The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
권호 표지
DOI QR코드

DOI QR Code

Anti-inflammatory effect of Baecksunpijibujabokhap-bang in Atopic dermatitis model mice

아토피 피부염을 유발한 마우스에서 백선피지부자복합방의 항염증 효과

  • Sim, Boo-Yong (Dept. of Pathology, College of Oriental Medicine, Daejeon University) ;
  • Kim, Sung-Hwan (Dept. of Food & Nutrition, Joongbu University) ;
  • Kim, Dong-Hee (Dept. of Pathology, College of Oriental Medicine, Daejeon University)
  • 심부용 (대전대학교 한의과대학 병리학교실,) ;
  • 김성환 (중부대학교 식품영양학과) ;
  • 김동희 (대전대학교 한의과대학 병리학교실,)
  • Received : 2014.04.25
  • Accepted : 2014.05.14
  • Published : 2014.05.30
Download PDF
⟨ Previous Next ⟩

Abstract

Objectives : In order to investigate the efficacy of BJBB on atopic dermatitis, various anti-inflammatory factors were studied. Methods : In-vitro, inflammatory mediators, such as MTT and nitric oxide were detected after the addition of LPS with or without BJBB in Raw 264.7 cells. In-vivo, in order to verify the effectiveness of BJBB in atopic dermatitis animal model, its role in inflammation factors and histological changes were observed in NC/Nga mice. Results : BJBB showed cell viability of 100% or higher in all concentration in Raw 264.7 cells. BJBB inhibited LPS-induced productions of inflammatory mediators nitric oxide in RAW 264.7cells. BJBB treated group showed significant decrease in the expression of IL-1b, IL-6 and TNF-a by 40%, 80% and 44% respectively. Also the group showed decrease in the transcription of IL-1b, IL-6 and TNF-a mRNA in spleen by 41%, 93% and 39% respectively. BJBB treated group showed significant decrease in WBC, neutrophil, lympocyte and monocytes immune cell ratio in blood by 54%, 63%, 57% and 86% respectively. BJBB treated group showed decrease in the expression of IgG by 39% respectively. Also, infiltration of adipocytes into skin was suppressed and the thickness of epidermis and dermis were relatively decreased in the BJBB treated group. Conclusion : BJBB has an anti-inflammatory effects in NC/Nga mouse. Thus, these results suggested a beneficial effect of BJBB in treatment with Atopic dermatitis and inflammatory.

