Korean Journal of Veterinary Service (한국동물위생학회지)

The Korean Society of Veterinary Service (한국동물위생학회)
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Origin and effective ingredient standards of honeybee venom as natural antibiotic ingredients

천연항생제로서 봉독의 기원 및 지표성분 설정

  • Han, SangMi (National Institute of Agricultural Science and Technology) ;
  • Kim, JungMin (National Institute of Agricultural Science and Technology) ;
  • Han, SangHoon (Animal and Plant Quarantine Agency) ;
  • Yeo, JooHong (National Institute of Agricultural Science and Technology) ;
  • Hong, InPyo (National Institute of Agricultural Science and Technology) ;
  • Woo, SoonOk (National Institute of Agricultural Science and Technology) ;
  • Lee, KwangGill (National Institute of Agricultural Science and Technology) ;
  • Kweon, HaeYong (National Institute of Agricultural Science and Technology)
  • 한상미 (농촌진흥청 국립농업과학원) ;
  • 김정민 (농촌진흥청 국립농업과학원) ;
  • 한상훈 (농림축산검역본부) ;
  • 여주홍 (농촌진흥청 국립농업과학원) ;
  • 홍인표 (농촌진흥청 국립농업과학원) ;
  • 우순옥 (농촌진흥청 국립농업과학원) ;
  • 이광길 (농촌진흥청 국립농업과학원) ;
  • 권해용 (농촌진흥청 국립농업과학원)
  • Received : 2014.05.14
  • Accepted : 2014.06.27
  • Published : 2014.06.30
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Abstract

This research was performed in order to investigate the origin, standard compound, and structural and physical properties of honeybee venom which used as natural antibiotic ingredients to animal. We compared the nucleotide sequence of mitochondrial cytochrome c oxidase subunit 1 gene (COI) of honeybees were collected from Gangwon, Gyeonggi, Chungnam, Gyeongbuk, Gyeongnam province and Suwon. As major constituent of honeybee venom, melittin was assayed by liquid chromatography. X-ray, differential scanning calorimetry (DSC) and fourier transform infrared spectroscopy (FT-IR) were utilized to examine the structural and physical properties of honeybee venom. Based on the 627bp sequence of COI, Apis mellifera ligustica was determinated honeybees collected from all six regions. Melittin content varied from 50.7 to 68.6 and averaged 59.8%. According to XRD analysis, honeybee venom showed regular crystal structure peaks at $2{\Theta}=8.5^{\circ}$ and $21.5^{\circ}$. DSC showed that the maximum degration temperature of powder was around $230^{\circ}C$. Through FT-IR analysis, we could identify cross-linking by the presence of peptide peak at 1,500~1,600 $cm^{-1}$. In conclusion, the origin of honeybee venom was Apis mellifera ligustica and effective ingredient standards was melittin content varied from 50.7 to 68.6 as natural antibiotic ingredients.

Keywords

References

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