stopic dermatitis. Allergy. 2001 ; 56 : 693-5. https://doi.org/10.1034/j.1398-9995.2001.00131.x
  22. Ryu SY, Yang KS. Antioxidative activities of solvent fractions from sophora flavescens. Journal of Pharmaceuticel Sciences Sookmyung Women's University. 2006 ; 23 : 18-21.
  23. Song DA. Anti-stopic effect in Nc/Nga mouse by oxymatrine, matrine, trifolirhizin, kurarinone of sophora flavescens solander ex aiton. Konkuk University. 2010.
  24. Lee DJ, Jeon IH, Kim HS, Cho IY, Jang SI. Antioxidative and anti-inflammatory effect of ethanol extract from duchesnea chrysantha. Korean J Orient Physiol Pathol. 2012 ; 26(1) : 59-66.
  25. Jo GH. Effect of paeoniae radix alba or dictamini radicis cortex application on atopic dermatitis like immune alterations in mice. Catholic University of Daegu. 2012.
  26. Na SH, Ko SG, Shin YC. Effects of kochiae fructus extracts on the expression of angiogenesis and inflammation related proteins. Korean J Orient Physiol Pathol. 2006 ; 20(3) : 557-62.
  27. Seo WS, Oh HN, Park WJ, Um SY, Lee DW, Kang SM. Study on the anti-inflammatory effect and mechanism of prunus mume extract regarding NF-$\kappa{B}$. Korean Society for Biotechnology and Bioengineering Journal. 2014 ; 29(1) : 50-57. https://doi.org/10.7841/ksbbj.2014.29.1.50
  28. Lee SY, Lee YJ, Park WS. Anti-inflammatory effects of fermented houttuyniae herba water extract on LPS-induced mouse macrophage. Kor J Herbology. 2010 ; 25(3) ; 27-34.
  29. Min SH. A comparative study on the anti-oxidant effect of adenophorae radix, codonopsis lanceolatae radix and glehniae radix cum rhizoma. Kyungwon University. 2006.
  30. Lee KI, Kim SM. Antioxidative and antimicrobial activities of Eriobotrya japonica Lindl. leaf extracts. J Korean Soc Food Sci Nutr. 2009 ; 38(1) : 267-73. https://doi.org/10.3746/jkfn.2009.38.3.267
  31. Jang TO, Yoo YH, Hwang YC, Kim HK, Woo HC. Total polyphenol content and antioxidative activities of mistletoe (Viscum album) extracts by supercritical carbon dioxide. J Korean Soc Food Sci Nutr. 2010 ; 39(1) : 20-4. https://doi.org/10.3746/jkfn.2010.39.1.020
  32. Hong SC, Jun JA, Kim DH. Effect of Betulae Cortex against oxidative stress. Korean J Orient Physiol Pathol. 2013 ; 27 : 391-9.
  33. Hong SM. Effect of Kyejakjimotangkami on osteoarthritis. Daejeon University. 2013.