Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Inhibitory Effect on RANKL-Induced Osteoclast Differentiation by Water Extract of Zizyphus Jujuba Mill

대추 물 추출물이 RANKL에 의해 유도되는 파골세포 분화에 미치는 영향

  • Yoon, Kang Hugh (Department of Anatomy, Institute for Skeletal Disease, Wonkwang University) ;
  • Baek, Jong Min (Department of Anatomy, Institute for Skeletal Disease, Wonkwang University) ;
  • Kim, Ju Young (Imaging Science-based Lung and Bone Diseases Research Center, Wonkwang University) ;
  • Kwak, Seong Cheoul (Department of Anatomy, Institute for Skeletal Disease, Wonkwang University) ;
  • Cheon, Yoon Hee (Department of Anatomy, Institute for Skeletal Disease, Wonkwang University) ;
  • Jeon, Byung Hoon (Department of Pathology, College of Korean Medicine & Research Center of Traditional Korean Medicine, Wonkwang University) ;
  • Lee, Chang Hoon (Imaging Science-based Lung and Bone Diseases Research Center, Wonkwang University) ;
  • Choi, Min Kyu (Department of Anatomy, Institute for Skeletal Disease, Wonkwang University) ;
  • Oh, Jaemin (Department of Anatomy, Institute for Skeletal Disease, Wonkwang University) ;
  • Lee, Myeung Su (Imaging Science-based Lung and Bone Diseases Research Center, Wonkwang University) ;
  • Kim, Jeong Joong (Department of Anatomy, Institute for Skeletal Disease, Wonkwang University)
  • 윤강휴 (원광대학교 의과대학 해부학교실 & 골격계질환연구소) ;
  • 백종민 (원광대학교 의과대학 해부학교실 & 골격계질환연구소) ;
  • 김주영 (원광대학교 의과대학 영상의학기반 폐 및 골 질환 연구센터) ;
  • 곽성철 (원광대학교 의과대학 해부학교실 & 골격계질환연구소) ;
  • 천윤희 (원광대학교 의과대학 해부학교실 & 골격계질환연구소) ;
  • 전병훈 (원광대학교 한의과대학 병리학교실 & 한국전통의학연구소) ;
  • 이창훈 (원광대학교 의과대학 영상의학기반 폐 및 골 질환 연구센터) ;
  • 최민규 (원광대학교 의과대학 해부학교실 & 골격계질환연구소) ;
  • 오재민 (원광대학교 의과대학 해부학교실 & 골격계질환연구소) ;
  • 이명수 (원광대학교 의과대학 영상의학기반 폐 및 골 질환 연구센터) ;
  • 김정중 (원광대학교 의과대학 해부학교실 & 골격계질환연구소)
  • Received : 2013.12.06
  • Accepted : 2014.01.20
  • Published : 2014.02.25
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Abstract

Bone homeostasis is maintained by balance between bone resorbing-osteoclasts and bone forming-osteoblasts. Excessive osteoclastic bone resorption plays a critical role in bone destruction in pathological bone diseases such as osteoporosis, rheumatoid arthritis, and periodontal disease. Many compounds derived from natural products have pharmacological applications and have therapeutic value for treating or preventing several bone diseases characterized by excessive bone resorption. To discover new compounds that can act as anti-resorptive agents, we screened for natural compounds that regulate osteclast differentiation, and found that water extract of Ziziphus Jujuba Mill (WEZJ) has inhibitory effects on osteoclast differentiation. In this study, WEZJ clearly inhibits the osteoclast differentiation in the presence of receptor activator of nuclear factor kB (RANKL), macrophage colony-stimulating factor (M-CSF) without cytoxicity by blocking activation of nuclear factor of activated T cells (NFAT)c1, and c-Fos. In signaling pathway, the phosphorylation of Akt, p38, c-Jun N-terminal kinases (JNK), extracellular signal-regulated kinases (ERK) and the expression of osteoclast-associated receptor (OSCAR), tartrate-resistant acid phosphates (TRAP), Integrin av, Integrin b3, Cathepsin K are suppressed, too. These result suggest that WEZJ may have therapeutic value for treating or preventing several bone diseases characterized by excessive bone destruction.

Keywords

References

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