Journal of The Korean Dental Society of Anesthesiology (대한치과마취과학회지)

The Korean Dental Society of Anesthsiology (대한치과마취과학회)
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Effect of Propofol Preconditioning on Hypoxic-Cultured Human Osteoblast

  • Yoon, Ji Uk (Department of Anesthesia and Pain Medicine, School of Medicine, Pusan National University) ;
  • Shin, Sang Wook (Department of Anesthesia and Pain Medicine, School of Medicine, Pusan National University) ;
  • Park, Bong Soo (Department of Oral Anatomy, School of Dentistry, Pusan National University) ;
  • Kim, Yong Ho (Department of Oral Anatomy, School of Dentistry, Pusan National University) ;
  • Woo, Mi Na (Department of Dental Anesthesia and Pain Medicine, Pusan National University Dental Hospital) ;
  • Yoon, Ji Young (Department of Dental Anesthesia and Pain Medicine, School of Dentistry, Pusan National University) ;
  • Kim, Cheul Hong (Department of Dental Anesthesia and Pain Medicine, School of Dentistry, Pusan National University)
  • Received : 2014.06.24
  • Accepted : 2014.07.04
  • Published : 2014.06.30
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Abstract

Background: Angiogenesis has been recognized an essential precondition for osteogenesis. Because reduction and disruption of the blood supply to tissue cause tissue hypoxia, pathological bone loss affected by hypoxia often can occur in various clinical conditions. The effects of propofol on the process of osteogenesis have received little direct attention. Therefore, we investigated the effect of propofol on the growth and function of osteoblasts under hypoxic condition. Methods: After propofol (3, 30, $300{\mu}M$) preconditioning for 2 hours, hFOB 1.19 human osteoblast cells were cultured under 1 % oxygen tension for 48 hours. Using real time PCR and western blot analysis, we analyzed the expression of, BMP-2, TGF-${\beta}1$, type I collagen, osteocalcin, HIF-1s and Akt. Cell viability was also determined by MTT assay. Results: Propofol preconditioning on hypoxic-cultured osteoblast promoted the expressions of BMP-2, TGF-${\beta}1$, type I collagen and osteocalcin and induced hypoxia-mediated HIF-1 activation and the expression of Akt protein. Propofol with $300{\mu}M$ significant decreased cell viability compared to control. Conclusions: Clinically relevant concentrations of propofol are not cytotoxic to hypoxic osteoblasts in vitro. Propofol preconditioning on hypoxic-cultured osteoblast stimulates proliferation and differentiation of osteoblast through induced expression of BMP-2, TGF-${\beta}1$, type I collagen and osteocalcin. Propofol might promote angiogenesis and bone regeneration under hypoxic condition.

Keywords

References

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