Journal of the Korean Society for Precision Engineering (한국정밀공학회지)

Korean Society for Precision Engineering (한국정밀공학회)
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Fabrication of HepG2 Cell Laden Collagen Microspheres using Inkjet Printing

잉크젯 프린팅을 이용한 HepG2 세포 담지 콜라겐 마이크로스피어 제작

  • Choi, Jin Ho (School of Mechanical Engineering, Kyungpook National University) ;
  • Kim, Young Ho (Medical Device Development Center, Daegu-Gyeongbuk Medical Innovation Foundation) ;
  • Jacot-Descombes, Loic (Ecole Polytechnique Federale de Lausanne (EPFL)) ;
  • Brugger, Jurgen (Ecole Polytechnique Federale de Lausanne (EPFL)) ;
  • Kim, Gyu Man (School of Mechanical Engineering, Kyungpook National University)
  • Received : 2014.02.03
  • Accepted : 2014.06.25
  • Published : 2014.08.01
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Abstract

In this study, drop-on-demand system using piezo-elecrtric inkjet printers was employed for preparation of collagen microspheres, and its application was made to the HepG2 cell-laden microsphere preparation. The collagen microspheres were injected into beaker filled with mineral oil and incubated in a water bath at $37^{\circ}C$ for 45 minutes to induce gelation of the collagen microsphere. The size of collagen microsphere was $100{\mu}m$ in diameter and $80{\mu}m$ in height showing spherical shape. HepG2 cells were encapsulated in the collagen microsphere. The cell-laden microspheres were inspected by the microscopic images. The encapsulation of cells may be beneficial for applications ranging from tissue engineering to cell-based diagnostic assays.

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References

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