한국임상수의학회지 (Journal of Veterinary Clinics)

  • 제31권3호
  • /
  • Pages.180-193
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  • 2014
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  • 1598-298X(pISSN)
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  • 2384-0749(eISSN)
한국임상수의학회 (The Korean Society of Veterinary Clinics)
권호 표지

개에서 DNCB에 의해 유발된 알레르기성 접촉피부염에 대한 아로마 오일 합제의 항염증 효과

Anti-inflammatory Effects of Aroma Oil Complex on DNCB-Induced Allergic Contact Dermatitis in Dogs

  • 오동규 (경북대학교 수의과대학 수의피부과학실험실) ;
  • 오태호 (경북대학교 수의과대학 수의피부과학실험실)
  • Oh, Dong-Kyu (Laboratory of Veterinary Dermatology, College of Veterinary Medicine, Kyungpook National University) ;
  • Oh, Tae-Ho (Laboratory of Veterinary Dermatology, College of Veterinary Medicine, Kyungpook National University)
  • 심사 : 2014.06.19
  • 발행 : 2014.06.30
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초록

알러지성 접촉피부염은 T세포와 대식세포가 관여하는 세포매개성 면역반응으로 항원에 노출된 뒤 수 일 후에 증상이 발현되는 지연형 반응으로 특이 T세포의 활성을 통해 다양한 cytokine이 분비되어 항원 유입부위로 염증세포의 유주로 염증이 발생한다. 본 연구에서는 DNCB로 유발한 알러지성 접촉피부염에 대한 아로마 오일 합제의 항염효과를 평가하였다. 아로마 오일 합제는 8일간 적용하였고 실험기간 동안 2일 간격으로 피부 생물리학적 지표를 측정한 후 피부생검을 통해 피부조직을 평가하였다. DNCB 적용 후 피부 생물리학적 지표인 표피경유수분손실, 피부 수화도, 피부 두께 그리고 홍반지수가 증가하였다 (p < 0.05). 피부조직 평가에서 염증세포 침윤과 부종성 변화에 의한 상피두께 증가 및 진피 결합조직의 감소가 특징적으로 나타났다. 또한 진피내 pro-inflammatory cytokine인 TNF-${\alpha}$와 IFN-${\gamma}$ 면역반응세포수 및 표피내 apoptotic change의 지표인 caspase-3와 PARP 면역반응세포의 수가 유의적으로 증가 하였다 (p<0.01). 그러나 아로마 오일 합제 적용후에는 피부 생물리학적 지표 (p<0.05) 및 피부조직의 병리적 변화는 (p<0.01) 기저상태로 회복되었다. 따라서 본 연구를 통해 개에서 DNCB에 의한 알레르기성 접촉피부염 유발 및 아로마 오일 합제의 우수한 항염증 효과를 확인하였고 아로마 오일 합제는 향후 피부염에 대한 치료제로 사용할 수 있을 것으로 기대된다.

Allergic contact dermatitis (ACD) is an inflammatory skin disease and regarded as a prototype of T-cell mediated delayed-type hypersensitivity reactions. Aroma Oil Complex (AOC) is composed of lavender true oil, chamomile roman oil and tea tree oil. This study was performed to assess the effects of AOC in a canine model of ACD. ACD was induced on the back of dogs induced by sensitization and repeated application by 2,4-dinitro-1-chlorobenzene (DNCB). Topical treatment of AOC was applied once a day for 8 days and skin biophysical parameters including transepidermal water loss (TEWL), skin hydration, skin thickness and erythema index, were measured every two days during experimental periods. Histopathology and immunohistochemistry were performed to evaluate the anti-inflammatory effect. In skin biophysical parameters, TEWL, skin hydration, skin thickness and erythema index were significantly increased, with a maximum increase appeared on day 2 (p<0.05). After the completion of AOC treatment, skin biophysical parameters were significantly reached those of baseline in a time-dependent manner (p<0.05). In histopathology, marked increases of epidermal thicknesses were induced after DNCB challenge with numerous inflammatory cell infiltrations and edematous changes, decreases of connective tissue occupied regions in dermis. In addition, marked increases of cytokine - tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$) and interferon-${\gamma}$ (IFN-${\gamma}$)-immunoreactivities in the dermis and of apoptotic markers - caspase-3 and PARP-immunoreactivities in the epidermis were observed in DNCB control as compared with intact control, respectively (p<0.01). The decrease of infiltrated inflammatory cells and related decreases of pro-inflammatory cytokine immunoreactivities were observed in AOC treated skin (p<0.01). Based on these findings, AOC may have anti-inflammatory and alleviatory effects in the allergic contact dermatitis.

키워드

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