The Korean Journal of Pain

  • 제27권3호
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  • Pages.229-238
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  • 2014
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  • 2005-9159(pISSN)
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  • 2093-0569(eISSN)
대한통증학회 (The Korean Pain Society)
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Bupivacaine-induced Vasodilation Is Mediated by Decreased Calcium Sensitization in Isolated Endothelium-denuded Rat Aortas Precontracted with Phenylephrine

  • Ok, Seong Ho (Department of Anesthesiology and Pain Medicine, Institute of Health Sciences, Gyeongsang National University School of Medicine, Gyeongsang National University Hospital) ;
  • Bae, Sung Il (Department of Anesthesiology, Samsung Changwon Hospital, Sungkyunkwan University School of Medicine) ;
  • Kwon, Seong Chun (Department of Physiology, Kwandong University College of Medicine) ;
  • Park, Jung Chul (Department of Anesthesiology and Pain Medicine, Gyeongsang National University Hospital) ;
  • Kim, Woo Chan (Department of Anesthesiology and Pain Medicine, Gyeongsang National University Hospital) ;
  • Park, Kyeong Eon (Department of Anesthesiology and Pain Medicine, Gyeongsang National University Hospital) ;
  • Shin, Il Woo (Department of Anesthesiology and Pain Medicine, Institute of Health Sciences, Gyeongsang National University School of Medicine, Gyeongsang National University Hospital) ;
  • Lee, Heon Keun (Department of Anesthesiology and Pain Medicine, Institute of Health Sciences, Gyeongsang National University School of Medicine, Gyeongsang National University Hospital) ;
  • Chung, Young Kyun (Department of Anesthesiology and Pain Medicine, Institute of Health Sciences, Gyeongsang National University School of Medicine, Gyeongsang National University Hospital) ;
  • Choi, Mun Jeoung (Department of Oral and Maxillofacial Surgery, Gyeongsang National University Hospital) ;
  • Sohn, Ju Tae (Department of Anesthesiology and Pain Medicine, Institute of Health Sciences, Gyeongsang National University School of Medicine, Gyeongsang National University Hospital)
  • 투고 : 2014.03.10
  • 심사 : 2014.03.17
  • 발행 : 2014.07.01
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초록

Background: A toxic dose of bupivacaine produces vasodilation in isolated aortas. The goal of this in vitro study was to investigate the cellular mechanism associated with bupivacaine-induced vasodilation in isolated endothelium-denuded rat aortas precontracted with phenylephrine. Methods: Isolated endothelium-denuded rat aortas were suspended for isometric tension recordings. The effects of nifedipine, verapamil, iberiotoxin, 4-aminopyridine, barium chloride, and glibenclamide on bupivacaine concentration-response curves were assessed in endothelium-denuded aortas precontracted with phenylephrine. The effect of phenylephrine and KCl used for precontraction on bupivacaine-induced concentration-response curves was assessed. The effects of verapamil on phenylephrine concentration-response curves were assessed. The effects of bupivacaine on the intracellular calcium concentration ($[Ca^{2+}]_i$) and tension in aortas precontracted with phenylephrine were measured simultaneously with the acetoxymethyl ester of a fura-2-loaded aortic strip. Results: Pretreatment with potassium channel inhibitors had no effect on bupivacaine-induced relaxation in the endothelium-denuded aortas precontracted with phenylephrine, whereas verapamil or nifedipine attenuated bupivacaine-induced relaxation. The magnitude of the bupivacaine-induced relaxation was enhanced in the 100mM KCl-induced precontracted aortas compared with the phenylephrine-induced precontracted aortas. Verapamil attenuated the phenylephrine-induced contraction. The magnitude of the bupivacaine-induced relaxation was higher than that of the bupivacaine-induced $[Ca^{2+}]_i$ decrease in the aortas precontracted with phenylephrine. Conclusions: Taken together, these results suggest that toxic-dose bupivacaine-induced vasodilation appears to be mediated by decreased calcium sensitization in endothelium-denuded aortas precontracted with phenylephrine. In addition, potassium channel inhibitors had no effect on bupivacaine-induced relaxation. Toxic-dose bupivacaine-induced vasodilation may be partially associated with the inhibitory effect of voltage-operated calcium channels.

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참고문헌

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  3. A Lipid Emulsion Reverses Toxic-Dose Bupivacaine-Induced Vasodilation during Tyrosine Phosphorylation-Evoked Contraction in Isolated Rat Aortae vol.18, pp.2, 2017, https://doi.org/10.3390/ijms18020394
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  6. The Mechanisms Underlying Lipid Resuscitation Therapy vol.43, pp.2, 2018, https://doi.org/10.1097/AAP.0000000000000719
  7. Lipid Emulsion Inhibits Vasodilation Induced by a Toxic Dose of Bupivacaine via Attenuated Dephosphorylation of Myosin Phosphatase Target Subunit 1 in Isolated Rat Aorta vol.12, pp.12, 2015, https://doi.org/10.7150/ijms.13299
  8. Dexmedetomidine Inhibits Phenylephrine-induced Contractions via Alpha-1 Adrenoceptor Blockade and Nitric Oxide Release in Isolated Rat Aortae vol.14, pp.2, 2014, https://doi.org/10.7150/ijms.17456