Keywords

References

  1. Galli SJ, Gordon JR, Wershil BK. Mast cell cytokines in allergy and inflammation. Agents and Actions Supplements. 1993 ; 43 : 209-20.
  2. Koblenzer CS. Itching and the Atopic Skin. J Allergy Clin Immunol. 1999 ; 104 : 109-13. https://doi.org/10.1016/S0091-6749(99)70052-7
  3. Mihara K, Kuratani K, Matusui T, Yokota K. Vital Role of the Itch-scratch Response in Development of Spontaneous Dermatitis in NC/Nga Mice. Br J Dermatol. 2004 ; 151(2) : 335-45. https://doi.org/10.1111/j.1365-2133.2004.06036.x
  4. Park MC. A literature study about comparison of Eastern-Western medicine on the Atopic dermatitis. Wonkwang Hanny University. 2001.
  5. Kang HS. Comparative Study of Cheongyeoltang and Cheongyeoltangbalhyobang on Atopic Dermatitis. Daejeon Hanny University. 2010.
  6. Park JS, Sin TY, Kim DK, Lee JH. The Effects of Dictamni Radicis Cortex on the iNOS Expression and Proinflammatory Cytokines Production. Kor J Pharmacogn. 2011 ; 42(4) : 348-53.
  7. Lee YS. The rapeutic effects of Dictamni Radicis Cortex extracts on atopic dermatitis-like skin lesions of NC/Nga mouce induced by DNCB. Daejeon Hanny University. 2014.
  8. Na SH. K.F protein expression of angiogenic and inflammatory mediators impact. Kyunghee Hanny University. 2006.
  9. Margarete N, Thomas W. Innate immunity, allergy and atopic dermatitis. Current Opinion in Allergy and Clinical Immunology. 2010 ; 10(5) : 463-8. https://doi.org/10.1097/ACI.0b013e32833e3163
  10. Hong JH. A research Western medical science, The Korean traditional medicine, Local clinic and Naturopathy about Atopy. The Korean Society For Eco Early Childhood Education. 2008 ; 7(1) : 65-81.
  11. Bang SH, Choi YW, Ham JH, Hwan GG. The Clinical Effect of Topical DPCP Therapy in the Treatment of Atopic Dermatitis in Children and Adolescents. Korean J Dermatol. 2004 ; 42(1) : 29-36.
  12. Jung SY, Seo HS. The Effects of Samhwangseje on Anti-Inflammation in Raw 264.7 cell. J Korean Orient Med Ophthalmol Otolaryngol Dermatol. 2009 ; 22(1) : 108-19.
  13. Tak MJ, Tak MR, Kang KH, Ko WS, Yoon HJ. The Inhibitory Effects of Yang Geouk San Hwa-Tang on LPS-stimulated inflammation in RAW264.7 macrophage cells. J Korean Orient Med Ophthalmol Otolaryngol Dermatol. 2010 ; 23(1) : 118-34.
  14. Ryu IH, Cho HB, Kim SB, Seo YJ, Choi CM. The inhibitory effect of Picrasmae Lignum on inflammatory responses. J Orient Obstet Gynecol. 2011 ; 24(1) : 1-14.
  15. Kim CS, Yoon JH, Park DH. The Effects of Exercise at Anaerobic Threshold Intensity on Production of NO, IL-1 beta and IFN-gamma in Young Males. J Korean Soc Living Environ Syst. 2012 ; 19(3) : 381-91.
  16. Lee GS, Pena ID, Choi JY, Yoon SY, Choi JH, Kang TJ, Oh SK, Cheong JH. Effect of SPZZC, a composotion of herb extracts, on atopic dermatitis in BALB/c and NC/Nga mouse. Yakhak Hoeji. 2008 ; 52(3) : 232-9.
  17. Ha JC, Kim HD, Joo JW, Kim JO. Ultrastructural Localization of Antigenic Sites on Vibrio vulnificus and BALB/c Spleen Cells Applying the Protein A-Gold Techique. J Korean Soc Microbiol. 1989 ; 24(4) : 391-7.
  18. Chung HT, Ha TY, Cung DK. Histological changes of mouse spleen and lymph node by cyclophosphamide. J Korean Soc Microbiol. 1978 ; 10 : 55-62.
  19. Lee BG, Chu MK, Chung HK, Kim JD. Effect of Adaptagen-$\alpha$ on the Mouse Peritoneal Macrophages and Spleen Cells in Vivo. J Korean Soc Microbiol. 1978 : 29(5) : 507-15.
  20. Peter P. The immune system. 2nd rev. ed. U.S.A : Garland Science. 2009 : 30-1.
  21. Kawakami T, Galli SJ. Regulation of mast-cell and basophil function and survival by IgE. Nat Rev Immunol. 2002 ; 2 : 773-86. https://doi.org/10.1038/nri914
  22. Simpson EL. Atopic dermatitis: a review of topical treatment options. Curr Med Res Opin. 2010 ; 26(3) : 633-40. https://doi.org/10.1185/03007990903512156
  23. Nagata N, Oshida T, Yoshida NL, Yuyama N, Sugita Y, Tsujimoto G, Katsunuma T, Akasawa A, Saito H. Analysis of highly expressed genes in monocytes from atopic dermatitis patients. Int Arch Allergy Immunol. 2003 ; 132 : 156-67. https://doi.org/10.1159/000073717
  24. Sim BY, Ji JG, Lee WY, Kim SJ, Kim HY, Lee JY, Kim DH. Experimental Research of Baecksunpijibujabokhap-banng (BJBB) on Atopic Dermatitis Treatment. Res Inst Korean Med. 2014 ; 22(2) : 67-79.