Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Antioxidant Activities of Extracts from Leaves and Stems of Achyranthes japonica

쇠무릎 잎과 줄기 추출물의 항산화 활성 연구

  • Seo, Soo Jung (Dept. of Herbal Biotechnology, Daegu Haany University) ;
  • Kim, Nam Woo (Dept. of Herbal Biotechnology, Daegu Haany University)
  • 서수정 (대구한의대학교 한약자원학과) ;
  • 김남우 (대구한의대학교 한약자원학과)
  • Received : 2014.03.03
  • Accepted : 2014.05.26
  • Published : 2014.07.31
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Abstract

This study was conducted to investigate the physiological characteristics of water and ethanol extracts from leaves and stems of Achyranthes japonica. The highest contents of total polyphenol and flavonoid compounds were 58.27 and 42.22 mg/g in water extract from leaves, respectively. The protein content was the highest at 16.42 mg/g in water extract from leaves. Ethanol extract from stems showed the highest content of reducing sugars at 11.35 mg/g. In the measurement of electron donating ability (EDA), ethanol extract from stems showed the highest EDA at 93.41% at a concentration of 1.0 mg/mL. Superoxide dismutase-like activity of ethanol extract from leaves was the highest at 8.13% at a concentration of 1.0 mg/mL. In the analysis of nitrate scavenging activity, water extract from leaves showed the highest activity at 94.90% at pH 1.2, and the activity increased as concentration increased and pH decreased. In the measurement of xanthine oxidase inhibition, ethanol extract from stems showed the highest inhibitory activity at 66.67% at a concentration of 1.0 mg/mL. Especially, nitrate scavenging activities of water extract from leaves were the highest under all pH conditions. These results verify that extracts from leaves of A. japonica have strong antioxidant activity and can be used as an effective antioxidant source for nutraceutical foods, medicines, and cosmetic stuffs.

한방에서 건조된 뿌리를 '우슬'이라는 약재로 이용되는 쇠무릎의 잎과 줄기를 물과 에탄올을 용매로 추출하여 유용성분과 항산화 활성을 측정하였다. 폴리페놀과 플라보노이드 화합물의 총 함량을 측정한 결과 쇠무릎 잎의 물 추출물인 LWE에서 각각 58.27 mg/g과 42.22 mg/g으로 가장 많은 함량을 나타내었고, 단백질 함량에서도 LWE에서 16.42 mg/g으로 가장 많이 함유된 것으로 분석되었다. 환원당 함량은 줄기의 에탄올 추출물인 SEE에서 11.35 mg/g으로 가장 많았으며 전자공여능은 1.0 mg/mL의 농도에서 SEE가 93.41%로 가장 우수한 전자공여 효과를 보였다. SOD 유사활성능은 1.0 mg/mL의 농도에서 모든 추출물이 10% 미만의 낮은 활성을 나타내었다. 아질산염 소거능은 pH 1.2의 조건의 1.0 mg/mL의 농도에서 LWE가 94.90%의 가장 높은 아질산염 소거능을 나타내었으며, pH 3.0에서도 LWE가 45.20%로 가장 높은 소거 활성을 나타내었다. LWE는 1.0 mg/mL 66.67%로 가장 높은 XO 저해 효과를 나타내었으며, LEE와 SWE도 각각 31.17%와 32.06%의 XO 저해율을 나타내었다. 이상의 결과 쇠무릎 잎은 다량의 폴리페놀 및 플라보노이드 화합물을 함유하였으며 전자공여능과 SOD 유사활성능, 아질산염 소거 효과도 줄기보다 우수하며 식용 및 약용식물로 이용되는 여러 식물보다 우수하여 쇠무릎 잎을 식용뿐만 아니라 기능성 식품의 첨가제나 천연 항산화제로서 활용될 수 있을 것이라 판단된다.

Keywords

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